{"id":764,"date":"2022-12-13T02:44:54","date_gmt":"2022-12-13T02:44:54","guid":{"rendered":"https:\/\/www.celetrix.com\/?page_id=764"},"modified":"2023-01-13T07:27:27","modified_gmt":"2023-01-13T07:27:27","slug":"cultured-nk-cells","status":"publish","type":"page","link":"https:\/\/www.celetrix.com\/zh\/recommend-parameters\/cultured-nk-cells\/","title":{"rendered":"Human Cultured NK cells"},"content":{"rendered":"<div data-elementor-type=\"wp-page\" data-elementor-id=\"764\" class=\"elementor elementor-764\" data-elementor-post-type=\"page\">\n\t\t\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-75a3d14 elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"75a3d14\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-50 elementor-top-column elementor-element elementor-element-cb3fc29\" data-id=\"cb3fc29\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-f954179 elementor-widget elementor-widget-bdt-breadcrumbs\" data-id=\"f954179\" data-element_type=\"widget\" data-widget_type=\"bdt-breadcrumbs.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<div class='bdt-breadcrumbs-wrapper'><ul class=\"bdt-breadcrumb breadcrumb-align-left\"><li><a href=\"https:\/\/www.celetrix.com\/zh\/\"><span class=\"bdt-breadcrumbs-home-icon\"> <i aria-hidden=\"true\" class=\"fas fa-home\"><\/i> <\/span>Electroporation by Celetrix<\/a><\/li>\n<li><span> \u9875\u9762<\/span><\/li>\n<\/ul><\/div>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-8224971 elementor-widget elementor-widget-heading\" data-id=\"8224971\" data-element_type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h2 class=\"elementor-heading-title elementor-size-default\">Human Cultured NK  cells\n<\/h2>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-c5b45f5 elementor-widget elementor-widget-text-editor\" data-id=\"c5b45f5\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p style=\"margin-top: 0pt; margin-bottom: 0pt; margin-left: 0in; direction: ltr; unicode-bidi: embed; vertical-align: baseline; word-break: normal;\"><span style=\"font-size: 10.0pt; font-family: Calibri; mso-ascii-font-family: Calibri; mso-fareast-font-family: \u5b8b\u4f53; mso-bidi-font-family: +mn-cs; mso-ascii-theme-font: minor-latin; mso-fareast-theme-font: minor-fareast; mso-bidi-theme-font: minor-bidi; color: black; mso-color-index: 1; mso-font-kerning: 12.0pt; language: zh-CN; mso-style-textfill-type: solid; mso-style-textfill-fill-themecolor: text1; mso-style-textfill-fill-color: black; mso-style-textfill-fill-alpha: 100.0%;\">\u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u5728<\/span><span style=\"font-size: 10.0pt; font-family: \u5b8b\u4f53; mso-ascii-font-family: Calibri; mso-fareast-font-family: \u5b8b\u4f53; mso-bidi-font-family: +mn-cs; mso-ascii-theme-font: minor-latin; mso-fareast-theme-font: minor-fareast; mso-bidi-theme-font: minor-bidi; color: black; mso-color-index: 1; mso-font-kerning: 12.0pt; language: zh-CN; mso-style-textfill-type: solid; mso-style-textfill-fill-themecolor: text1; mso-style-textfill-fill-color: black; mso-style-textfill-fill-alpha: 100.0%;\">\u7535\u8f6c\u6761\u4ef6\u4e0a<\/span><span style=\"font-size: 10.0pt; font-family: \u5b8b\u4f53; mso-ascii-font-family: Calibri; mso-fareast-font-family: \u5b8b\u4f53; mso-bidi-font-family: +mn-cs; mso-ascii-theme-font: minor-latin; mso-fareast-theme-font: minor-fareast; mso-bidi-theme-font: 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mso-style-textfill-type: solid; mso-style-textfill-fill-themecolor: text1; mso-style-textfill-fill-color: black; mso-style-textfill-fill-alpha: 100.0%;\">\u5f88\u591a\uff0c\u53ef\u4ee5\u76f4\u63a5\u53c2\u7167<\/span><span style=\"font-size: 10.0pt; font-family: Calibri; mso-ascii-font-family: