{"id":764,"date":"2022-12-13T02:44:54","date_gmt":"2022-12-13T02:44:54","guid":{"rendered":"https:\/\/www.celetrix.com\/?page_id=764"},"modified":"2026-10-16T08:20:37","modified_gmt":"2026-10-16T08:20:37","slug":"cultured-nk-cells","status":"publish","type":"page","link":"https:\/\/www.celetrix.com\/zh\/recommend-parameters\/cultured-nk-cells\/","title":{"rendered":"Human Cultured NK cells"},"content":{"rendered":"<div data-elementor-type=\"wp-page\" data-elementor-id=\"764\" class=\"elementor elementor-764\" data-elementor-post-type=\"page\">\n\t\t\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-75a3d14 elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"75a3d14\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-50 elementor-top-column elementor-element elementor-element-cb3fc29\" data-id=\"cb3fc29\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-f954179 elementor-widget elementor-widget-bdt-breadcrumbs\" data-id=\"f954179\" data-element_type=\"widget\" data-widget_type=\"bdt-breadcrumbs.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<div class='bdt-breadcrumbs-wrapper'><ul class=\"bdt-breadcrumb breadcrumb-align-left\"><li><a href=\"https:\/\/www.celetrix.com\/zh\/\"><span class=\"bdt-breadcrumbs-home-icon\"> <i aria-hidden=\"true\" class=\"fas fa-home\"><\/i> <\/span>Electroporation by Celetrix<\/a><\/li>\n<li><span> \u9875\u9762<\/span><\/li>\n<\/ul><\/div>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-efa1ebb elementor-widget elementor-widget-heading\" data-id=\"efa1ebb\" data-element_type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h4 class=\"elementor-heading-title elementor-size-default\">Human Cultured NK Cells Electroporation Protocol &amp; Parameters    <\/h4>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-2afc460 elementor-widget elementor-widget-heading\" data-id=\"2afc460\" data-element_type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h5 class=\"elementor-heading-title elementor-size-default\">Technical Overview &amp; Cell State Insights        <\/h5>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-c5b45f5 elementor-widget elementor-widget-text-editor\" data-id=\"c5b45f5\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p>\u00a0 \u00a0 Electroporation parameters for primary purified natural killer (NK) cells and peripheral blood mononuclear cells (PBMCs) share similar characteristics. In their unstimulated, resting state, primary NK cells have a small diameter of approximately <b>7\u20138 <\/b>\u03bcm, requiring significantly higher voltages than standard mammalian cell lines. Consequently, their transfection protocols can directly follow standard <span style=\"text-decoration: underline;\"><span style=\"color: #20709e; text-decoration: underline;\"><a style=\"color: #20709e; text-decoration: underline;\" href=\"https:\/\/www.celetrix.com\/zh\/recommend-parameters\/\">PBMC electroporation guidelines<\/a>.<\/span><\/span><\/p><p>\u00a0 \u00a0 However, <b>stimulated and cultured NK cells<\/b> increase significantly in size during proliferation. Because larger cells require lower electric field strengths, cultured NK cells need much lower voltages than resting primary NK cells\u2014comparable to standard suspension cell lines\u2014and can be effectively transfected using the <b>Cell Line Mode<\/b> on Celetrix systems.<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-4f8f94c elementor-widget elementor-widget-heading\" data-id=\"4f8f94c\" data-element_type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h6 class=\"elementor-heading-title elementor-size-default\">Optimization Highlights              <\/h6>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-89d4dee elementor-widget elementor-widget-text-editor\" data-id=\"89d4dee\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p><img decoding=\"async\" style=\"color: var( --e-global-color-text ); font-family: var( --e-global-typography-text-font-family ), Sans-serif; font-size: var( --e-global-typography-text-font-size ); font-weight: var( --e-global-typography-text-font-weight );\" src=\"http:\/\/www.celetrix.com\/wp-content\/uploads\/2021\/11\/20180326165323_118.jpg\" alt=\"\" width=\"9\" height=\"8\"> <span style=\"color: var( --e-global-color-text ); font-family: var( --e-global-typography-text-font-family ), Sans-serif; font-size: var( --e-global-typography-text-font-size ); font-weight: var( --e-global-typography-text-font-weight );\">&nbsp;<\/span><b>Stimulation Methods:<\/b> We evaluated various culture stimulation protocols and found that both&nbsp;<b>feeder cell-co-culture<\/b>&nbsp;and liquid-only media works. Feeder cell protocol may yield better results compared to liquid-only media.