{"id":778,"date":"2022-12-13T02:57:01","date_gmt":"2022-12-13T02:57:01","guid":{"rendered":"https:\/\/www.celetrix.com\/?page_id=778"},"modified":"2026-10-23T03:45:04","modified_gmt":"2026-10-23T03:45:04","slug":"human-ipsc","status":"publish","type":"page","link":"https:\/\/www.celetrix.com\/zh\/recommend-parameters\/human-ipsc\/","title":{"rendered":"Human iPSC"},"content":{"rendered":"<div data-elementor-type=\"wp-page\" data-elementor-id=\"778\" class=\"elementor elementor-778\" data-elementor-post-type=\"page\">\n\t\t\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-207fc43 elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"207fc43\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-50 elementor-top-column elementor-element elementor-element-7ef270d\" data-id=\"7ef270d\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-6233eba elementor-widget elementor-widget-bdt-breadcrumbs\" data-id=\"6233eba\" data-element_type=\"widget\" data-widget_type=\"bdt-breadcrumbs.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<div class='bdt-breadcrumbs-wrapper'><ul class=\"bdt-breadcrumb breadcrumb-align-left\"><li><a href=\"https:\/\/www.celetrix.com\/zh\/\"><span class=\"bdt-breadcrumbs-home-icon\"> <i aria-hidden=\"true\" class=\"fas fa-home\"><\/i> <\/span>Electroporation by Celetrix<\/a><\/li>\n<li><span> \u9875\u9762<\/span><\/li>\n<\/ul><\/div>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-5899c39 elementor-widget elementor-widget-heading\" data-id=\"5899c39\" data-element_type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h4 class=\"elementor-heading-title elementor-size-default\">Human iPSC Electroporation &amp; Gene Editing Solutions<\/h4>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-42d63cc elementor-widget elementor-widget-heading\" data-id=\"42d63cc\" data-element_type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h5 class=\"elementor-heading-title elementor-size-default\">Experimental Overview &amp; Research Background<\/h5>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-cbe96fc elementor-widget elementor-widget-text-editor\" data-id=\"cbe96fc\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p><b>Overcoming Delivery Challenges in Pluripotent Stem Cells<\/b><\/p>\n<p>&nbsp;&nbsp;&nbsp; Human induced pluripotent stem cells (iPSCs) are notoriously sensitive to high-voltage stress and traditional chemical transfection methods. Celetrix&#8217;s specialized electroporation technology preserves high cell viability while achieving exceptional transient and stable delivery rates.<\/p>\n<p><b>Published Research &amp; Scientific Applications<\/b><\/p>\n<p><strong><img decoding=\"async\" style=\"color: var( --e-global-color-text ); font-family: var( --e-global-typography-text-font-family ), Sans-serif; font-size: var( --e-global-typography-text-font-size ); font-weight: var( --e-global-typography-text-font-weight );\" src=\"http:\/\/www.celetrix.com\/wp-content\/uploads\/2021\/11\/20180326165323_118.jpg\" alt=\"\" width=\"9\" height=\"8\"> <span style=\"color: var( --e-global-color-text ); font-family: var( --e-global-typography-text-font-family ), Sans-serif; font-size: var( --e-global-typography-text-font-size ); font-weight: var( --e-global-typography-text-font-weight );\">&nbsp;<\/span>Johns Hopkins University School of Medicine (Disease Modeling)<\/strong>: Researchers successfully generated and characterized human homozygous SCA12 iPSC lines (such as line JHUi003-A) utilizing Celetrix electroporation systems.<\/p>\n<p><strong><img decoding=\"async\" style=\"color: var( --e-global-color-text ); font-family: var( --e-global-typography-text-font-family ), Sans-serif; font-size: var( --e-global-typography-text-font-size ); font-weight: var( --e-global-typography-text-font-weight );\" src=\"http:\/\/www.celetrix.com\/wp-content\/uploads\/2021\/11\/20180326165323_118.jpg\" alt=\"\" width=\"9\" height=\"8\"> <span style=\"color: var( --e-global-color-text ); font-family: var( --e-global-typography-text-font-family ), Sans-serif; font-size: var( --e-global-typography-text-font-size ); font-weight: var( --e-global-typography-text-font-weight );\">&nbsp;<\/span>Scientific Reports (Nature Publishing Group) (CRISPR &amp; HDR Enhancement)<\/strong>: Studies demonstrate that combining Celetrix electroporation with transient BCL-XL overexpression and single-guided Cas9 nickase (Cas9n) significantly boosts post-transfection cell survival and achieves high-efficiency homology-directed repair (HDR) in human pluripotent stem cells.