{"id":53,"date":"2021-10-28T08:17:20","date_gmt":"2021-10-28T08:17:20","guid":{"rendered":"https:\/\/celetrix.com\/celetrix\/?page_id=53"},"modified":"2026-10-26T06:48:22","modified_gmt":"2026-10-26T06:48:22","slug":"optimization-strategy","status":"publish","type":"page","link":"https:\/\/www.celetrix.com\/zh\/optimization-strategy\/","title":{"rendered":"Optimization Strategy"},"content":{"rendered":"\t\t<div data-elementor-type=\"wp-page\" data-elementor-id=\"53\" class=\"elementor elementor-53\" data-elementor-post-type=\"page\">\n\t\t\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-3976d66 elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"3976d66\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-50 elementor-top-column elementor-element elementor-element-30b36ff\" data-id=\"30b36ff\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-22fdf5c elementor-widget elementor-widget-bdt-breadcrumbs\" data-id=\"22fdf5c\" data-element_type=\"widget\" data-widget_type=\"bdt-breadcrumbs.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<div class='bdt-breadcrumbs-wrapper'><ul class=\"bdt-breadcrumb breadcrumb-align-left  \"><li><a href=\"https:\/\/www.celetrix.com\/zh\/\"><span class=\"bdt-breadcrumbs-home-icon\"> <i aria-hidden=\"true\" class=\"fas fa-home\"><\/i> <\/span>Electroporation by Celetrix<\/a><\/li>\n<li><span> \u9875\u9762<\/span><\/li>\n<\/ul><\/div>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-ef8367a elementor-widget elementor-widget-heading\" data-id=\"ef8367a\" data-element_type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h1 class=\"elementor-heading-title elementor-size-default\">How to Optimize Electroporation Efficiency<\/h1>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-039860f elementor-widget elementor-widget-text-editor\" data-id=\"039860f\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p>Optimizing Celetrix electroporation is easy. Start from our <a href=\"\/recommend-parameters\/\">recommended parameters<\/a>, run a quick GFP plasmid test, and fine-tune the voltage for your cell density, temperature and payload.<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-2bfc0c7 elementor-widget elementor-widget-heading\" data-id=\"2bfc0c7\" data-element_type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h2 class=\"elementor-heading-title elementor-size-default\">Quick Optimization Workflow<\/h2>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-a94bb57 elementor-widget elementor-widget-text-editor\" data-id=\"a94bb57\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<ol><li>Start from the recommended voltage for your cell type. For unlisted cells, use a cell type of similar size.<\/li><li>Test 3 voltages, 20 V apart, with a GFP plasmid.<\/li><li>Keep test conditions the same as your real experiment: cell density, culture and DNA amount.<\/li><li>Check GFP under a microscope 1\u20133 h after electroporation; expression is stronger after overnight culture.<\/li><li>Pick the best voltage and keep cell density fixed from then on.<\/li><\/ol>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-b4a1878 elementor-widget elementor-widget-heading\" data-id=\"b4a1878\" data-element_type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h2 class=\"elementor-heading-title elementor-size-default\">Key Factors That Affect Electroporation<\/h2>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-c98606b elementor-widget elementor-widget-text-editor\" data-id=\"c98606b\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<table class=\"cx-scale-table\" style=\"width:100%;border-collapse:collapse;table-layout:fixed;font-family:Montserrat,sans-serif;font-size:16px;\"><tbody><tr><td style=\"text-align:left;vertical-align:middle;padding:14px 16px;border-bottom:1px solid #dee2e6;overflow-wrap:anywhere;width:26%;font-weight:600;\">Cell health<\/td><td style=\"text-align:left;vertical-align:middle;padding:14px 16px;border-bottom:1px solid #dee2e6;overflow-wrap:anywhere;\">Culture cells to 50\u201370% of their maximum density before electroporation, and keep this consistent.<\/td><\/tr><tr><td style=\"text-align:left;vertical-align:middle;padding:14px 16px;border-bottom:1px solid #dee2e6;overflow-wrap:anywhere;width:26%;font-weight:600;\">Cell density<\/td><td style=\"text-align:left;vertical-align:middle;padding:14px 16px;border-bottom:1px solid #dee2e6;overflow-wrap:anywhere;\">Cell lines: 0.5\u20132\u00d710<sup>6<\/sup> per 20 \u00b5l (typically 1\u00d710<sup>6<\/sup>) or 2.5\u201310\u00d710<sup>6<\/sup> per 100 \u00b5l. PBMC: 1\u20135\u00d710<sup>6<\/sup> per 20 \u00b5l. Higher density needs slightly higher voltage.