<\/p>\n<p><img decoding=\"async\" style=\"color: var( --e-global-color-text ); font-family: var( --e-global-typography-text-font-family ), Sans-serif; font-size: var( --e-global-typography-text-font-size ); font-weight: var( --e-global-typography-text-font-weight );\" src=\"http:\/\/www.celetrix.com\/wp-content\/uploads\/2021\/11\/20180326165323_118.jpg\" alt=\"\" width=\"9\" height=\"8\"><span style=\"color: var( --e-global-color-text ); font-family: var( --e-global-typography-text-font-family ), Sans-serif; font-size: var( --e-global-typography-text-font-size ); font-weight: var( --e-global-typography-text-font-weight );\"> &nbsp;<\/span><b>Culture Duration:<\/b> To achieve high transfection efficiency while maintaining optimal cell viability, <b>NK cells are typically stimulated for 5-7 days prior to electroporation<\/b>.<\/p>\n<p><img decoding=\"async\" style=\"color: var( --e-global-color-text ); font-family: var( --e-global-typography-text-font-family ), Sans-serif; font-size: var( --e-global-typography-text-font-size ); font-weight: var( --e-global-typography-text-font-weight );\" src=\"http:\/\/www.celetrix.com\/wp-content\/uploads\/2021\/11\/20180326165323_118.jpg\" alt=\"\" width=\"9\" height=\"8\"><span style=\"color: var( --e-global-color-text ); font-family: var( --e-global-typography-text-font-family ), Sans-serif; font-size: var( --e-global-typography-text-font-size ); font-weight: var( --e-global-typography-text-font-weight );\"> &nbsp;<\/span><b>Key Variable:<\/b> Because stimulation conditions directly impact cell size and viability, culture duration and electroporation voltage should be carefully optimized for your specific experimental workflow.<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-465da5c elementor-widget elementor-widget-heading\" data-id=\"465da5c\" data-element_type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h6 class=\"elementor-heading-title elementor-size-default\">Performance Benchmark                              <\/h6>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-85e3099 elementor-widget elementor-widget-text-editor\" data-id=\"85e3099\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p>NK cells stimulated for 6 days achieved an <b>80% GFP-positive rate<\/b> at 48 hours post-electroporation with a GFP transposon, sustaining <b>50% positivity<\/b> through 8 days of continued culture.<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-b14e3fc elementor-widget elementor-widget-heading\" data-id=\"b14e3fc\" data-element_type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h6 class=\"elementor-heading-title elementor-size-default\">Experimental Performance &amp; Flow Cytometry Data                   <\/h6>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-06e0323 elementor-widget elementor-widget-text-editor\" data-id=\"06e0323\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p style=\"text-align: center;\">\u00a0 \u00a0 \u00a0<strong> 48h Post-Transfection (Day 6 Stimulation)<\/strong><\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-d550083 elementor-widget elementor-widget-image\" data-id=\"d550083\" data-element_type=\"widget\" data-widget_type=\"image.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<img fetchpriority=\"high\" decoding=\"async\" width=\"494\" height=\"244\" src=\"https:\/\/www.celetrix.com\/wp-content\/uploads\/Celetrix-Electroporation-System-Human-Cultured-NK-Cells-48-Hour-Transfection-Efficiency.png\" class=\"attachment-full size-full wp-image-2283\" alt=\"Flow cytometry analysis using the Celetrix electroporation system, demonstrating a high transfection efficiency with an 80.52 percent GFP-positive rate at 48 hours post-electroporation in cultured NK cells.