<\/p>\n<p><strong><img decoding=\"async\" style=\"color: var( --e-global-color-text ); font-family: var( --e-global-typography-text-font-family ), Sans-serif; font-size: var( --e-global-typography-text-font-size ); font-weight: var( --e-global-typography-text-font-weight );\" src=\"http:\/\/www.celetrix.com\/wp-content\/uploads\/2021\/11\/20180326165323_118.jpg\" alt=\"\" width=\"9\" height=\"8\"> <span style=\"color: var( --e-global-color-text ); font-family: var( --e-global-typography-text-font-family ), Sans-serif; font-size: var( --e-global-typography-text-font-size ); font-weight: var( --e-global-typography-text-font-weight );\">&nbsp;<\/span>Pluripotency Preservation<\/strong>: Gene-edited iPSCs maintain normal chromosomal karyotypes, strongly express classic markers (e.g., Oct4A, Sox2, Nanog, SSEA4, TRA-1-60), and retain full multi-lineage differentiation capacity.<\/p>\n<p>For CRISPR knockout in iPSCs, see our <a href=\"\/zh\/stem-cell-therapy\/\">iPSC gene editing protocol (Cas9 RNP)<\/a>.<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-4dad6fc elementor-widget elementor-widget-image\" data-id=\"4dad6fc\" data-element_type=\"widget\" data-widget_type=\"image.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<img fetchpriority=\"high\" decoding=\"async\" width=\"414\" height=\"200\" src=\"https:\/\/www.celetrix.com\/wp-content\/uploads\/celetrix-acs1030-undifferentiated-ipsc-colony-72h.jpg\" class=\"attachment-full size-full wp-image-1923\" alt=\"Microscopy images demonstrating sustained GFP expression in intact human iPSC colonies with typical undifferentiated morphology 72 hours after Celetrix electroporation.\" srcset=\"https:\/\/www.celetrix.com\/wp-content\/uploads\/celetrix-acs1030-undifferentiated-ipsc-colony-72h.jpg 414w, https:\/\/www.celetrix.com\/wp-content\/uploads\/celetrix-acs1030-undifferentiated-ipsc-colony-72h-300x145.jpg 300w, https:\/\/www.celetrix.com\/wp-content\/uploads\/celetrix-acs1030-undifferentiated-ipsc-colony-72h-18x9.jpg 18w\" sizes=\"(max-width: 414px) 100vw, 414px\" \/>\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<section class=\"elementor-section elementor-inner-section elementor-element elementor-element-6bbf7b8 elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"6bbf7b8\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-33 elementor-inner-column elementor-element elementor-element-f4d6bf3\" data-id=\"f4d6bf3\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap\">\n\t\t\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t<div class=\"elementor-column elementor-col-33 elementor-inner-column elementor-element elementor-element-16ff997\" data-id=\"16ff997\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-9bb1470 elementor-widget__width-initial elementor-widget elementor-widget-text-editor\" data-id=\"9bb1470\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p style=\"text-align: center;\"><strong>High-efficiency transfection and continuous culture of human iPSCs using Celetrix technology.<\/strong><\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t<div class=\"elementor-column elementor-col-33 elementor-inner-column elementor-element elementor-element-b86ad07\" data-id=\"b86ad07\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap\">\n\t\t\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-inner-section elementor-element elementor-element-3c1d19c elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"3c1d19c\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-50 elementor-inner-column elementor-element elementor-element-26c9d16\" data-id=\"26c9d16\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-63f545c elementor-widget elementor-widget-text-editor\" data-id=\"63f545c\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<h5 style=\"text-align: center;\"><strong>Human iPSC in 20ul tubes<\/strong><\/h5><p>\u00a0 Cell Number\uff1a1-2*10^6 cells<\/p><p>\u00a0 Plasmid\uff1a2ug<\/p><p>\u00a0 E buffer\uff1aCeletrix buffer<\/p><p>\u00a0 Suggested parameter\uff1aCell Line Mode\uff0c500V-540V<\/p><p>\u00a0 Culture medium: Antibiotic-free Human iPSC culture medium<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t<div class=\"elementor-column elementor-col-50 elementor-inner-column elementor-element elementor-element-b37b6d5\" data-id=\"b37b6d5\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-113bedd elementor-widget elementor-widget-text-editor\" data-id=\"113bedd\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<h5 style=\"text-align: center;\"><strong>Human iPSC in 100ul tubes<\/strong><\/h5><p>\u00a0 Cell Number\uff1a5-10*10^6 cells<\/p><p>\u00a0 Plasmid\uff1a10ug<\/p><p>\u00a0 E buffer\uff1aCeletrix buffer<\/p><p>\u00a0 Suggested parameter\uff1aCell Line Mode\uff0c500V-540V<\/p><p>\u00a0 