<\/td><\/tr><tr><td style=\"text-align:left;vertical-align:middle;padding:14px 16px;border-bottom:1px solid #dee2e6;overflow-wrap:anywhere;width:26%;font-weight:600;\">Single cells<\/td><td style=\"text-align:left;vertical-align:middle;padding:14px 16px;border-bottom:1px solid #dee2e6;overflow-wrap:anywhere;\">Avoid clumps: clumped cells transfect poorly. Do not over-digest adherent cells.<\/td><\/tr><tr><td style=\"text-align:left;vertical-align:middle;padding:14px 16px;border-bottom:1px solid #dee2e6;overflow-wrap:anywhere;width:26%;font-weight:600;\">Temperature<\/td><td style=\"text-align:left;vertical-align:middle;padding:14px 16px;border-bottom:1px solid #dee2e6;overflow-wrap:anywhere;\">Room temperature (about 23 \u00b0C) works best. At 4 \u00b0C, voltage needs to be 10\u201320% higher. Adjust voltage if your lab is very warm or cold.<\/td><\/tr><tr><td style=\"text-align:left;vertical-align:middle;padding:14px 16px;border-bottom:1px solid #dee2e6;overflow-wrap:anywhere;width:26%;font-weight:600;\">DNA quality<\/td><td style=\"text-align:left;vertical-align:middle;padding:14px 16px;border-bottom:1px solid #dee2e6;overflow-wrap:anywhere;\">Use high-purity plasmid at 50\u2013200 \u00b5g\/ml. More DNA raises efficiency but also toxicity, so lower the voltage slightly.<\/td><\/tr><tr><td style=\"text-align:left;vertical-align:middle;padding:14px 16px;border-bottom:1px solid #dee2e6;overflow-wrap:anywhere;width:26%;font-weight:600;\">Payload<\/td><td style=\"text-align:left;vertical-align:middle;padding:14px 16px;border-bottom:1px solid #dee2e6;overflow-wrap:anywhere;\">Large plasmid &lt; small plasmid &lt; Cas9 RNP \/ siRNA (voltage needed).<\/td><\/tr><tr><td style=\"text-align:left;vertical-align:middle;padding:14px 16px;border-bottom:1px solid #dee2e6;overflow-wrap:anywhere;width:26%;font-weight:600;\">Recovery<\/td><td style=\"text-align:left;vertical-align:middle;padding:14px 16px;border-bottom:1px solid #dee2e6;overflow-wrap:anywhere;\">Recover in antibiotic-free medium; antibiotics can be added from day 2. After high voltage, plate cells at higher density.<\/td><\/tr><\/tbody><\/table>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-c0ca1ae elementor-widget elementor-widget-heading\" data-id=\"c0ca1ae\" data-element_type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h2 class=\"elementor-heading-title elementor-size-default\">Voltage Conversion Between Tube Sizes<\/h2>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-1ca8ce5 elementor-widget elementor-widget-text-editor\" data-id=\"1ca8ce5\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p style=\"margin:0 0 10px;\">Pulse settings stay the same; only the voltage changes. Example based on 500 V in a 20 \u00b5l tube:<\/p><table class=\"cx-scale-table\" style=\"width:100%;border-collapse:collapse;table-layout:fixed;font-family:Montserrat,sans-serif;font-size:16px;\"><tbody><tr><td style=\"text-align:left;vertical-align:middle;padding:14px 16px;border-bottom:1px solid #dee2e6;overflow-wrap:anywhere;width:26%;font-weight:600;\">20 \u00b5l<\/td><td style=\"text-align:left;vertical-align:middle;padding:14px 16px;border-bottom:1px solid #dee2e6;overflow-wrap:anywhere;\">500 V (base)<\/td><\/tr><tr><td style=\"text-align:left;vertical-align:middle;padding:14px 16px;border-bottom:1px solid #dee2e6;overflow-wrap:anywhere;width:26%;font-weight:600;\">100 \u00b5l<\/td><td style=\"text-align:left;vertical-align:middle;padding:14px 16px;border-bottom:1px solid #dee2e6;overflow-wrap:anywhere;\">510 V (20 \u00b5l + 10 V)<\/td><\/tr><tr><td style=\"text-align:left;vertical-align:middle;padding:14px 16px;border-bottom:1px solid #dee2e6;overflow-wrap:anywhere;width:26%;font-weight:600;\">120 \u00b5l<\/td><td style=\"text-align:left;vertical-align:middle;padding:14px 16px;border-bottom:1px solid #dee2e6;overflow-wrap:anywhere;\">700 V (20 \u00b5l \u00d7 1.4)<\/td><\/tr><tr><td style=\"text-align:left;vertical-align:middle;padding:14px 16px;border-bottom:1px solid #dee2e6;overflow-wrap:anywhere;width:26%;font-weight:600;\">200 \u00b5l<\/td><td style=\"text-align:left;vertical-align:middle;padding:14px 16px;border-bottom:1px solid #dee2e6;overflow-wrap:anywhere;\">1200 V (20 \u00b5l \u00d7 2.4)<\/td><\/tr><tr><td style=\"text-align:left;vertical-align:middle;padding:14px 16px;border-bottom:1px solid #dee2e6;overflow-wrap:anywhere;width:26%;font-weight:600;\">600 \u00b5l<\/td><td style=\"text-align:left;vertical-align:middle;padding:14px 16px;border-bottom:1px solid #dee2e6;overflow-wrap:anywhere;\">800 V (20 \u00b5l \u00d7 1.6)<\/td><\/tr><tr><td style=\"text-align:left;vertical-align:middle;padding:14px 16px;border-bottom:1px solid #dee2e6;overflow-wrap:anywhere;width:26%;font-weight:600;\">1 ml<\/td><td style=\"text-align:left;vertical-align:middle;padding:14px 16px;border-bottom:1px solid #dee2e6;overflow-wrap:anywhere;\">1250 V (20 \u00b5l \u00d7 2.5)<\/td><\/tr><\/tbody><\/table><div style=\"background:#F5F8FB;border-left:4px solid #0078B5;padding:14px 18px;margin-top:16px;\"><p style=\"margin:0 0 6px;font-weight:700;color:#0078B5;\">Easy-to-miss details<\/p><ul style=\"margin:0;padding-left:18px;\"><li>Electroporate each sample right after loading. Do not load a whole batch first: cells settle and efficiency drops.