\" srcset=\"https:\/\/www.celetrix.com\/wp-content\/uploads\/Celetrix-Electroporation-System-Human-Cultured-NK-Cells-48-Hour-Transfection-Efficiency.png 494w, https:\/\/www.celetrix.com\/wp-content\/uploads\/Celetrix-Electroporation-System-Human-Cultured-NK-Cells-48-Hour-Transfection-Efficiency-300x148.png 300w, https:\/\/www.celetrix.com\/wp-content\/uploads\/Celetrix-Electroporation-System-Human-Cultured-NK-Cells-48-Hour-Transfection-Efficiency-18x9.png 18w\" sizes=\"(max-width: 494px) 100vw, 494px\" \/>\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-9db600e elementor-widget elementor-widget-text-editor\" data-id=\"9db600e\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p>\u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0FSC vs. SSC Plot\u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 GFP Efficiency (80.52%)<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-a7fc287 elementor-widget elementor-widget-text-editor\" data-id=\"a7fc287\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p style=\"text-align: center;\"><strong>\u00a0 \u00a0Day 8 Post-Culture (Long-Term Expression)<\/strong><\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-ca9c1d1 elementor-widget elementor-widget-image\" data-id=\"ca9c1d1\" data-element_type=\"widget\" data-widget_type=\"image.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<img decoding=\"async\" width=\"453\" height=\"222\" src=\"https:\/\/www.celetrix.com\/wp-content\/uploads\/Celetrix-Electroporation-Human-Cultured-NK-Cells-Day-8-Post-Culture-Sustained-Expression.png\" class=\"attachment-full size-full wp-image-2287\" alt=\"Flow cytometry scatter plots showing sustained transgene expression after Celetrix electroporation, with the GFP-positive rate remaining at 50.27 percent after 8 days of continued culture in human NK cells.\" srcset=\"https:\/\/www.celetrix.com\/wp-content\/uploads\/Celetrix-Electroporation-Human-Cultured-NK-Cells-Day-8-Post-Culture-Sustained-Expression.png 453w, https:\/\/www.celetrix.com\/wp-content\/uploads\/Celetrix-Electroporation-Human-Cultured-NK-Cells-Day-8-Post-Culture-Sustained-Expression-300x147.png 300w, https:\/\/www.celetrix.com\/wp-content\/uploads\/Celetrix-Electroporation-Human-Cultured-NK-Cells-Day-8-Post-Culture-Sustained-Expression-18x9.png 18w\" sizes=\"(max-width: 453px) 100vw, 453px\" \/>\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-69e1e83 elementor-widget elementor-widget-text-editor\" data-id=\"69e1e83\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p>\u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 Extended Culture Scatter Plot\u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 Sustained Expression (50.27%)<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<section class=\"elementor-section elementor-inner-section elementor-element elementor-element-3c5cffb elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"3c5cffb\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-50 elementor-inner-column elementor-element elementor-element-34609f8\" data-id=\"34609f8\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-4a8977f elementor-widget elementor-widget-text-editor\" data-id=\"4a8977f\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<h5 style=\"text-align: center;\"><strong>Human Cultured NK cells in 20ul tubes<\/strong><\/h5><p>\u00a0 Cell Number\uff1a1-2*10^6 cells<\/p><p>\u00a0 Plasmid\uff1a2ug<\/p><p>\u00a0 E buffer\uff1aCeletrix buffer<\/p><p>\u00a0 Suggested parameter\uff1aCell Line Mode\uff0c480V-540V<\/p><p>\u00a0 Culture medium: Antibiotic-free NK cell culture medium<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t<div class=\"elementor-column elementor-col-50 elementor-inner-column elementor-element elementor-element-b8dc9f6\" data-id=\"b8dc9f6\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-15ad8a4 elementor-widget elementor-widget-text-editor\" data-id=\"15ad8a4\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<h5 style=\"text-align: center;\"><strong>Human Cultured NK cells in 100ul tubes<\/strong><\/h5><p>\u00a0 Cell Number\uff1a5-10*10^6 cells<\/p><p>\u00a0 Plasmid\uff1a10ug<\/p><p>\u00a0 E buffer\uff1aCeletrix buffer<\/p><p>\u00a0 Suggested parameter\uff1aCell Line Mode\uff0c480V-540V<\/p><p>\u00a0 Culture medium: Antibiotic-free NK cell culture medium<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t<div class=\"elementor-column elementor-col-50 elementor-top-column elementor-element elementor-element-12880d5\" data-id=\"12880d5\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-d420a09 elementor-nav-menu__align-start elementor-nav-menu--dropdown-none elementor-widget elementor-widget-nav-menu\" data-id=\"d420a09\" data-element_type=\"widget\" data-settings=\"{&quot;layout&quot;:&quot;vertical&quot;,&quot;submenu_icon&quot;:{&quot;value&quot;:&quot;&lt;svg aria-hidden=\\&quot;true\\&quot; class=\\&quot;e-font-icon-svg e-fas-caret-down\\&quot; viewBox=\\&quot;0 0 320 512\\&quot; xmlns=\\&quot;http:\\\/\\\/www.w3.org\\\/2000\\\/svg\\&quot;&gt;&lt;path d=\\&quot;M31.3 192h257.3c17.8 0 26.7 21.5 14.1 34.1L174.1 354.8c-7.8 7.8-20.5 7.8-28.3 0L17.2 226.1C4.6 213.5 13.5 192 31.3 192z\\&quot;&gt;&lt;\\\/path&gt;&lt;\\\/svg&gt;&quot;,&quot;library&quot;:&quot;fa-solid&quot;}}\" data-widget_type=\"nav-menu.