Calibri; mso-fareast-font-family: \u5b8b\u4f53; mso-bidi-font-family: +mn-cs; mso-ascii-theme-font: minor-latin; mso-fareast-theme-font: minor-fareast; mso-bidi-theme-font: minor-bidi; color: black; mso-color-index: 1; mso-font-kerning: 12.0pt; language: en-US; mso-style-textfill-type: solid; mso-style-textfill-fill-themecolor: text1; mso-style-textfill-fill-color: black; mso-style-textfill-fill-alpha: 100.0%;\">PBMC<\/span><span style=\"font-size: 10.0pt; font-family: \u5b8b\u4f53; mso-ascii-font-family: Calibri; mso-fareast-font-family: \u5b8b\u4f53; mso-bidi-font-family: +mn-cs; mso-ascii-theme-font: minor-latin; mso-fareast-theme-font: minor-fareast; mso-bidi-theme-font: minor-bidi; color: 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style=\"font-size: 10pt; font-family: \u5b8b\u4f53; font-variant-numeric: normal; font-variant-east-asian: normal; color: black; vertical-align: baseline;\">\u7ec6\u80de\u4f4e\u5f88\u591a\uff0c\u548c<\/span><span style=\"font-size: 10.0pt; font-family: \u5b8b\u4f53; mso-ascii-font-family: Verdana; mso-fareast-font-family: \u5b8b\u4f53; mso-bidi-font-family: \u5b8b\u4f53; color: black; mso-font-kerning: 12.0pt; language: zh-CN; mso-style-textfill-type: solid; mso-style-textfill-fill-color: black; mso-style-textfill-fill-alpha: 100.0%;\">\u666e\u901a\u7ec6\u80de\u7cfb\u7535\u8f6c\u7535\u538b\u7c7b\u4f3c\uff0c\u4f7f\u7528<\/span><span style=\"font-size: 10.0pt; font-family: Verdana; mso-ascii-font-family: Verdana; mso-fareast-font-family: \u5b8b\u4f53; mso-bidi-font-family: \u5b8b\u4f53; color: black; mso-font-kerning: 12.0pt; language: en-US; mso-style-textfill-type: solid; mso-style-textfill-fill-color: black; mso-style-textfill-fill-alpha: 100.0%;\">Cell line<\/span><span style=\"font-size: 10.0pt; font-family: \u5b8b\u4f53; mso-ascii-font-family: Verdana; mso-fareast-font-family: \u5b8b\u4f53; mso-bidi-font-family: \u5b8b\u4f53; color: black; mso-font-kerning: 12.0pt; language: zh-CN; mso-style-textfill-type: solid; mso-style-textfill-fill-color: black; mso-style-textfill-fill-alpha: 100.0%;\">\u6a21\u5f0f\u7535\u8f6c\u5373\u53ef\u3002<\/span><\/p><p style=\"margin-top: 0pt; margin-bottom: 0pt; margin-left: 0in; direction: ltr; unicode-bidi: embed; vertical-align: baseline; word-break: normal;\">\u00a0<\/p><p style=\"line-height: normal; margin: 0pt 0in; text-indent: 0in; direction: ltr; unicode-bidi: embed; vertical-align: baseline; word-break: normal;\">\u00a0<\/p><p style=\"language: zh-CN; margin-top: 0pt; margin-bottom: 0pt; margin-left: 0in; text-align: left; direction: ltr; unicode-bidi: embed; vertical-align: baseline; mso-line-break-override: restrictions; word-break: normal; punctuation-wrap: simple;\"><span style=\"font-size: 10.0pt; font-family: Calibri; mso-ascii-font-family: Calibri; mso-fareast-font-family: \u5b8b\u4f53; mso-bidi-font-family: \u5b8b\u4f53; font-variant: normal; color: black; text-transform: none; mso-font-kerning: 12.0pt; language: zh-CN; font-weight: normal; font-style: normal; vertical-align: baseline; mso-text-raise: 0%; mso-style-textoutline-type: none; mso-style-textfill-type: solid; mso-style-textfill-fill-color: black; mso-style-textfill-fill-alpha: 100.0%;\">\u00a0<\/span> <span style=\"font-size: 10.0pt; font-family: Calibri; mso-ascii-font-family: Calibri; mso-fareast-font-family: \u5b8b\u4f53; mso-bidi-font-family: \u5b8b\u4f53; font-variant: normal; color: black; text-transform: none; mso-font-kerning: 12.0pt; language: zh-CN; font-weight: normal; font-style: normal; vertical-align: baseline; mso-text-raise: 0%; mso-style-textoutline-type: none; mso-style-textfill-type: solid; mso-style-textfill-fill-color: black; mso-style-textfill-fill-alpha: 