<\/li><li>Bubbles larger than 1\/3 of the tube diameter: reopen the tube and reload.<\/li><li>\u201cLow Current\u201d alarm: check for large bubbles and reload the sample.<\/li><li>Re-test with GFP when you switch to a different tube size or change cell density a lot.<\/li><\/ul><\/div>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-b10b4bd elementor-widget elementor-widget-heading\" data-id=\"b10b4bd\" data-element_type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h2 class=\"elementor-heading-title elementor-size-default\">Electroporation Optimization FAQs<\/h2>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-831ce8d elementor-widget elementor-widget-accordion\" data-id=\"831ce8d\" data-element_type=\"widget\" data-widget_type=\"accordion.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t<div class=\"elementor-accordion\">\n\t\t\t\t\t\t\t<div class=\"elementor-accordion-item\">\n\t\t\t\t\t<div id=\"elementor-tab-title-1371\" class=\"elementor-tab-title\" data-tab=\"1\" role=\"button\" aria-controls=\"elementor-tab-content-1371\" aria-expanded=\"false\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t<span class=\"elementor-accordion-icon elementor-accordion-icon-left\" aria-hidden=\"true\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<span class=\"elementor-accordion-icon-closed\"><svg class=\"e-font-icon-svg e-fas-plus\" viewBox=\"0 0 448 512\" xmlns=\"http:\/\/www.w3.org\/2000\/svg\"><path d=\"M416 208H272V64c0-17.67-14.33-32-32-32h-32c-17.67 0-32 14.33-32 32v144H32c-17.67 0-32 14.33-32 32v32c0 17.67 14.33 32 32 32h144v144c0 17.67 14.33 32 32 32h32c17.67 0 32-14.33 32-32V304h144c17.67 0 32-14.33 32-32v-32c0-17.67-14.33-32-32-32z\"><\/path><\/svg><\/span>\n\t\t\t\t\t\t\t\t<span class=\"elementor-accordion-icon-opened\"><svg class=\"e-font-icon-svg e-fas-minus\" viewBox=\"0 0 448 512\" xmlns=\"http:\/\/www.w3.org\/2000\/svg\"><path d=\"M416 208H32c-17.67 0-32 14.33-32 32v32c0 17.67 14.33 32 32 32h384c17.67 0 32-14.33 32-32v-32c0-17.67-14.33-32-32-32z\"><\/path><\/svg><\/span>\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/span>\n\t\t\t\t\t\t\t\t\t\t\t\t<a class=\"elementor-accordion-title\" tabindex=\"0\">What is electroporation and how does it work?<\/a>\n\t\t\t\t\t<\/div>\n\t\t\t\t\t<div id=\"elementor-tab-content-1371\" class=\"elementor-tab-content elementor-clearfix\" data-tab=\"1\" role=\"region\" aria-labelledby=\"elementor-tab-title-1371\"><p>Electroporation uses a brief electric pulse to drive DNA, mRNA, siRNA, proteins or Cas9 RNP across the cell membrane. The voltage falls mostly on the cell membrane, so the inside of the cell is protected. Celetrix electroporators suspend cells in a protective Celetrix buffer to keep viability high.<\/p><\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t\t\t<div class=\"elementor-accordion-item\">\n\t\t\t\t\t<div id=\"elementor-tab-title-1372\" class=\"elementor-tab-title\" data-tab=\"2\" role=\"button\" aria-controls=\"elementor-tab-content-1372\" aria-expanded=\"false\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t<span class=\"elementor-accordion-icon elementor-accordion-icon-left\" aria-hidden=\"true\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<span class=\"elementor-accordion-icon-closed\"><svg class=\"e-font-icon-svg e-fas-plus\" viewBox=\"0 0 448 512\" xmlns=\"http:\/\/www.w3.org\/2000\/svg\"><path d=\"M416 208H272V64c0-17.67-14.33-32-32-32h-32c-17.67 0-32 14.33-32 32v144H32c-17.67 0-32 14.33-32 32v32c0 17.67 14.33 32 32 32h144v144c0 17.67 14.33 32 32 32h32c17.67 0 32-14.33 32-32V304h144c17.67 0 32-14.33 32-32v-32c0-17.67-14.33-32-32-32z\"><\/path><\/svg><\/span>\n\t\t\t\t\t\t\t\t<span class=\"elementor-accordion-icon-opened\"><svg class=\"e-font-icon-svg e-fas-minus\" viewBox=\"0 0 448 512\" xmlns=\"http:\/\/www.w3.org\/2000\/svg\"><path d=\"M416 208H32c-17.67 0-32 14.33-32 32v32c0 17.67 14.33 32 32 32h384c17.67 0 32-14.33 32-32v-32c0-17.67-14.33-32-32-32z\"><\/path><\/svg><\/span>\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/span>\n\t\t\t\t\t\t\t\t\t\t\t\t<a class=\"elementor-accordion-title\" tabindex=\"0\">How can I increase electroporation efficiency?