{"id":785,"date":"2022-12-13T03:16:18","date_gmt":"2022-12-13T03:16:18","guid":{"rendered":"https:\/\/www.celetrix.com\/?page_id=785"},"modified":"2023-01-17T10:04:33","modified_gmt":"2023-01-17T10:04:33","slug":"human-primary-dendritic-cells","status":"publish","type":"page","link":"https:\/\/www.celetrix.com\/zh\/recommend-parameters\/human-primary-dendritic-cells\/","title":{"rendered":"Human primary Dendritic cells"},"content":{"rendered":"<div data-elementor-type=\"wp-page\" data-elementor-id=\"785\" class=\"elementor elementor-785\" data-elementor-post-type=\"page\">\n\t\t\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-9802eac elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"9802eac\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-50 elementor-top-column elementor-element elementor-element-c1fb32d\" data-id=\"c1fb32d\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-c22ea15 elementor-widget elementor-widget-bdt-breadcrumbs\" data-id=\"c22ea15\" data-element_type=\"widget\" data-widget_type=\"bdt-breadcrumbs.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<div class='bdt-breadcrumbs-wrapper'><ul class=\"bdt-breadcrumb breadcrumb-align-left\"><li><a href=\"https:\/\/www.celetrix.com\/zh\/\"><span class=\"bdt-breadcrumbs-home-icon\"> <i aria-hidden=\"true\" class=\"fas fa-home\"><\/i> <\/span>Electroporation by Celetrix<\/a><\/li>\n<li><span> \u9875\u9762<\/span><\/li>\n<\/ul><\/div>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-754de23 elementor-widget elementor-widget-heading\" data-id=\"754de23\" data-element_type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h2 class=\"elementor-heading-title elementor-size-default\">Human primary Dendritic cells <\/h2>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-cdfde5d elementor-widget elementor-widget-image\" data-id=\"cdfde5d\" data-element_type=\"widget\" data-widget_type=\"image.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<img fetchpriority=\"high\" decoding=\"async\" width=\"471\" height=\"181\" src=\"https:\/\/www.celetrix.com\/wp-content\/uploads\/2022\/12\/Dendritic-cells-human-primary.png\" class=\"attachment-full size-full wp-image-792\"  srcset=\"https:\/\/www.celetrix.com\/wp-content\/uploads\/2022\/12\/Dendritic-cells-human-primary.png 471w, https:\/\/www.celetrix.com\/wp-content\/uploads\/2022\/12\/Dendritic-cells-human-primary-300x115.png 300w, https:\/\/www.celetrix.com\/wp-content\/uploads\/2022\/12\/Dendritic-cells-human-primary-18x7.png 18w\" sizes=\"(max-width: 471px) 100vw, 471px\" title=\"Human primary Dendritic cells\u63d2\u56fe\" alt=\"Human primary Dendritic cells\u63d2\u56fe\" \/>\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-87a62ed elementor-widget elementor-widget-text-editor\" data-id=\"87a62ed\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<h6 style=\"text-align: center;\"><strong>Dendritic cells from PBMC, cultured for 6 days with GM-CSF and IL-4 before electroporation.<\/strong><\/h6>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-341e7cc elementor-widget elementor-widget-text-editor\" data-id=\"341e7cc\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p>\u00a0 \u00a0 \u00a0 The DC cells are easy to transfect with the Celetrix electroporation system. Follow the suggested parameters and a high transfection efficiency would be achieved.