100.0%;\">\u00a0<\/span> <span style=\"font-size: 10.0pt; font-family: Calibri; mso-ascii-font-family: Calibri; mso-fareast-font-family: \u5b8b\u4f53; mso-bidi-font-family: \u5b8b\u4f53; font-variant: normal; color: black; text-transform: none; mso-font-kerning: 12.0pt; language: zh-CN; font-weight: normal; font-style: normal; vertical-align: baseline; mso-text-raise: 0%; mso-style-textoutline-type: none; mso-style-textfill-type: solid; mso-style-textfill-fill-color: black; mso-style-textfill-fill-alpha: 100.0%;\">\u00a0 \u00a0 \u00a0\u6211\u4eec\u6d4b\u8bd5\u4e86\u4e0d\u540c\u7684<\/span><span style=\"font-size: 10.0pt; font-family: Calibri; mso-ascii-font-family: Calibri; mso-fareast-font-family: \u5b8b\u4f53; mso-bidi-font-family: \u5b8b\u4f53; font-variant: normal; color: black; text-transform: none; mso-font-kerning: 12.0pt; language: en-US; font-weight: normal; font-style: normal; vertical-align: baseline; mso-text-raise: 0%; mso-style-textoutline-type: none; mso-style-textfill-type: solid; mso-style-textfill-fill-color: black; mso-style-textfill-fill-alpha: 100.0%;\">NK<\/span><span style=\"font-size: 10.0pt; font-family: \u5b8b\u4f53; mso-ascii-font-family: Calibri; mso-fareast-font-family: \u5b8b\u4f53; mso-bidi-font-family: \u5b8b\u4f53; font-variant: normal; color: black; text-transform: none; mso-font-kerning: 12.0pt; language: zh-CN; font-weight: normal; font-style: normal; vertical-align: baseline; mso-text-raise: 0%; mso-style-textoutline-type: none; mso-style-textfill-type: solid; mso-style-textfill-fill-color: black; mso-style-textfill-fill-alpha: 100.0%;\">\u7ec6\u80de\u523a\u6fc0\u57f9\u517b\u65b9\u6848\uff0c<\/span><span style=\"font-size: 10.0pt; font-family: \u5b8b\u4f53; mso-ascii-font-family: Verdana; mso-fareast-font-family: \u5b8b\u4f53; mso-bidi-font-family: \u5b8b\u4f53; color: black; mso-font-kerning: 12.0pt; language: zh-CN; mso-style-textfill-type: solid; mso-style-textfill-fill-color: black; mso-style-textfill-fill-alpha: 100.0%;\">\u76ee\u524d\u8868\u73b0<\/span><span style=\"font-size: 10.0pt; font-family: \u5b8b\u4f53; mso-ascii-font-family: Verdana; mso-fareast-font-family: \u5b8b\u4f53; mso-bidi-font-family: \u5b8b\u4f53; color: black; mso-font-kerning: 12.0pt; language: zh-CN; mso-style-textfill-type: solid; mso-style-textfill-fill-color: black; mso-style-textfill-fill-alpha: 100.0%;\">\u6bd4\u8f83\u597d\u7684<\/span><span style=\"font-size: 10.0pt; font-family: \u5b8b\u4f53; mso-ascii-font-family: Verdana; mso-fareast-font-family: \u5b8b\u4f53; mso-bidi-font-family: \u5b8b\u4f53; color: black; mso-font-kerning: 12.0pt; language: zh-CN; mso-style-textfill-type: solid; mso-style-textfill-fill-color: black; mso-style-textfill-fill-alpha: 100.0%;\">\u662f\u52a0<\/span><span style=\"font-size: 10.0pt; font-family: Verdana; mso-ascii-font-family: Verdana; mso-fareast-font-family: \u5b8b\u4f53; mso-bidi-font-family: \u5b8b\u4f53; color: black; mso-font-kerning: 12.0pt; language: en-US; mso-style-textfill-type: solid; mso-style-textfill-fill-color: black; mso-style-textfill-fill-alpha: 100.0%;\">Feeder cell<\/span><span style=\"font-size: 10.0pt; font-family: \u5b8b\u4f53; mso-ascii-font-family: Verdana; mso-fareast-font-family: \u5b8b\u4f53; mso-bidi-font-family: \u5b8b\u4f53; color: black; mso-font-kerning: 12.0pt; language: zh-CN; mso-style-textfill-type: solid; mso-style-textfill-fill-color: black; mso-style-textfill-fill-alpha: 100.0%;\">\u57f9\u517b\uff0c<\/span><span style=\"font-size: 10.0pt; font-family: \u5b8b\u4f53; mso-ascii-font-family: Verdana; mso-fareast-font-family: \u5b8b\u4f53; mso-bidi-font-family: \u5b8b\u4f53; color: black; mso-font-kerning: 12.0pt; language: zh-CN; mso-style-textfill-type: solid; mso-style-textfill-fill-color: black; mso-style-textfill-fill-alpha: 100.0%;\">\u6bd4\u6db2\u4f53\u57f9\u517b\u57fa\u7684\u597d\u4e00\u4e9b<\/span><span style=\"font-size: 10.0pt; font-family: \u5b8b\u4f53; mso-ascii-font-family: Verdana; mso-fareast-font-family: \u5b8b\u4f53; mso-bidi-font-family: \u5b8b\u4f53; color: black; mso-font-kerning: 12.0pt; language: zh-CN; mso-style-textfill-type: solid; mso-style-textfill-fill-color: black; mso-style-textfill-fill-alpha: 100.0%;\">\u3002\u523a\u6fc0\u57f9\u517b<\/span><span style=\"font-size: 10.0pt; font-family: Verdana; mso-ascii-font-family: Verdana; mso-fareast-font-family: \u5b8b\u4f53; mso-bidi-font-family: \u5b8b\u4f53; color: black; mso-font-kerning: 12.0pt; language: en-US; mso-style-textfill-type: solid; mso-style-textfill-fill-color: black; mso-style-textfill-fill-alpha: 100.0%;\">6<\/span><span style=\"font-size: 10.0pt; font-family: \u5b8b\u4f53; mso-ascii-font-family: Verdana; mso-fareast-font-family: \u5b8b\u4f53; mso-bidi-font-family: \u5b8b\u4f53; color: black; mso-font-kerning: 12.0pt; language: zh-CN; mso-style-textfill-type: solid; mso-style-textfill-fill-color: black; mso-style-textfill-fill-alpha: 100.0%;\">\u5929\u7684<\/span><span style=\"font-size: 10.0pt; font-family: Verdana; mso-ascii-font-family: Verdana; mso-fareast-font-family: \u5b8b\u4f53; mso-bidi-font-family: \u5b8b\u4f53; color: black; mso-font-kerning: 12.0pt; language: en-US; mso-style-textfill-type: solid; mso-style-textfill-fill-color: black; mso-style-textfill-fill-alpha: 100.0%;\">NK<\/span><span style=\"font-size: 10.0pt; font-family: \u5b8b\u4f53; mso-ascii-font-family: Verdana; mso-fareast-font-family: \u5b8b\u4f53; mso-bidi-font-family: \u5b8b\u4f53; color: black; mso-font-kerning: 12.0pt; language: zh-CN; mso-style-textfill-type: solid; mso-style-textfill-fill-color: black; mso-style-textfill-fill-alpha: 100.0%;\">\u7ec6\u80de\u7535\u8f6c<\/span><span style=\"font-size: 10.0pt; font-family: Verdana; mso-ascii-font-family: Verdana; mso-fareast-font-family: \u5b8b\u4f53; mso-bidi-font-family: \u5b8b\u4f53; color: black; mso-font-kerning: 12.0pt; language: en-US; mso-style-textfill-type: solid; mso-style-textfill-fill-color: black; mso-style-textfill-fill-alpha: 100.0%;\">GFP<\/span><span style=\"font-size: 10.0pt; font-family: \u5b8b\u4f53; mso-ascii-font-family: Verdana; mso-fareast-font-family: \u5b8b\u4f53; mso-bidi-font-family: \u5b8b\u4f53; color: black; mso-font-kerning: 12.0pt; language: zh-CN; mso-style-textfill-type: solid; mso-style-textfill-fill-color: black; mso-style-textfill-fill-alpha: 100.0%;\">\u8f6c\u5ea7\u5b50\u540e\u518d\u57f9\u517b<\/span><span style=\"font-size: 10.0pt; font-family: Verdana; mso-ascii-font-family: Verdana; mso-fareast-font-family: \u5b8b\u4f53; mso-bidi-font-family: \u5b8b\u4f53; color: black; mso-font-kerning: 12.0pt; language: en-US; mso-style-textfill-type: solid; mso-style-textfill-fill-color: black; mso-style-textfill-fill-alpha: 100.0%;\">8<\/span><span style=\"font-size: 10.0pt; font-family: \u5b8b\u4f53; mso-ascii-font-family: Verdana; mso-fareast-font-family: \u5b8b\u4f53; mso-bidi-font-family: \u5b8b\u4f53; color: black; mso-font-kerning: 12.0pt; language: zh-CN; mso-style-textfill-type: solid; mso-style-textfill-fill-color: black; mso-style-textfill-fill-alpha: 100.0%;\">\u5929\uff0c<\/span><span style=\"font-size: 10.0pt; font-family: Verdana; mso-ascii-font-family: Verdana; mso-fareast-font-family: \u5b8b\u4f53; mso-bidi-font-family: \u5b8b\u4f53; color: black; mso-font-kerning: 12.0pt; language: en-US; mso-style-textfill-type: solid; mso-style-textfill-fill-color: black; mso-style-textfill-fill-alpha: 100.0%;\">GFP<\/span><span style=\"font-size: 10.0pt; font-family: \u5b8b\u4f53; mso-ascii-font-family: Verdana; mso-fareast-font-family: \u5b8b\u4f53; mso-bidi-font-family: \u5b8b\u4f53; color: black; mso-font-kerning: 12.0pt; language: zh-CN; mso-style-textfill-type: solid; mso-style-textfill-fill-color: black; mso-style-textfill-fill-alpha: 100.0%;\">\u9633\u6027<\/span><span style=\"font-size: 10.0pt; font-family: \u5b8b\u4f53; mso-ascii-font-family: Verdana; mso-fareast-font-family: \u5b8b\u4f53; mso-bidi-font-family: \u5b8b\u4f53; color: black; mso-font-kerning: 12.0pt; language: zh-CN; mso-style-textfill-type: solid; mso-style-textfill-fill-color: black; mso-style-textfill-fill-alpha: 100.0%;\">\u7387\u53ef\u4ee5\u7ef4\u6301<\/span><span style=\"font-size: 10.0pt; font-family: \u5b8b\u4f53; mso-ascii-font-family: Verdana; mso-fareast-font-family: \u5b8b\u4f53; mso-bidi-font-family: \u5b8b\u4f53; color: black; mso-font-kerning: 12.0pt; language: zh-CN; mso-style-textfill-type: solid; mso-style-textfill-fill-color: black; mso-style-textfill-fill-alpha: 100.0%;\">\u5728<\/span><span style=\"font-size: 10.0pt; font-family: Verdana; mso-ascii-font-family: Verdana; mso-fareast-font-family: \u5b8b\u4f53; mso-bidi-font-family: \u5b8b\u4f53; color: black; mso-font-kerning: 12.0pt; language: en-US; mso-style-textfill-type: solid; mso-style-textfill-fill-color: black; mso-style-textfill-fill-alpha: 100.0%;\">50<\/span><span style=\"font-size: 10.0pt; font-family: Verdana; mso-ascii-font-family: Verdana; mso-fareast-font-family: \u5b8b\u4f53; mso-bidi-font-family: \u5b8b\u4f53; color: black; mso-font-kerning: 12.0pt; language: en-US; mso-style-textfill-type: solid; mso-style-textfill-fill-color: black; mso-style-textfill-fill-alpha: 100.0%;\">%<\/span><span style=\"font-size: 10.0pt; font-family: \u5b8b\u4f53; mso-ascii-font-family: Verdana; mso-fareast-font-family: \u5b8b\u4f53; mso-bidi-font-family: \u5b8b\u4f53; color: black; mso-font-kerning: 12.0pt; language: zh-CN; mso-style-textfill-type: solid; mso-style-textfill-fill-color: black; mso-style-textfill-fill-alpha: 100.0%;\">\u3002\u4e0d\u540c\u523a\u6fc0\u57f9\u517b\u6761\u4ef6\u7684<\/span><span style=\"font-size: 10.0pt; font-family: Verdana; mso-ascii-font-family: Verdana; mso-fareast-font-family: \u5b8b\u4f53; mso-bidi-font-family: \u5b8b\u4f53; color: black; mso-font-kerning: 12.0pt; language: en-US; mso-style-textfill-type: solid; mso-style-textfill-fill-color: black; mso-style-textfill-fill-alpha: 100.0%;\">NK<\/span><span style=\"font-size: 10.0pt; font-family: \u5b8b\u4f53; mso-ascii-font-family: Verdana; mso-fareast-font-family: \u5b8b\u4f53; mso-bidi-font-family: \u5b8b\u4f53; color: black; mso-font-kerning: 12.0pt; language: zh-CN; mso-style-textfill-type: solid; mso-style-textfill-fill-color: black; mso-style-textfill-fill-alpha: 100.0%;\">\u7ec6\u80de\uff0c<\/span><span style=\"font-size: 10.0pt; font-family: \u5b8b\u4f53; mso-ascii-font-family: Verdana; mso-fareast-font-family: \u5b8b\u4f53; mso-bidi-font-family: \u5b8b\u4f53; font-variant: normal; color: black; text-transform: none; mso-font-kerning: 12.0pt; language: zh-CN; font-weight: normal; font-style: normal; vertical-align: baseline; mso-text-raise: 0%; mso-style-textoutline-type: none; mso-style-textfill-type: solid; mso-style-textfill-fill-color: black; mso-style-textfill-fill-alpha: 100.0%;\">\u4e3a\u4e86\u8fbe\u5230\u9ad8\u6548\u7387\u8f6c\u67d3\u5e76\u4e14\u4fdd\u6301\u7ec6\u80de\u826f\u597d\u7684\u751f\u957f\u6d3b\u6027\uff0c\u7ec6\u80de\u523a\u6fc0\u65f6\u95f4\u4ee5\u53ca\u7535\u8f6c\u7684\u7535\u538b\u90fd\u9700\u8981\u7ecf\u8fc7\u4ed4\u7ec6\u6d4b\u8bd5\u3002<\/span><\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-d550083 elementor-widget elementor-widget-image\" data-id=\"d550083\" data-element_type=\"widget\" data-widget_type=\"image.