<\/a>\n\t\t\t\t\t<\/div>\n\t\t\t\t\t<div id=\"elementor-tab-content-1372\" class=\"elementor-tab-content elementor-clearfix\" data-tab=\"2\" role=\"region\" aria-labelledby=\"elementor-tab-title-1372\"><p>With Celetrix electroporation, start from the recommended voltage for your cell type and test 3 voltages, 20 V apart, with a GFP plasmid. Use healthy, single cells and electroporate each sample right after loading the tube.<\/p><\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t\t\t<div class=\"elementor-accordion-item\">\n\t\t\t\t\t<div id=\"elementor-tab-title-1373\" class=\"elementor-tab-title\" data-tab=\"3\" role=\"button\" aria-controls=\"elementor-tab-content-1373\" aria-expanded=\"false\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t<span class=\"elementor-accordion-icon elementor-accordion-icon-left\" aria-hidden=\"true\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<span class=\"elementor-accordion-icon-closed\"><svg class=\"e-font-icon-svg e-fas-plus\" viewBox=\"0 0 448 512\" xmlns=\"http:\/\/www.w3.org\/2000\/svg\"><path d=\"M416 208H272V64c0-17.67-14.33-32-32-32h-32c-17.67 0-32 14.33-32 32v144H32c-17.67 0-32 14.33-32 32v32c0 17.67 14.33 32 32 32h144v144c0 17.67 14.33 32 32 32h32c17.67 0 32-14.33 32-32V304h144c17.67 0 32-14.33 32-32v-32c0-17.67-14.33-32-32-32z\"><\/path><\/svg><\/span>\n\t\t\t\t\t\t\t\t<span class=\"elementor-accordion-icon-opened\"><svg class=\"e-font-icon-svg e-fas-minus\" viewBox=\"0 0 448 512\" xmlns=\"http:\/\/www.w3.org\/2000\/svg\"><path d=\"M416 208H32c-17.67 0-32 14.33-32 32v32c0 17.67 14.33 32 32 32h384c17.67 0 32-14.33 32-32v-32c0-17.67-14.33-32-32-32z\"><\/path><\/svg><\/span>\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/span>\n\t\t\t\t\t\t\t\t\t\t\t\t<a class=\"elementor-accordion-title\" tabindex=\"0\">Why do my cells die after electroporation?<\/a>\n\t\t\t\t\t<\/div>\n\t\t\t\t\t<div id=\"elementor-tab-content-1373\" class=\"elementor-tab-content elementor-clearfix\" data-tab=\"3\" role=\"region\" aria-labelledby=\"elementor-tab-title-1373\"><p>Some cell death is normal. At the right voltage, about 10\u201320% of cells die after Celetrix electroporation; if almost none die, the voltage may be too low. If there is a lot of cell death, the voltage is likely too high: lower it by 20\u201330 V and test 2\u20133 voltages. Always recover freshly electroporated cells in antibiotic-free medium.<\/p><\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t\t\t<div class=\"elementor-accordion-item\">\n\t\t\t\t\t<div id=\"elementor-tab-title-1374\" class=\"elementor-tab-title\" data-tab=\"4\" role=\"button\" aria-controls=\"elementor-tab-content-1374\" aria-expanded=\"false\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t<span class=\"elementor-accordion-icon elementor-accordion-icon-left\" aria-hidden=\"true\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<span class=\"elementor-accordion-icon-closed\"><svg class=\"e-font-icon-svg e-fas-plus\" viewBox=\"0 0 448 512\" xmlns=\"http:\/\/www.w3.org\/2000\/svg\"><path d=\"M416 208H272V64c0-17.67-14.33-32-32-32h-32c-17.67 0-32 14.33-32 32v144H32c-17.67 0-32 14.33-32 32v32c0 17.67 14.33 32 32 32h144v144c0 17.67 14.33 32 32 32h32c17.67 0 32-14.33 32-32V304h144c17.67 0 32-14.33 32-32v-32c0-17.67-14.33-32-32-32z\"><\/path><\/svg><\/span>\n\t\t\t\t\t\t\t\t<span class=\"elementor-accordion-icon-opened\"><svg class=\"e-font-icon-svg e-fas-minus\" viewBox=\"0 0 448 512\" xmlns=\"http:\/\/www.w3.org\/2000\/svg\"><path d=\"M416 208H32c-17.67 0-32 14.33-32 32v32c0 17.67 14.33 32 32 32h384c17.67 0 32-14.33 32-32v-32c0-17.67-14.33-32-32-32z\"><\/path><\/svg><\/span>\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/span>\n\t\t\t\t\t\t\t\t\t\t\t\t<a class=\"elementor-accordion-title\" tabindex=\"0\">Why does electroporation give better cell viability than transfection reagents?<\/a>\n\t\t\t\t\t<\/div>\n\t\t\t\t\t<div id=\"elementor-tab-content-1374\" class=\"elementor-tab-content elementor-clearfix\" data-tab=\"4\" role=\"region\" aria-labelledby=\"elementor-tab-title-1374\"><p>Electroporation acts at the cell membrane, so its small toxicity stays at the membrane and has little effect inside the cell. Lipid and other chemical transfection reagents affect not only the membrane but also components inside the cell. That is why Celetrix electroporation keeps cell toxicity low and cells healthier than transfection reagents. Learn how the voltage falls on the cell membrane in our <a href=\"https:\/\/www.celetrix.com\/electroporation-mechanism\/\">electroporation mechanism<\/a> page.