<\/p><p>\u00a0 \u00a0 \u00a0 \u00a0A special notice: DC cells have morphology variability among different donors. Cultured in GM-CSF and IL-4 for several days, sometimes they become very adherent to the plate and sometimes they are more like suspension cells. If they adhere to the plate tightly, trypsinize them down with care and try not to allow them sticking to each other and forming big clumps.\u00a0<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<section class=\"elementor-section elementor-inner-section elementor-element elementor-element-5094428 elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"5094428\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-50 elementor-inner-column elementor-element elementor-element-3321b2c\" data-id=\"3321b2c\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-5af3bcb elementor-widget elementor-widget-text-editor\" data-id=\"5af3bcb\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<h5 style=\"text-align: center;\"><strong>Human primary <b>Dendritic cells&nbsp;<\/b><\/strong><\/h5>\n<h5 style=\"text-align: center;\"><strong> in 20ul tubes<\/strong><\/h5>\n<p>&nbsp; Cell Number\uff1a1-2*10^6 cells<\/p>\n<p>&nbsp; Plasmid\uff1a2ug<\/p>\n<p>&nbsp; E buffer\uff1aCeletrix buffer<\/p>\n<p>&nbsp; Suggested parameter\uff1aCell Line Mode\uff0c480V-520V<\/p>\n<p>&nbsp; Culture medium: 1640+10%FBS&nbsp; (antibiotics free)<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t<div class=\"elementor-column elementor-col-50 elementor-inner-column elementor-element elementor-element-b53eb3a\" data-id=\"b53eb3a\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-cf62357 elementor-widget elementor-widget-text-editor\" data-id=\"cf62357\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<h5 style=\"text-align: center;\"><strong>Human primary <b>Dendritic cells\u00a0<\/b><\/strong><\/h5><h5 style=\"text-align: center;\"><strong> in 100ul tubes<\/strong><\/h5><p>\u00a0 Cell Number\uff1a5-10*10^6 cells<\/p><p>\u00a0 Plasmid\uff1a10ug<\/p><p>\u00a0 E buffer\uff1aCeletrix buffer<\/p><p>\u00a0 Suggested parameter\uff1aCell Line Mode\uff0c480V-520V<\/p><p>\u00a0 Culture medium: 1640+10%FBS\u00a0 (antibiotics free)<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t<div class=\"elementor-column elementor-col-50 elementor-top-column elementor-element elementor-element-b74975d\" data-id=\"b74975d\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-ff7e231 elementor-nav-menu__align-start elementor-nav-menu--dropdown-none elementor-widget elementor-widget-nav-menu\" data-id=\"ff7e231\" data-element_type=\"widget\" data-settings=\"{&quot;layout&quot;:&quot;vertical&quot;,&quot;submenu_icon&quot;:{&quot;value&quot;:&quot;&lt;i class=\\&quot;fas fa-caret-down\\&quot; aria-hidden=\\&quot;true\\&quot;&gt;&lt;\\\/i&gt;&quot;,&quot;library&quot;:&quot;fa-solid&quot;}}\" data-widget_type=\"nav-menu.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t<nav aria-label=\"Menu\" class=\"elementor-nav-menu--main elementor-nav-menu__container elementor-nav-menu--layout-vertical e--pointer-background e--animation-fade\">\n\t\t\t\t<ul id=\"menu-1-ff7e231\" class=\"elementor-nav-menu sm-vertical\"><li class=\"menu-item menu-item-type-post_type menu-item-object-page menu-item-105\"><a href=\"https:\/\/www.celetrix.com\/zh\/manuals-videos\/\" class=\"elementor-item\">Manuals &#038; Videos<\/a><\/li>\n<li class=\"menu-item menu-item-type-post_type menu-item-object-page menu-item-107\"><a href=\"https:\/\/www.celetrix.com\/zh\/recommend-parameters\/\" class=\"elementor-item\">Recommend Parameters<\/a><\/li>\n<li class=\"menu-item menu-item-type-post_type menu-item-object-page menu-item-106\"><a href=\"https:\/\/www.celetrix.com\/zh\/optimization-strategy\/\" class=\"elementor-item\">Optimization