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<img fetchpriority=\"high\" decoding=\"async\" width=\"400\" height=\"198\" src=\"https:\/\/www.celetrix.com\/wp-content\/uploads\/2022\/12\/NK\u53c2\u6570\u63a8\u8350\u56fe-1-e1670899687801.png\" class=\"attachment-full size-full wp-image-766\"  srcset=\"https:\/\/www.celetrix.com\/wp-content\/uploads\/2022\/12\/NK\u53c2\u6570\u63a8\u8350\u56fe-1-e1670899687801.png 400w, https:\/\/www.celetrix.com\/wp-content\/uploads\/2022\/12\/NK\u53c2\u6570\u63a8\u8350\u56fe-1-e1670899687801-300x149.png 300w, https:\/\/www.celetrix.com\/wp-content\/uploads\/2022\/12\/NK\u53c2\u6570\u63a8\u8350\u56fe-1-e1670899687801-18x9.png 18w\" sizes=\"(max-width: 400px) 100vw, 400px\" title=\"Human Cultured NK cells\u63d2\u56fe\" alt=\"Human Cultured NK cells\u63d2\u56fe\" \/>\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-ca9c1d1 elementor-widget elementor-widget-image\" data-id=\"ca9c1d1\" data-element_type=\"widget\" data-widget_type=\"image.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<img decoding=\"async\" width=\"400\" height=\"196\" src=\"https:\/\/www.celetrix.com\/wp-content\/uploads\/2022\/12\/NK\u53c2\u6570\u63a8\u8350\u56fe-2-e1670899733187.png\" class=\"attachment-full size-full wp-image-767\"  srcset=\"https:\/\/www.celetrix.com\/wp-content\/uploads\/2022\/12\/NK\u53c2\u6570\u63a8\u8350\u56fe-2-e1670899733187.png 400w, https:\/\/www.celetrix.com\/wp-content\/uploads\/2022\/12\/NK\u53c2\u6570\u63a8\u8350\u56fe-2-e1670899733187-300x147.png 300w, https:\/\/www.celetrix.com\/wp-content\/uploads\/2022\/12\/NK\u53c2\u6570\u63a8\u8350\u56fe-2-e1670899733187-18x9.png 18w\" sizes=\"(max-width: 400px) 100vw, 400px\" title=\"Human Cultured NK cells\u63d2\u56fe1\" alt=\"Human Cultured NK cells\u63d2\u56fe1\" \/>\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-ba0fdc4 elementor-widget elementor-widget-text-editor\" data-id=\"ba0fdc4\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p style=\"text-align: center;\">\u523a\u6fc0\u57f9\u517b6\u5929NK\u7ec6\u80de\u7535\u8f6cGFP\u8f6c\u5ea7\u5b50\u540e48\u5c0f\u65f6GFP\u9633\u6027\u738780%\uff0c\u518d\u63a5\u7740\u57f9\u517b8\u5929GFP\u9633\u6027\u7387\u7ef4\u6301\u572850%\u3002<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<section class=\"elementor-section elementor-inner-section elementor-element elementor-element-3c5cffb elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"3c5cffb\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-50 elementor-inner-column elementor-element elementor-element-34609f8\" data-id=\"34609f8\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-4a8977f elementor-widget elementor-widget-text-editor\" data-id=\"4a8977f\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<h5 style=\"text-align: center;\"><strong>Human Cultured NK cells in 20ul tubes<\/strong><\/h5><p>\u00a0 Cell Number\uff1a1-2*10^6 cells<\/p><p>\u00a0 Plasmid\uff1a2ug<\/p><p>\u00a0 E buffer\uff1aCeletrix buffer<\/p><p>\u00a0 Suggested parameter\uff1aCell Line Mode\uff0c480V-540V<\/p><p>\u00a0 Culture medium: \u65e0\u6297\u751f\u7d20NK\u7ec6\u80de\u57f9\u517b\u57fa<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t<div class=\"elementor-column elementor-col-50 elementor-inner-column elementor-element elementor-element-b8dc9f6\" data-id=\"b8dc9f6\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-15ad8a4 elementor-widget elementor-widget-text-editor\" data-id=\"15ad8a4\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<h5 style=\"text-align: center;\"><strong>Human Cultured NK cells in 100ul tubes<\/strong><\/h5>\n<p>&nbsp; Cell Number\uff1a5-10*10^6 