<\/p><\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t\t\t<div class=\"elementor-accordion-item\">\n\t\t\t\t\t<div id=\"elementor-tab-title-1375\" class=\"elementor-tab-title\" data-tab=\"5\" role=\"button\" aria-controls=\"elementor-tab-content-1375\" aria-expanded=\"false\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t<span class=\"elementor-accordion-icon elementor-accordion-icon-left\" aria-hidden=\"true\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<span class=\"elementor-accordion-icon-closed\"><svg class=\"e-font-icon-svg e-fas-plus\" viewBox=\"0 0 448 512\" xmlns=\"http:\/\/www.w3.org\/2000\/svg\"><path d=\"M416 208H272V64c0-17.67-14.33-32-32-32h-32c-17.67 0-32 14.33-32 32v144H32c-17.67 0-32 14.33-32 32v32c0 17.67 14.33 32 32 32h144v144c0 17.67 14.33 32 32 32h32c17.67 0 32-14.33 32-32V304h144c17.67 0 32-14.33 32-32v-32c0-17.67-14.33-32-32-32z\"><\/path><\/svg><\/span>\n\t\t\t\t\t\t\t\t<span class=\"elementor-accordion-icon-opened\"><svg class=\"e-font-icon-svg e-fas-minus\" viewBox=\"0 0 448 512\" xmlns=\"http:\/\/www.w3.org\/2000\/svg\"><path d=\"M416 208H32c-17.67 0-32 14.33-32 32v32c0 17.67 14.33 32 32 32h384c17.67 0 32-14.33 32-32v-32c0-17.67-14.33-32-32-32z\"><\/path><\/svg><\/span>\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/span>\n\t\t\t\t\t\t\t\t\t\t\t\t<a class=\"elementor-accordion-title\" tabindex=\"0\">How do I choose electroporation parameters?<\/a>\n\t\t\t\t\t<\/div>\n\t\t\t\t\t<div id=\"elementor-tab-content-1375\" class=\"elementor-tab-content elementor-clearfix\" data-tab=\"5\" role=\"region\" aria-labelledby=\"elementor-tab-title-1375\"><p>Start from the <a href=\"\/recommend-parameters\/\">Celetrix recommended parameters<\/a> for your cell type. On Celetrix electroporators, large cells such as cell lines and activated T, B and NK cells usually need 460\u2013600 V in Cell Line mode (20 \u00b5l tube), while small cells such as unstimulated PBMC need a higher voltage in PBMC mode. Keep cell density fixed, then fine-tune the voltage in 20 V steps.<\/p><\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t\t\t<div class=\"elementor-accordion-item\">\n\t\t\t\t\t<div id=\"elementor-tab-title-1376\" class=\"elementor-tab-title\" data-tab=\"6\" role=\"button\" aria-controls=\"elementor-tab-content-1376\" aria-expanded=\"false\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t<span class=\"elementor-accordion-icon elementor-accordion-icon-left\" aria-hidden=\"true\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<span class=\"elementor-accordion-icon-closed\"><svg class=\"e-font-icon-svg e-fas-plus\" viewBox=\"0 0 448 512\" xmlns=\"http:\/\/www.w3.org\/2000\/svg\"><path d=\"M416 208H272V64c0-17.67-14.33-32-32-32h-32c-17.67 0-32 14.33-32 32v144H32c-17.67 0-32 14.33-32 32v32c0 17.67 14.33 32 32 32h144v144c0 17.67 14.33 32 32 32h32c17.67 0 32-14.33 32-32V304h144c17.67 0 32-14.33 32-32v-32c0-17.67-14.33-32-32-32z\"><\/path><\/svg><\/span>\n\t\t\t\t\t\t\t\t<span class=\"elementor-accordion-icon-opened\"><svg class=\"e-font-icon-svg e-fas-minus\" viewBox=\"0 0 448 512\" xmlns=\"http:\/\/www.w3.org\/2000\/svg\"><path d=\"M416 208H32c-17.67 0-32 14.33-32 32v32c0 17.67 14.33 32 32 32h384c17.67 0 32-14.33 32-32v-32c0-17.67-14.33-32-32-32z\"><\/path><\/svg><\/span>\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/span>\n\t\t\t\t\t\t\t\t\t\t\t\t<a class=\"elementor-accordion-title\" tabindex=\"0\">Why does Celetrix give a voltage range instead of a single voltage?<\/a>\n\t\t\t\t\t<\/div>\n\t\t\t\t\t<div id=\"elementor-tab-content-1376\" class=\"elementor-tab-content elementor-clearfix\" data-tab=\"6\" role=\"region\" aria-labelledby=\"elementor-tab-title-1376\"><p>The best voltage depends on your sample. Higher cell density needs a slightly higher voltage. With larger plasmids, cells tolerate high voltage less well, so lower the voltage slightly. That is why Celetrix gives a voltage range instead of one fixed voltage: choose a value within the range to match your cell density and plasmid size.<\/p><\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t\t\t<div class=\"elementor-accordion-item\">\n\t\t\t\t\t<div id=\"elementor-tab-title-1377\" class=\"elementor-tab-title\" data-tab=\"7\" role=\"button\" aria-controls=\"elementor-tab-content-1377\" aria-expanded=\"false\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t<span class=\"elementor-accordion-icon elementor-accordion-icon-left\" aria-hidden=\"true\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<span class=\"elementor-accordion-icon-closed\"><svg class=\"e-font-icon-svg e-fas-plus\" viewBox=\"0 0 448 512\" xmlns=\"http:\/\/www.w3.org\/2000\/svg\"><path d=\"M416 208H272V64c0-17.67-14.33-32-32-32h-32c-17.67 0-32 14.33-32 32v144H32c-17.67 0-32 14.33-32 32v32c0 17.67 14.33 32 32 32h144v144c0 17.67 14.33 32 32 32h32c17.67 0 32-14.33 32-32V304h144c17.67 0 32-14.33 32-32v-32c0-17.67-14.33-32-32-32z\"><\/path><\/svg><\/span>\n\t\t\t\t\t\t\t\t<span class=\"elementor-accordion-icon-opened\"><svg class=\"e-font-icon-svg e-fas-minus\" viewBox=\"0 0 448 512\" xmlns=\"http:\/\/www.w3.org\/2000\/svg\"><path d=\"M416 208H32c-17.67 0-32 14.33-32 32v32c0 17.67 14.33 32 32 32h384c17.67 0 32-14.33 32-32v-32c0-17.67-14.33-32-32-32z\"><\/path><\/svg><\/span>\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/span>\n\t\t\t\t\t\t\t\t\t\t\t\t<a class=\"elementor-accordion-title\" tabindex=\"0\">How many cells do I need for electroporation?