Strategy<\/a><\/li>\n<li class=\"menu-item menu-item-type-post_type menu-item-object-page menu-item-104\"><a href=\"https:\/\/www.celetrix.com\/zh\/experimental-overview\/\" class=\"elementor-item\">Experimental Overview<\/a><\/li>\n<li class=\"menu-item menu-item-type-post_type menu-item-object-page menu-item-108\"><a href=\"https:\/\/www.celetrix.com\/zh\/special-cells\/\" class=\"elementor-item\">Special Cells<\/a><\/li>\n<\/ul>\t\t\t<\/nav>\n\t\t\t\t\t\t<nav class=\"elementor-nav-menu--dropdown elementor-nav-menu__container\" aria-hidden=\"true\">\n\t\t\t\t<ul id=\"menu-2-ff7e231\" class=\"elementor-nav-menu sm-vertical\"><li class=\"menu-item menu-item-type-post_type menu-item-object-page menu-item-105\"><a href=\"https:\/\/www.celetrix.com\/zh\/manuals-videos\/\" class=\"elementor-item\" tabindex=\"-1\">Manuals &#038; Videos<\/a><\/li>\n<li class=\"menu-item menu-item-type-post_type menu-item-object-page menu-item-107\"><a href=\"https:\/\/www.celetrix.com\/zh\/recommend-parameters\/\" class=\"elementor-item\" tabindex=\"-1\">Recommend Parameters<\/a><\/li>\n<li class=\"menu-item menu-item-type-post_type menu-item-object-page menu-item-106\"><a href=\"https:\/\/www.celetrix.com\/zh\/optimization-strategy\/\" class=\"elementor-item\" tabindex=\"-1\">Optimization Strategy<\/a><\/li>\n<li class=\"menu-item menu-item-type-post_type menu-item-object-page menu-item-104\"><a href=\"https:\/\/www.celetrix.com\/zh\/experimental-overview\/\" class=\"elementor-item\" tabindex=\"-1\">Experimental Overview<\/a><\/li>\n<li class=\"menu-item menu-item-type-post_type menu-item-object-page menu-item-108\"><a href=\"https:\/\/www.celetrix.com\/zh\/special-cells\/\" class=\"elementor-item\" tabindex=\"-1\">Special Cells<\/a><\/li>\n<\/ul>\t\t\t<\/nav>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<\/div>","protected":false},"excerpt":{"rendered":"<p>Electroporation by Celetrix Page Human primary Dendritic cells Dendritic cells from PBMC, cultured for 6 days with GM-CSF and IL-4 before electroporation. \u00a0 \u00a0 \u00a0 The DC cells are easy to transfect with the Celetrix electroporation system. Follow the suggested parameters and a high transfection efficiency would be achieved. \u00a0 \u00a0 \u00a0 \u00a0A special notice: [&hellip;]<\/p>","protected":false},"author":1,"featured_media":0,"parent":51,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"elementor_header_footer","meta":{"om_disable_all_campaigns":false,"_uf_show_specific_survey":0,"_uf_disable_surveys":false,"footnotes":""},"class_list":["post-785","page","type-page","status-publish","hentry"],"aioseo_notices":[],"_links":{"self":[{"href":"https:\/\/www.celetrix.com\/zh\/wp-json\/wp\/v2\/pages\/785","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.celetrix.com\/zh\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/www.celetrix.com\/zh\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/www.celetrix.com\/zh\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.celetrix.com\/zh\/wp-json\/wp\/v2\/comments?post=785"}],"version-history":[{"count":22,"href":"https:\/\/www.celetrix.com\/zh\/wp-json\/wp\/v2\/pages\/785\/revisions"}],"predecessor-version":[{"id":1399,"href":"https:\/\/www.celetrix.com\/zh\/wp-json\/wp\/v2\/pages\/785\/revisions\/1399"}],"up":[{"embeddable":true,"href":"https:\/\/www.celetrix.com\/zh\/wp-json\/wp\/v2\/pages\/51"}],"wp:attachment":[{"href":"https:\/\/www.celetrix.com\/zh\/wp-json\/wp\/v2\/media?parent=785"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}