cells<\/p>\n<p>&nbsp; Plasmid\uff1a10ug<\/p>\n<p>&nbsp; E buffer\uff1aCeletrix buffer<\/p>\n<p>&nbsp; Suggested parameter\uff1aCell Line Mode\uff0c480V-540V<\/p>\n<p>&nbsp; Culture medium: \u65e0\u6297\u751f\u7d20NK\u7ec6\u80de\u57f9\u517b\u57fa<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t<div class=\"elementor-column elementor-col-50 elementor-top-column elementor-element elementor-element-12880d5\" data-id=\"12880d5\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-d420a09 elementor-nav-menu__align-start elementor-nav-menu--dropdown-none elementor-widget elementor-widget-nav-menu\" data-id=\"d420a09\" data-element_type=\"widget\" data-settings=\"{&quot;layout&quot;:&quot;vertical&quot;,&quot;submenu_icon&quot;:{&quot;value&quot;:&quot;&lt;i class=\\&quot;fas fa-caret-down\\&quot; aria-hidden=\\&quot;true\\&quot;&gt;&lt;\\\/i&gt;&quot;,&quot;library&quot;:&quot;fa-solid&quot;}}\" data-widget_type=\"nav-menu.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t<nav aria-label=\"Menu\" class=\"elementor-nav-menu--main elementor-nav-menu__container elementor-nav-menu--layout-vertical e--pointer-background e--animation-fade\">\n\t\t\t\t<ul id=\"menu-1-d420a09\" class=\"elementor-nav-menu sm-vertical\"><li class=\"menu-item menu-item-type-post_type menu-item-object-page menu-item-105\"><a href=\"https:\/\/www.celetrix.com\/zh\/manuals-videos\/\" class=\"elementor-item\">Manuals &#038; Videos<\/a><\/li>\n<li class=\"menu-item menu-item-type-post_type menu-item-object-page menu-item-107\"><a href=\"https:\/\/www.celetrix.com\/zh\/recommend-parameters\/\" class=\"elementor-item\">Recommend Parameters<\/a><\/li>\n<li class=\"menu-item menu-item-type-post_type menu-item-object-page menu-item-106\"><a href=\"https:\/\/www.celetrix.com\/zh\/optimization-strategy\/\" class=\"elementor-item\">Optimization Strategy<\/a><\/li>\n<li class=\"menu-item menu-item-type-post_type menu-item-object-page menu-item-104\"><a href=\"https:\/\/www.celetrix.com\/zh\/experimental-overview\/\" class=\"elementor-item\">Experimental Overview<\/a><\/li>\n<li class=\"menu-item menu-item-type-post_type menu-item-object-page menu-item-108\"><a href=\"https:\/\/www.celetrix.com\/zh\/special-cells\/\" class=\"elementor-item\">Special Cells<\/a><\/li>\n<\/ul>\t\t\t<\/nav>\n\t\t\t\t\t\t<nav class=\"elementor-nav-menu--dropdown elementor-nav-menu__container\" aria-hidden=\"true\">\n\t\t\t\t<ul id=\"menu-2-d420a09\" class=\"elementor-nav-menu sm-vertical\"><li class=\"menu-item menu-item-type-post_type menu-item-object-page menu-item-105\"><a href=\"https:\/\/www.celetrix.com\/zh\/manuals-videos\/\" class=\"elementor-item\" tabindex=\"-1\">Manuals &#038; Videos<\/a><\/li>\n<li class=\"menu-item menu-item-type-post_type menu-item-object-page menu-item-107\"><a href=\"https:\/\/www.celetrix.com\/zh\/recommend-parameters\/\" class=\"elementor-item\" tabindex=\"-1\">Recommend Parameters<\/a><\/li>\n<li class=\"menu-item menu-item-type-post_type menu-item-object-page menu-item-106\"><a href=\"https:\/\/www.celetrix.com\/zh\/optimization-strategy\/\" class=\"elementor-item\" tabindex=\"-1\">Optimization Strategy<\/a><\/li>\n<li class=\"menu-item menu-item-type-post_type menu-item-object-page menu-item-104\"><a href=\"https:\/\/www.celetrix.com\/zh\/experimental-overview\/\" class=\"elementor-item\" tabindex=\"-1\">Experimental Overview<\/a><\/li>\n<li class=\"menu-item menu-item-type-post_type menu-item-object-page menu-item-108\"><a href=\"https:\/\/www.celetrix.com\/zh\/special-cells\/\" class=\"elementor-item\" tabindex=\"-1\">Special Cells<\/a><\/li>\n<\/ul>\t\t\t<\/nav>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<\/div>","protected":false},"excerpt":{"rendered":"<p>Electroporation by Celetrix