<\/a>\n\t\t\t\t\t<\/div>\n\t\t\t\t\t<div id=\"elementor-tab-content-1377\" class=\"elementor-tab-content elementor-clearfix\" data-tab=\"7\" role=\"region\" aria-labelledby=\"elementor-tab-title-1377\"><p>For Celetrix electroporation of cell lines, use 0.5\u20132\u00d710<sup>6<\/sup> cells per 20 \u00b5l tube (typically 1\u00d710<sup>6<\/sup>) or 2.5\u201310\u00d710<sup>6<\/sup> per 100 \u00b5l. Celetrix tubes scale from 20 \u00b5l to 1 ml with the same protocol.<\/p><\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t\t\t<div class=\"elementor-accordion-item\">\n\t\t\t\t\t<div id=\"elementor-tab-title-1378\" class=\"elementor-tab-title\" data-tab=\"8\" role=\"button\" aria-controls=\"elementor-tab-content-1378\" aria-expanded=\"false\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t<span class=\"elementor-accordion-icon elementor-accordion-icon-left\" aria-hidden=\"true\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<span class=\"elementor-accordion-icon-closed\"><svg class=\"e-font-icon-svg e-fas-plus\" viewBox=\"0 0 448 512\" xmlns=\"http:\/\/www.w3.org\/2000\/svg\"><path d=\"M416 208H272V64c0-17.67-14.33-32-32-32h-32c-17.67 0-32 14.33-32 32v144H32c-17.67 0-32 14.33-32 32v32c0 17.67 14.33 32 32 32h144v144c0 17.67 14.33 32 32 32h32c17.67 0 32-14.33 32-32V304h144c17.67 0 32-14.33 32-32v-32c0-17.67-14.33-32-32-32z\"><\/path><\/svg><\/span>\n\t\t\t\t\t\t\t\t<span class=\"elementor-accordion-icon-opened\"><svg class=\"e-font-icon-svg e-fas-minus\" viewBox=\"0 0 448 512\" xmlns=\"http:\/\/www.w3.org\/2000\/svg\"><path d=\"M416 208H32c-17.67 0-32 14.33-32 32v32c0 17.67 14.33 32 32 32h384c17.67 0 32-14.33 32-32v-32c0-17.67-14.33-32-32-32z\"><\/path><\/svg><\/span>\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/span>\n\t\t\t\t\t\t\t\t\t\t\t\t<a class=\"elementor-accordion-title\" tabindex=\"0\">What buffer should I use for electroporation?<\/a>\n\t\t\t\t\t<\/div>\n\t\t\t\t\t<div id=\"elementor-tab-content-1378\" class=\"elementor-tab-content elementor-clearfix\" data-tab=\"8\" role=\"region\" aria-labelledby=\"elementor-tab-title-1378\"><p>Use Celetrix electroporation buffer. It protects cells during the pulse and is matched to Celetrix voltage settings, so results stay consistent from small to large volumes.<\/p><\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t\t\t<div class=\"elementor-accordion-item\">\n\t\t\t\t\t<div id=\"elementor-tab-title-1379\" class=\"elementor-tab-title\" data-tab=\"9\" role=\"button\" aria-controls=\"elementor-tab-content-1379\" aria-expanded=\"false\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t<span class=\"elementor-accordion-icon elementor-accordion-icon-left\" aria-hidden=\"true\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<span class=\"elementor-accordion-icon-closed\"><svg class=\"e-font-icon-svg e-fas-plus\" viewBox=\"0 0 448 512\" xmlns=\"http:\/\/www.w3.org\/2000\/svg\"><path d=\"M416 208H272V64c0-17.67-14.33-32-32-32h-32c-17.67 0-32 14.33-32 32v144H32c-17.67 0-32 14.33-32 32v32c0 17.67 14.33 32 32 32h144v144c0 17.67 14.33 32 32 32h32c17.67 0 32-14.33 32-32V304h144c17.67 0 32-14.33 32-32v-32c0-17.67-14.33-32-32-32z\"><\/path><\/svg><\/span>\n\t\t\t\t\t\t\t\t<span class=\"elementor-accordion-icon-opened\"><svg class=\"e-font-icon-svg e-fas-minus\" viewBox=\"0 0 448 512\" xmlns=\"http:\/\/www.w3.org\/2000\/svg\"><path d=\"M416 208H32c-17.67 0-32 14.33-32 32v32c0 17.67 14.33 32 32 32h384c17.67 0 32-14.33 32-32v-32c0-17.67-14.33-32-32-32z\"><\/path><\/svg><\/span>\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/span>\n\t\t\t\t\t\t\t\t\t\t\t\t<a class=\"elementor-accordion-title\" tabindex=\"0\">What cell types can be electroporated?<\/a>\n\t\t\t\t\t<\/div>\n\t\t\t\t\t<div id=\"elementor-tab-content-1379\" class=\"elementor-tab-content elementor-clearfix\" data-tab=\"9\" role=\"region\" aria-labelledby=\"elementor-tab-title-1379\"><p>Celetrix electroporation works with a wide range of cells: cell lines (e.g., HEK293, CHO, Jurkat, K562), primary cells, stem cells such as iPSCs and MSCs, and PBMC, T, B and NK cells. See the <a href=\"\/recommend-parameters\/\">Celetrix recommended parameters<\/a> for more than 60 cell types.