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t<nav aria-label=\"Menu\" class=\"elementor-nav-menu--main elementor-nav-menu__container elementor-nav-menu--layout-vertical e--pointer-background e--animation-fade\">\n\t\t\t\t<ul id=\"menu-1-d420a09\" class=\"elementor-nav-menu sm-vertical\"><li class=\"menu-item menu-item-type-post_type menu-item-object-page menu-item-105\"><a href=\"https:\/\/www.celetrix.com\/zh\/manuals-videos\/\" class=\"elementor-item\">Manuals &#038; Videos<\/a><\/li>\n<li class=\"menu-item menu-item-type-post_type menu-item-object-page menu-item-107\"><a href=\"https:\/\/www.celetrix.com\/zh\/recommend-parameters\/\" class=\"elementor-item\">Recommended Parameters<\/a><\/li>\n<li class=\"menu-item menu-item-type-post_type menu-item-object-page menu-item-106\"><a href=\"https:\/\/www.celetrix.com\/zh\/optimization-strategy\/\" class=\"elementor-item\">Optimization Strategy<\/a><\/li>\n<li class=\"menu-item menu-item-type-post_type menu-item-object-page menu-item-104\"><a href=\"https:\/\/www.celetrix.com\/zh\/experimental-overview\/\" class=\"elementor-item\">Experimental Overview<\/a><\/li>\n<li class=\"menu-item menu-item-type-post_type menu-item-object-page menu-item-108\"><a href=\"https:\/\/www.celetrix.com\/zh\/special-cells\/\" class=\"elementor-item\">Special Cells<\/a><\/li>\n<\/ul>\t\t\t<\/nav>\n\t\t\t\t\t\t<nav class=\"elementor-nav-menu--dropdown elementor-nav-menu__container\" aria-hidden=\"true\">\n\t\t\t\t<ul id=\"menu-2-d420a09\" class=\"elementor-nav-menu sm-vertical\"><li class=\"menu-item menu-item-type-post_type menu-item-object-page menu-item-105\"><a href=\"https:\/\/www.celetrix.com\/zh\/manuals-videos\/\" class=\"elementor-item\" tabindex=\"-1\">Manuals &#038; Videos<\/a><\/li>\n<li class=\"menu-item menu-item-type-post_type menu-item-object-page menu-item-107\"><a href=\"https:\/\/www.celetrix.com\/zh\/recommend-parameters\/\" class=\"elementor-item\" tabindex=\"-1\">Recommended Parameters<\/a><\/li>\n<li class=\"menu-item menu-item-type-post_type menu-item-object-page menu-item-106\"><a href=\"https:\/\/www.celetrix.com\/zh\/optimization-strategy\/\" class=\"elementor-item\" tabindex=\"-1\">Optimization Strategy<\/a><\/li>\n<li class=\"menu-item menu-item-type-post_type menu-item-object-page menu-item-104\"><a href=\"https:\/\/www.celetrix.com\/zh\/experimental-overview\/\" class=\"elementor-item\" tabindex=\"-1\">Experimental Overview<\/a><\/li>\n<li class=\"menu-item menu-item-type-post_type menu-item-object-page menu-item-108\"><a href=\"https:\/\/www.celetrix.com\/zh\/special-cells\/\" class=\"elementor-item\" tabindex=\"-1\">Special Cells<\/a><\/li>\n<\/ul>\t\t\t<\/nav>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<\/div>","protected":false},"excerpt":{"rendered":"<p>Electroporation by Celetrix Page Human Cultured NK Cells Electroporation Protocol &amp; Parameters Technical Overview &amp; Cell State Insights \u00a0 \u00a0 Electroporation parameters for primary purified natural killer (NK) cells and peripheral blood mononuclear cells (PBMCs) share similar characteristics. In their unstimulated, resting state, primary NK cells have a small diameter of approximately 7\u20138 \u03bcm, requiring [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"parent":51,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"elementor_header_footer","meta":{"om_disable_all_campaigns":false,"_uf_show_specific_survey":0,"_uf_disable_surveys":false,"footnotes":""},"class_list":["post-764","page","type-page","status-publish","hentry"],"aioseo_notices":[],"_links":{"self":[{"href":"https:\/\/www.celetrix.com\/zh\/wp-json\/wp\/v2\/pages\/764","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.celetrix.com\/zh\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/www.celetrix.com\/zh\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/www.celetrix.com\/zh\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.celetrix.com\/zh\/wp-json\/wp\/v2\/comments?post=764"}],"version-history":[{"count":61,"href":"https:\/\/www.celetrix.com\/zh\/wp-json\/wp\/v2\/pages\/764\/revisions"}],"predecessor-version":[{"id":2347,"href":"https:\/\/www.celetrix.com\/zh\/wp-json\/wp\/v2\/pages\/764\/revisions\/2347"}],"up":[{"embeddable":true,"href":"https:\/\/www.celetrix.com\/zh\/wp-json\/wp\/v2\/pages\/51"}],"wp:attachment":[{"href":"https:\/\/www.celetrix.com\/zh\/wp-json\/wp\/v2\/media?parent=764"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}