Culture medium: Antibiotic-free Human iPSC culture medium<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t<div class=\"elementor-column elementor-col-50 elementor-top-column elementor-element elementor-element-76ce125\" data-id=\"76ce125\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-fd40c63 elementor-nav-menu__align-start elementor-nav-menu--dropdown-none elementor-widget elementor-widget-nav-menu\" data-id=\"fd40c63\" 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menu-item-105\"><a href=\"https:\/\/www.celetrix.com\/zh\/manuals-videos\/\" class=\"elementor-item\">Manuals &#038; Videos<\/a><\/li>\n<li class=\"menu-item menu-item-type-post_type menu-item-object-page menu-item-107\"><a href=\"https:\/\/www.celetrix.com\/zh\/recommend-parameters\/\" class=\"elementor-item\">Recommended Parameters<\/a><\/li>\n<li class=\"menu-item menu-item-type-post_type menu-item-object-page menu-item-106\"><a href=\"https:\/\/www.celetrix.com\/zh\/optimization-strategy\/\" class=\"elementor-item\">Optimization Strategy<\/a><\/li>\n<li class=\"menu-item menu-item-type-post_type menu-item-object-page menu-item-104\"><a href=\"https:\/\/www.celetrix.com\/zh\/experimental-overview\/\" class=\"elementor-item\">Experimental Overview<\/a><\/li>\n<li class=\"menu-item menu-item-type-post_type menu-item-object-page menu-item-108\"><a href=\"https:\/\/www.celetrix.com\/zh\/special-cells\/\" class=\"elementor-item\">Special Cells<\/a><\/li>\n<\/ul>\t\t\t<\/nav>\n\t\t\t\t\t\t<nav class=\"elementor-nav-menu--dropdown elementor-nav-menu__container\" aria-hidden=\"true\">\n\t\t\t\t<ul id=\"menu-2-fd40c63\" class=\"elementor-nav-menu sm-vertical\"><li class=\"menu-item menu-item-type-post_type menu-item-object-page menu-item-105\"><a href=\"https:\/\/www.celetrix.com\/zh\/manuals-videos\/\" class=\"elementor-item\" tabindex=\"-1\">Manuals &#038; Videos<\/a><\/li>\n<li class=\"menu-item menu-item-type-post_type menu-item-object-page menu-item-107\"><a href=\"https:\/\/www.celetrix.com\/zh\/recommend-parameters\/\" class=\"elementor-item\" tabindex=\"-1\">Recommended Parameters<\/a><\/li>\n<li class=\"menu-item menu-item-type-post_type menu-item-object-page menu-item-106\"><a href=\"https:\/\/www.celetrix.com\/zh\/optimization-strategy\/\" class=\"elementor-item\" tabindex=\"-1\">Optimization Strategy<\/a><\/li>\n<li class=\"menu-item menu-item-type-post_type menu-item-object-page menu-item-104\"><a href=\"https:\/\/www.celetrix.com\/zh\/experimental-overview\/\" class=\"elementor-item\" tabindex=\"-1\">Experimental Overview<\/a><\/li>\n<li class=\"menu-item menu-item-type-post_type menu-item-object-page menu-item-108\"><a href=\"https:\/\/www.celetrix.com\/zh\/special-cells\/\" class=\"elementor-item\" tabindex=\"-1\">Special Cells<\/a><\/li>\n<\/ul>\t\t\t<\/nav>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<\/div>","protected":false},"excerpt":{"rendered":"<p>Electroporation by Celetrix Page Human iPSC Electroporation &amp; Gene Editing Solutions Experimental Overview &amp; Research Background Overcoming Delivery Challenges in Pluripotent Stem Cells &nbsp;&nbsp;&nbsp; Human induced pluripotent stem cells (iPSCs) are notoriously sensitive to high-voltage stress and traditional chemical transfection methods. Celetrix&#8217;s specialized electroporation technology preserves high cell viability while achieving exceptional transient and stable [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"parent":51,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"elementor_header_footer","meta":{"om_disable_all_campaigns":false,"_uf_show_specific_survey":0,"_uf_disable_surveys":false,"footnotes":""},"class_list":["post-778","page","type-page","status-publish","hentry"],"aioseo_notices":[],"_links":{"self":[{"href":"https:\/\/www.celetrix.com\/zh\/wp-json\/wp\/v2\/pages\/778","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.celetrix.com\/zh\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/www.celetrix.com\/zh\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/www.celetrix.com\/zh\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.celetrix.com\/zh\/wp-json\/wp\/v2\/comments?post=778"}],"version-history":[{"count":34,"href":"https:\/\/www.celetrix.com\/zh\/wp-json\/wp\/v2\/pages\/778\/revisions"}],"predecessor-version":[{"id":2497,"href":"https:\/\/www.celetrix.com\/zh\/wp-json\/wp\/v2\/pages\/778\/revisions\/2497"}],"up":[{"embeddable":true,"href":"https:\/\/www.celetrix.com\/zh\/wp-json\/wp\/v2\/pages\/51"}],"wp:attachment":[{"href":"https:\/\/www.celetrix.com\/zh\/wp-json\/wp\/v2\/media?parent=778"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}