<\/p><\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t\t\t<div class=\"elementor-accordion-item\">\n\t\t\t\t\t<div id=\"elementor-tab-title-13710\" class=\"elementor-tab-title\" data-tab=\"10\" role=\"button\" aria-controls=\"elementor-tab-content-13710\" aria-expanded=\"false\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t<span class=\"elementor-accordion-icon elementor-accordion-icon-left\" aria-hidden=\"true\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<span class=\"elementor-accordion-icon-closed\"><svg class=\"e-font-icon-svg e-fas-plus\" viewBox=\"0 0 448 512\" xmlns=\"http:\/\/www.w3.org\/2000\/svg\"><path d=\"M416 208H272V64c0-17.67-14.33-32-32-32h-32c-17.67 0-32 14.33-32 32v144H32c-17.67 0-32 14.33-32 32v32c0 17.67 14.33 32 32 32h144v144c0 17.67 14.33 32 32 32h32c17.67 0 32-14.33 32-32V304h144c17.67 0 32-14.33 32-32v-32c0-17.67-14.33-32-32-32z\"><\/path><\/svg><\/span>\n\t\t\t\t\t\t\t\t<span class=\"elementor-accordion-icon-opened\"><svg class=\"e-font-icon-svg e-fas-minus\" viewBox=\"0 0 448 512\" xmlns=\"http:\/\/www.w3.org\/2000\/svg\"><path d=\"M416 208H32c-17.67 0-32 14.33-32 32v32c0 17.67 14.33 32 32 32h384c17.67 0 32-14.33 32-32v-32c0-17.67-14.33-32-32-32z\"><\/path><\/svg><\/span>\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/span>\n\t\t\t\t\t\t\t\t\t\t\t\t<a class=\"elementor-accordion-title\" tabindex=\"0\">Do Cas9 RNP and siRNA need a different voltage than plasmid?<\/a>\n\t\t\t\t\t<\/div>\n\t\t\t\t\t<div id=\"elementor-tab-content-13710\" class=\"elementor-tab-content elementor-clearfix\" data-tab=\"10\" role=\"region\" aria-labelledby=\"elementor-tab-title-13710\"><p>Yes. With Celetrix electroporators, Cas9 RNP and siRNA usually need a slightly higher voltage than small plasmids, while large plasmids need a lower voltage.<\/p><\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t\t\t\t\t\t<script type=\"application\/ld+json\">{\"@context\":\"https:\\\/\\\/schema.org\",\"@type\":\"FAQPage\",\"mainEntity\":[{\"@type\":\"Question\",\"name\":\"What is electroporation and how does it work?\",\"acceptedAnswer\":{\"@type\":\"Answer\",\"text\":\"<p>Electroporation uses a brief electric pulse to drive DNA, mRNA, siRNA, proteins or Cas9 RNP across the cell membrane. The voltage falls mostly on the cell membrane, so the inside of the cell is protected. Celetrix electroporators suspend cells in a protective Celetrix buffer to keep viability high.<\\\/p>\"}},{\"@type\":\"Question\",\"name\":\"How can I increase electroporation efficiency?\",\"acceptedAnswer\":{\"@type\":\"Answer\",\"text\":\"<p>With Celetrix electroporation, start from the recommended voltage for your cell type and test 3 voltages, 20 V apart, with a GFP plasmid. Use healthy, single cells and electroporate each sample right after loading the tube.<\\\/p>\"}},{\"@type\":\"Question\",\"name\":\"Why do my cells die after electroporation?\",\"acceptedAnswer\":{\"@type\":\"Answer\",\"text\":\"<p>Some cell death is normal. At the right voltage, about 10\\u201320% of cells die after Celetrix electroporation; if almost none die, the voltage may be too low. If there is a lot of cell death, the voltage is likely too high: lower it by 20\\u201330 V and test 2\\u20133 voltages. Always recover freshly electroporated cells in antibiotic-free medium.<\\\/p>\"}},{\"@type\":\"Question\",\"name\":\"Why does electroporation give better cell viability than transfection reagents?\",\"acceptedAnswer\":{\"@type\":\"Answer\",\"text\":\"<p>Electroporation acts at the cell membrane, so its small toxicity stays at the membrane and has little effect inside the cell. Lipid and other chemical transfection reagents affect not only the membrane but also components inside the cell. That is why Celetrix electroporation keeps cell toxicity low and cells healthier than transfection reagents. Learn how the voltage falls on the cell membrane in our <a href=\\\"https:\\\/\\\/www.celetrix.com\\\/electroporation-mechanism\\\/\\\">electroporation mechanism<\\\/a> page.<\\\/p>\"}},{\"@type\":\"Question\",\"name\":\"How do I choose electroporation parameters?\",\"acceptedAnswer\":{\"@type\":\"Answer\",\"text\":\"<p>Start from the <a href=\\\"\\\/recommend-parameters\\\/\\\">Celetrix recommended parameters<\\\/a> for your cell type. On Celetrix electroporators, large cells such as cell lines and activated T, B and NK cells usually need 460\\u2013600 V in Cell Line mode (20 \\u00b5l tube), while small cells such as unstimulated PBMC need a higher voltage in PBMC mode. Keep cell density fixed, then fine-tune the voltage in 20 V steps.