Page Human Cultured NK cells \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u5728\u7535\u8f6c\u6761\u4ef6\u4e0a\uff0c\u539f\u4ee3\u7eaf\u5316\u7684NK\u7ec6\u80de\u548cPBMC\u7535\u8f6c\u6bd4\u8f83\u7c7b\u4f3c\u3002\u8fd9\u4e9b\u7ec6\u80de\u5728\u672a\u523a\u6fc0\u57f9\u517b\u65f6\uff0c\u76f4\u5f84\u6bd4\u8f83\u5c0f\uff0c\u53ea\u67097-8um\uff0c\u7535\u8f6c\u9700\u8981\u7684\u7535\u538b\u6bd4\u666e\u901a\u7ec6\u80de\u7cfb\u9ad8\u5f88\u591a\uff0c\u53ef\u4ee5\u76f4\u63a5\u53c2\u7167PBMC\u7684\u7535\u8f6c\u6761\u4ef6\u8fdb\u884c\u64cd\u4f5c\u3002 \u00a0 \u00a0\u00a0\u00a0\u00a0\u00a0\u00a0 \u523a\u6fc0\u57f9\u517b\u7684NK\u7684\u7ec6\u80de\u5728\u751f\u957f\u8fc7\u7a0b\u4e2d\u4f53\u79ef\u4f1a\u53d8\u5927\u3002\u56e0\u4e3a\u5927\u4f53\u79ef\u7ec6\u80de\u9700\u8981\u7535\u538b\u8f83\u4f4e\uff0c\u6240\u4ee5\u523a\u6fc0\u57f9\u517b\u7684NK\u7ec6\u80de\u7535\u8f6c\u9700\u8981\u7684\u7535\u538b\u4f1a\u6bd4\u539f\u4ee3NK\u7ec6\u80de\u4f4e\u5f88\u591a\uff0c\u548c\u666e\u901a\u7ec6\u80de\u7cfb\u7535\u8f6c\u7535\u538b\u7c7b\u4f3c\uff0c\u4f7f\u7528Cell line\u6a21\u5f0f\u7535\u8f6c\u5373\u53ef\u3002 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0\u6211\u4eec\u6d4b\u8bd5\u4e86\u4e0d\u540c\u7684NK\u7ec6\u80de\u523a\u6fc0\u57f9\u517b\u65b9\u6848\uff0c\u76ee\u524d\u8868\u73b0\u6bd4\u8f83\u597d\u7684\u662f\u52a0Feeder cell\u57f9\u517b\uff0c\u6bd4\u6db2\u4f53\u57f9\u517b\u57fa\u7684\u597d\u4e00\u4e9b\u3002\u523a\u6fc0\u57f9\u517b6\u5929\u7684NK\u7ec6\u80de\u7535\u8f6cGFP\u8f6c\u5ea7\u5b50\u540e\u518d\u57f9\u517b8\u5929\uff0cGFP\u9633\u6027\u7387\u53ef\u4ee5\u7ef4\u6301\u572850%\u3002\u4e0d\u540c\u523a\u6fc0\u57f9\u517b\u6761\u4ef6\u7684NK\u7ec6\u80de\uff0c\u4e3a\u4e86\u8fbe\u5230\u9ad8\u6548\u7387\u8f6c\u67d3\u5e76\u4e14\u4fdd\u6301\u7ec6\u80de\u826f\u597d\u7684\u751f\u957f\u6d3b\u6027\uff0c\u7ec6\u80de\u523a\u6fc0\u65f6\u95f4\u4ee5\u53ca\u7535\u8f6c\u7684\u7535\u538b\u90fd\u9700\u8981\u7ecf\u8fc7\u4ed4\u7ec6\u6d4b\u8bd5\u3002 \u523a\u6fc0\u57f9\u517b6\u5929NK\u7ec6\u80de\u7535\u8f6cGFP\u8f6c\u5ea7\u5b50\u540e48\u5c0f\u65f6GFP\u9633\u6027\u738780%\uff0c\u518d\u63a5\u7740\u57f9\u517b8\u5929GFP\u9633\u6027\u7387\u7ef4\u6301\u572850%\u3002 Human Cultured NK cells in 20ul tubes \u00a0 Cell Number\uff1a1-2*10^6 cells \u00a0 Plasmid\uff1a2ug \u00a0 E buffer\uff1aCeletrix buffer \u00a0 Suggested parameter\uff1aCell Line Mode\uff0c480V-540V \u00a0 Culture medium: \u65e0\u6297\u751f\u7d20NK\u7ec6\u80de\u57f9\u517b\u57fa Human Cultured [&hellip;]<\/p>","protected":false},"author":1,"featured_media":0,"parent":51,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"elementor_header_footer","meta":{"om_disable_all_campaigns":false,"_uf_show_specific_survey":0,"_uf_disable_surveys":false,"footnotes":""},"class_list":["post-764","page","type-page","status-publish","hentry"],"aioseo_notices":[],"_links":{"self":[{"href":"https:\/\/www.celetrix.com\/zh\/wp-json\/wp\/v2\/pages\/764","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.celetrix.com\/zh\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/www.celetrix.com\/zh\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/www.celetrix.com\/zh\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.celetrix.com\/zh\/wp-json\/wp\/v2\/comments?post=764"}],"version-history":[{"count":16,"href":"https:\/\/www.celetrix.com\/zh\/wp-json\/wp\/v2\/pages\/764\/revisions"}],"predecessor-version":[{"id":1285,"href":"https:\/\/www.celetrix.com\/zh\/wp-json\/wp\/v2\/pages\/764\/revisions\/1285"}],"up":[{"embeddable":true,"href":"https:\/\/www.celetrix.com\/zh\/wp-json\/wp\/v2\/pages\/51"}],"wp:attachment":[{"href":"https:\/\/www.celetrix.com\/zh\/wp-json\/wp\/v2\/media?parent=764"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}