<\\\/p>\"}},{\"@type\":\"Question\",\"name\":\"Why does Celetrix give a voltage range instead of a single voltage?\",\"acceptedAnswer\":{\"@type\":\"Answer\",\"text\":\"<p>The best voltage depends on your sample. Higher cell density needs a slightly higher voltage. With larger plasmids, cells tolerate high voltage less well, so lower the voltage slightly. That is why Celetrix gives a voltage range instead of one fixed voltage: choose a value within the range to match your cell density and plasmid size.<\\\/p>\"}},{\"@type\":\"Question\",\"name\":\"How many cells do I need for electroporation?\",\"acceptedAnswer\":{\"@type\":\"Answer\",\"text\":\"<p>For Celetrix electroporation of cell lines, use 0.5\\u20132\\u00d710<sup>6<\\\/sup> cells per 20 \\u00b5l tube (typically 1\\u00d710<sup>6<\\\/sup>) or 2.5\\u201310\\u00d710<sup>6<\\\/sup> per 100 \\u00b5l. Celetrix tubes scale from 20 \\u00b5l to 1 ml with the same protocol.<\\\/p>\"}},{\"@type\":\"Question\",\"name\":\"What buffer should I use for electroporation?\",\"acceptedAnswer\":{\"@type\":\"Answer\",\"text\":\"<p>Use Celetrix electroporation buffer. It protects cells during the pulse and is matched to Celetrix voltage settings, so results stay consistent from small to large volumes.<\\\/p>\"}},{\"@type\":\"Question\",\"name\":\"What cell types can be electroporated?\",\"acceptedAnswer\":{\"@type\":\"Answer\",\"text\":\"<p>Celetrix electroporation works with a wide range of cells: cell lines (e.g., HEK293, CHO, Jurkat, K562), primary cells, stem cells such as iPSCs and MSCs, and PBMC, T, B and NK cells. See the <a href=\\\"\\\/recommend-parameters\\\/\\\">Celetrix recommended parameters<\\\/a> for more than 60 cell types.<\\\/p>\"}},{\"@type\":\"Question\",\"name\":\"Do Cas9 RNP and siRNA need a different voltage than plasmid?\",\"acceptedAnswer\":{\"@type\":\"Answer\",\"text\":\"<p>Yes. With Celetrix electroporators, Cas9 RNP and siRNA usually need a slightly higher voltage than small plasmids, while large plasmids need a lower voltage.<\\\/p>\"}}]}<\/script>\n\t\t\t\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-e20d971 elementor-align-center elementor-widget elementor-widget-button\" data-id=\"e20d971\" data-element_type=\"widget\" data-widget_type=\"button.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<div class=\"elementor-button-wrapper\">\n\t\t\t\t\t<a class=\"elementor-button elementor-button-link elementor-size-sm\" href=\"https:\/\/www.celetrix.com\/ordering\/\">\n\t\t\t\t\t\t<span class=\"elementor-button-content-wrapper\">\n\t\t\t\t\t\t\t\t\t<span class=\"elementor-button-text\">Request a Quote<\/span>\n\t\t\t\t\t<\/span>\n\t\t\t\t\t<\/a>\n\t\t\t\t<\/div>\n\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t<div class=\"elementor-column elementor-col-50 elementor-top-column elementor-element elementor-element-484c94a\" data-id=\"484c94a\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-ebc67db elementor-nav-menu__align-start elementor-nav-menu--dropdown-none elementor-widget elementor-widget-nav-menu\" data-id=\"ebc67db\" data-element_type=\"widget\" data-settings=\"{&quot;layout&quot;:&quot;vertical&quot;,&quot;submenu_icon&quot;:{&quot;value&quot;:&quot;&lt;svg aria-hidden=\\&quot;true\\&quot; 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Start from our recommended parameters, run a quick GFP plasmid test, and fine-tune the voltage for your cell density, temperature and payload. Quick Optimization Workflow Start from the recommended voltage for your cell type. For unlisted cells, use a cell type [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"elementor_header_footer","meta":{"om_disable_all_campaigns":false,"_uf_show_specific_survey":0,"_uf_disable_surveys":false,"footnotes":""},"class_list":["post-53","page","type-page","status-publish","hentry"],"aioseo_notices":[],"_links":{"self":[{"href":"https:\/\/www.celetrix.com\/zh\/wp-json\/wp\/v2\/pages\/53","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.celetrix.com\/zh\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/www.celetrix.com\/zh\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/www.celetrix.com\/zh\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.celetrix.com\/zh\/wp-json\/wp\/v2\/comments?post=53"}],"version-history":[{"count":15,"href":"https:\/\/www.celetrix.com\/zh\/wp-json\/wp\/v2\/pages\/53\/revisions"}],"predecessor-version":[{"id":2580,"href":"https:\/\/www.celetrix.com\/zh\/wp-json\/wp\/v2\/pages\/53\/revisions\/2580"}],"wp:attachment":[{"href":"https:\/\/www.celetrix.com\/zh\/wp-json\/wp\/v2\/media?parent=53"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}