{"id":51,"date":"2021-10-28T08:17:08","date_gmt":"2021-10-28T08:17:08","guid":{"rendered":"https:\/\/celetrix.com\/celetrix\/?page_id=51"},"modified":"2026-10-26T08:30:37","modified_gmt":"2026-10-26T08:30:37","slug":"recommend-parameters","status":"publish","type":"page","link":"https:\/\/www.celetrix.com\/zh\/recommend-parameters\/","title":{"rendered":"Recommended Parameters"},"content":{"rendered":"<div data-elementor-type=\"wp-page\" data-elementor-id=\"51\" class=\"elementor elementor-51\" data-elementor-post-type=\"page\">\n\t\t\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-3098acb2 elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"3098acb2\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-50 elementor-top-column elementor-element elementor-element-27dcf09b\" data-id=\"27dcf09b\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-78846aeb elementor-widget elementor-widget-bdt-breadcrumbs\" data-id=\"78846aeb\" data-element_type=\"widget\" data-widget_type=\"bdt-breadcrumbs.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<div class='bdt-breadcrumbs-wrapper'><ul class=\"bdt-breadcrumb breadcrumb-align-left\"><li><a href=\"https:\/\/www.celetrix.com\/zh\/\"><span class=\"bdt-breadcrumbs-home-icon\"> <i aria-hidden=\"true\" class=\"fas fa-home\"><\/i> <\/span>Electroporation by Celetrix<\/a><\/li>\n<li><span> \u9875\u9762<\/span><\/li>\n<\/ul><\/div>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-99b7189 elementor-widget elementor-widget-heading\" data-id=\"99b7189\" data-element_type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h1 class=\"elementor-heading-title elementor-size-default\">Electroporation Parameters by Cell Type<\/h1>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-0e8e3e9 elementor-widget elementor-widget-text-editor\" data-id=\"0e8e3e9\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p>Recommended Celetrix electroporation voltages for primary cells, stem cells and cell lines. Find your cell type below; linked cell types have a full protocol page with data. For cells not listed, start from a cell type of similar size and fine-tune with our <a href=\"https:\/\/www.celetrix.com\/zh\/optimization-strategy\/\">optimization guide<\/a>.<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-a6d5a85 elementor-widget elementor-widget-heading\" data-id=\"a6d5a85\" data-element_type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h2 class=\"elementor-heading-title elementor-size-default\">Recommended Parameters<\/h2>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-f99ee30 elementor-widget elementor-widget-bdt-table\" data-id=\"f99ee30\" data-element_type=\"widget\" data-widget_type=\"bdt-table.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t          <div class=\"bdt-table bdt-overflow-auto  bdt-data-table\" id=\"bdt-table-f99ee30\" data-settings=\"{&quot;paging&quot;:false,&quot;info&quot;:false,&quot;searching&quot;:true,&quot;ordering&quot;:false}\">\n              \n              \n              <table>\n    <thead>\n        <tr>\n            <th>\n                Cell&nbsp;Type&nbsp;\n            <\/th>\n            <th>\n                Mode\n            <\/th>\n            <th>\n                20 \u00b5l, 100 \u00b5l\n            <\/th>\n            <th>\n                200 \u00b5l, 1 ml\n            <\/th>\n        <\/tr>\n    <\/thead>\n   <tbody>\n        <tr>\n            <td>\n               <a href=\"\/zh\/pbmc\/\">Human&nbsp;PBMC<\/a>\n            <\/td>\n            <td>\n                PBMC\n            <\/td>\n            <td>\n                1200&nbsp;-1400\n            <\/td>\n            <td>\n                NA\n            <\/td>\n        <\/tr>\n        <tr>\n            <td>\n                Human&nbsp;T&nbsp;cells,&nbsp;unstimulated\n            <\/td>\n            <td>\n                PBMC\n            <\/td>\n            <td>\n                1300&nbsp;-1450\n            <\/td>\n            <td>\n                NA\n            <\/td>\n        <\/tr>\n        <tr>\n            <td>\n                Human&nbsp;B&nbsp;cells,&nbsp;unstimulated\n            <\/td>\n            <td>\n                PBMC\n            <\/td>\n            <td>\n                1300&nbsp;-1450\n            <\/td>\n            <td>\n                NA\n            <\/td>\n        <\/tr>\n        <tr>\n            <td>\n                Human&nbsp;NK&nbsp;cells,&nbsp;unstimulated\n            <\/td>\n            <td>\n                PBMC\n            <\/td>\n            <td>\n                1300&nbsp;-1450\n            <\/td>\n            <td>\n                NA\n            <\/td>\n        <\/tr>\n        <tr>\n            <td>\n                 <a href=\"\/zh\/human-neutrophil\/\">Human&nbsp;Neutrophil\n            <\/td>\n            <td>\n                PBMC\n            <\/td>\n            <td>\n               1300&nbsp;-1450\n            <\/td>\n            <td>\n                NA\n            <\/td>\n        <\/tr>\n        <tr>\n            <td>\n                Mouse&nbsp;lymph&nbsp;node&nbsp;primary&nbsp;T&nbsp;cell\n            <\/td>\n            <td>\n                PBMC\n            <\/td>\n            <td>\n                1300&nbsp;-1450\n            <\/td>\n            <td>\n                NA\n            <\/td>\n        <\/tr>\n        <tr>\n            <td>\n                <a href=\"\/zh\/mouse-primary-spleen-cell\/\">Mouse&nbsp;primary&nbsp;spleen&nbsp;cells\n            <\/td>\n            <td>\n                PBMC\n            <\/td>\n            <td>\n                1300&nbsp;-1450\n            <\/td>\n            <td>\n                NA\n            <\/td>\n        <\/tr>\n        <tr>\n            <td>\n                  <a href=\"\/zh\/mouse-monocyte\/\">Mouse&nbsp;bone&nbsp;marrow&nbsp;monocyte\n            <\/td>\n            <td>\n                PBMC\n            <\/td>\n            <td>\n                1300&nbsp;-1450\n            <\/td>\n            <td>\n                NA\n            <\/td>\n        <\/tr>\n \n<tr>\n            <td>\n                <a href=\"\/zh\/human-cultured-t-cells\/\">Human&nbsp;Cultured&nbsp;T&nbsp;cells\n            <\/td>\n            <td>\n                Cell&nbsp;Line\n            <\/td>\n            <td>\n                480-540\n            <\/td>\n            <td>\n                1150-1300\n            <\/td>\n        <\/tr>\n        <tr>\n            <td>\n               <a href=\"\/zh\/cultured-nk-cells\/\">Human&nbsp;Cultured&nbsp;NK&nbsp;cells\n            <\/td>\n            <td>\n                Cell&nbsp;Line\n            <\/td>\n            <td>\n                480-540\n            <\/td>\n            <td>\n                1200-1300\n            <\/td>\n        <\/tr>\n        <tr>\n            <td>\n               <a href=\"\/zh\/human-ipsc\/\"> Human&nbsp;iPSC\n            <\/td>\n            <td>\n                Cell&nbsp;Line\n            <\/td>\n            <td>\n                500-540\n            <\/td>\n            <td>\n                1200-1300\n            <\/td>\n        <\/tr>\n        <tr>\n            <td>\n                Human&nbsp;MM1.S\n            <\/td>\n            <td>\n                Cell&nbsp;Line\n            <\/td>\n            <td>\n                520-560\n            <\/td>\n            <td>\n                1250-1350\n            <\/td>\n        <\/tr>\n        <tr>\n            <td>\n                Human&nbsp;adipose&nbsp;MSC\n            <\/td>\n            <td>\n                Cell&nbsp;Line\n            <\/td>\n            <td>\n                500-540\n            <\/td>\n            <td>\n                1200-1300\n            <\/td>\n        <\/tr>\n        <tr>\n            <td>\n                Human&nbsp;bone&nbsp;marrow&nbsp;MSC\n            <\/td>\n            <td>\n                Cell&nbsp;Line\n            <\/td>\n            <td>\n                500-540\n            <\/td>\n            <td>\n                1200-1300\n            <\/td>\n        <\/tr>\n\n        <tr>\n            <td>\n                Human&nbsp;cord&nbsp;blood&nbsp;MSC\n            <\/td>\n            <td>\n                Cell&nbsp;Line\n            <\/td>\n            <td>\n                500-540\n            <\/td>\n            <td>\n                1200-1300\n            <\/td>\n        <\/tr>\n        <tr>\n            <td>\n                Human&nbsp;ESC&nbsp;derived&nbsp;neural&nbsp;stem&nbsp;cell\n            <\/td>\n            <td>\n                Cell&nbsp;Line\n            <\/td>\n            <td>\n                520-560\n            <\/td>\n            <td>\n                1250-1350\n            <\/td>\n        <\/tr>\n        <tr>\n            <td>\n                Human&nbsp;primary&nbsp;hepatocyte\n            <\/td>\n            <td>\n                Cell&nbsp;Line\n            <\/td>\n            <td>\n                480-520\n            <\/td>\n            <td>\n                1150-1250\n            <\/td>\n        <\/tr>\n        <tr>\n            <td>\n                <a href=\"\/zh\/human-primary-dendritic-cells\/\">Human&nbsp;primary&nbsp;Dendritic&nbsp;cells&nbsp;\n            <\/td>\n            <td>\n                Cell&nbsp;Line\n            <\/td>\n            <td>\n                480-520\n            <\/td>\n            <td>\n                1150-1200\n            <\/td>\n        <\/tr>\n        <tr>\n            <td>\n                Human&nbsp;neural&nbsp;progenitor&nbsp;ReNCell&nbsp;VM\n            <\/td>\n            <td>\n                Cell&nbsp;Line\n            <\/td>\n            <td>\n                520-560\n            <\/td>\n            <td>\n                1250-1350\n            <\/td>\n        <\/tr>\n        <tr>\n            <td>\n                Mouse&nbsp;iPSC\n            <\/td>\n            <td>\n                Cell&nbsp;Line\n            <\/td>\n            <td>\n                480-520\n            <\/td>\n            <td>\n                1150-1250\n            <\/td>\n        <\/tr>\n        <tr>\n            <td>\n                Mouse&nbsp;ESC\n            <\/td>\n            <td>\n                Cell&nbsp;Line\n            <\/td>\n            <td>\n                480-520\n            <\/td>\n            <td>\n                1150-1250\n            <\/td>\n        <\/tr>\n        <tr>\n            <td>\n                 <a href=\"\/zh\/mouse-lymph-node-cultured-t-cell\/\">Mouse&nbsp;lymph&nbsp;node&nbsp;Cultured&nbsp;T&nbsp;cell\n            <\/td>\n            <td>\n                Cell&nbsp;Line\n            <\/td>\n            <td>\n                500-540\n            <\/td>\n            <td>\n                1200-1300\n            <\/td>\n        <\/tr>\n        <tr>\n            <td>\n                 <a href=\"\/zh\/mouse-peritoneal-macrophage\/\"> Mouse&nbsp;peritoneal&nbsp;Macrophage\n            <\/td>\n            <td>\n                Cell&nbsp;Line\n            <\/td>\n            <td>\n                540-580\n            <\/td>\n            <td>\n                1350-1400\n            <\/td>\n        <\/tr>\n        <tr>\n            <td>\n                 <a href=\"\/zh\/primary-mouse-cortical-neurons\/\">Primary&nbsp;mouse&nbsp;cortical&nbsp;neurons\n            <\/td>\n            <td>\n                Cell&nbsp;Line\n            <\/td>\n            <td>\n                520-560\n            <\/td>\n            <td>\n                1350-1400\n            <\/td>\n        <\/tr>\n        <tr>\n            <td>\n                Rat&nbsp;primary&nbsp;cortex&nbsp;neural&nbsp;progenitor&nbsp;cell\n            <\/td>\n            <td>\n                Cell&nbsp;Line\n            <\/td>\n            <td>\n                520-560\n            <\/td>\n            <td>\n                1250-1350\n            <\/td>\n        <\/tr>\n        <tr>\n            <td>\n                293\n            <\/td>\n            <td>\n                Cell&nbsp;Line\n            <\/td>\n            <td>\n                440-480\n            <\/td>\n            <td>\n                1050-1150\n            <\/td>\n        <\/tr>\n\n        <tr>\n            <td>\n                   <a href=\"\/zh\/293t\/\">293T\n            <\/td>\n            <td>\n                Cell&nbsp;Line\n            <\/td>\n            <td>\n                460-500\n            <\/td>\n            <td>\n                1050-1150\n            <\/td>\n        <\/tr>\n   <tr>\n            <td>\n                 <a href=\"\/zh\/a20\/\">A20\n            <\/td>\n            <td>\n                Cell&nbsp;Line\n            <\/td>\n            <td>\n                540-560\n            <\/td>\n            <td>\n                1250-1350\n            <\/td>\n        <\/tr>\n        <tr>\n            <td>\n                <a href=\"\/zh\/a549\/\">A549\n            <\/td>\n            <td>\n                Cell&nbsp;Line\n            <\/td>\n            <td>\n                540-560\n            <\/td>\n            <td>\n                1250-1350\n            <\/td>\n        <\/tr>\n        <tr>\n            <td>\n                  <a href=\"\/zh\/adscs\/\">ADSCs\n            <\/td>\n            <td>\n                Cell&nbsp;Line\n            <\/td>\n            <td>\n                500-520\n            <\/td>\n            <td>\n                1200-1300\n            <\/td>\n        <\/tr>\n        <tr>\n            <td>\n                  <a href=\"\/zh\/arpe-19\/\">ARPE-19\n            <\/td>\n            <td>\n                Cell&nbsp;Line\n            <\/td>\n            <td>\n                520-540\n            <\/td>\n            <td>\n                1250-1350\n            <\/td>\n        <\/tr>\n        <tr>\n            <td>\n                 <a href=\"\/zh\/baf3\/\">BaF3\n            <\/td>\n            <td>\n                Cell&nbsp;Line\n            <\/td>\n            <td>\n                520-540\n            <\/td>\n            <td>\n                1250-1350\n            <\/td>\n        <\/tr>\n        <tr>\n            <td>\n                 <a href=\"\/zh\/bj\/\"> BJ\n            <\/td>\n            <td>\n                Cell&nbsp;Line\n            <\/td>\n            <td>\n                500-520\n            <\/td>\n            <td>\n                1250-1400\n            <\/td>\n        <\/tr>\n  <tr>\n            <td>\n                 <a href=\"\/zh\/cal51\/\">CAL51\n            <\/td>\n            <td>\n                Cell&nbsp;Line\n            <\/td>\n            <td>\n                500-520\n            <\/td>\n            <td>\n                1250-1350\n            <\/td>\n        <\/tr>\n        <tr>\n            <td>\n               <a href=\"\/zh\/cho-k1\/\"> CHO-K1\n            <\/td>\n            <td>\n                Cell&nbsp;Line\n            <\/td>\n            <td>\n                560-600\n            <\/td>\n            <td>\n                1250-1400\n            <\/td>\n        <\/tr>\n        <tr>\n            <td>\n                CHO-S\n            <\/td>\n            <td>\n                Cell&nbsp;Line\n            <\/td>\n            <td>\n                520-580\n            <\/td>\n            <td>\n                1250-1400\n            <\/td>\n        <\/tr>\n        <tr>\n            <td>\n                DEL\n            <\/td>\n            <td>\n                Cell&nbsp;Line\n            <\/td>\n            <td>\n                500-540\n            <\/td>\n            <td>\n                1200-1300\n            <\/td>\n        <\/tr>\n  <tr>\n            <td>\n               <a href=\"\/zh\/dld1\/\"> DLD1\n            <\/td>\n            <td>\n                Cell&nbsp;Line\n            <\/td>\n            <td>\n                500-520\n            <\/td>\n            <td>\n                1200-1300\n            <\/td>\n        <\/tr>\n<tr>\n            <td>\n               <a href=\"\/zh\/emt6\/\"> EMT6\n            <\/td>\n            <td>\n                Cell&nbsp;Line\n            <\/td>\n            <td>\n                500-520\n            <\/td>\n            <td>\n                1200-1300\n            <\/td>\n        <\/tr>\n<tr>\n            <td>\n               <a href=\"\/zh\/hct116\/\"> HCT116\n            <\/td>\n            <td>\n                Cell&nbsp;Line\n            <\/td>\n            <td>\n                540-560\n            <\/td>\n            <td>\n                1200-1300\n            <\/td>\n        <\/tr>\n        <tr>\n            <td>\n                <a href=\"\/zh\/hela\/\">Hela\n            <\/td>\n            <td>\n                Cell&nbsp;Line\n            <\/td>\n            <td>\n                480-520\n            <\/td>\n            <td>\n                1250-1350\n            <\/td>\n        <\/tr>\n<tr>\n            <td>\n               <a href=\"\/zh\/hepa1-6\/\"> Hepa1-6\n            <\/td>\n            <td>\n                Cell&nbsp;Line\n            <\/td>\n            <td>\n                500-520\n            <\/td>\n            <td>\n                1200-1300\n            <\/td>\n        <\/tr>\n        <tr>\n            <td>\n               <a href=\"\/zh\/hep3b\/\"> Hep3b\n            <\/td>\n            <td>\n                Cell&nbsp;Line\n            <\/td>\n            <td>\n                500-540\n            <\/td>\n            <td>\n                1100-1200\n            <\/td>\n        <\/tr>\n        <tr>\n            <td>\n               <a href=\"\/zh\/hsf\/\"> HSF\n            <\/td>\n            <td>\n                Cell&nbsp;Line\n            <\/td>\n            <td>\n                520-540\n            <\/td>\n            <td>\n                1250-1350\n            <\/td>\n        <\/tr>\n        <tr>\n            <td>\n               <a href=\"\/zh\/huc-maaa13-p3\/\"> HUC-Maaa13-P3\n            <\/td>\n            <td>\n                Cell&nbsp;Line\n            <\/td>\n            <td>\n                520-540\n            <\/td>\n            <td>\n                1250-1350\n            <\/td>\n        <\/tr>\n        <tr>\n            <td>\n                HUVEC\n            <\/td>\n            <td>\n                Cell&nbsp;Line\n            <\/td>\n            <td>\n                520-560\n            <\/td>\n            <td>\n                1250-1350\n            <\/td>\n        <\/tr>\n        <tr>\n            <td>\n                H1975\n            <\/td>\n            <td>\n                Cell&nbsp;Line\n            <\/td>\n            <td>\n                520-560\n            <\/td>\n            <td>\n                1250-1350\n            <\/td>\n        <\/tr>\n<tr>\n            <td>\n               <a href=\"\/zh\/h22\/\"> H22\n            <\/td>\n            <td>\n                Cell&nbsp;Line\n            <\/td>\n            <td>\n                500-520\n            <\/td>\n            <td>\n                1200-1300\n            <\/td>\n        <\/tr>\n        <tr>\n            <td>\n               <a href=\"\/zh\/h226\/\">  H226\n            <\/td>\n            <td>\n                Cell&nbsp;Line\n            <\/td>\n            <td>\n                520-540\n            <\/td>\n            <td>\n                1250-1350\n            <\/td>\n        <\/tr>\n        <tr>\n            <td>\n                <a href=\"\/zh\/h9c2\/\">H9C2\n            <\/td>\n            <td>\n                Cell&nbsp;Line\n            <\/td>\n            <td>\n                500-540\n            <\/td>\n            <td>\n                1350-1400\n            <\/td>\n        <\/tr>\n        <tr>\n            <td>\n                HL-60&nbsp;*\n            <\/td>\n            <td>\n                Cell&nbsp;Line\n            <\/td>\n            <td>\n                520-560\n            <\/td>\n            <td>\n                1250-1350\n            <\/td>\n        <\/tr>\n        <tr>\n            <td>\n                <a href=\"\/zh\/imc\/\">  iMC\n            <\/td>\n            <td>\n                Cell&nbsp;Line\n            <\/td>\n            <td>\n                500-540\n            <\/td>\n            <td>\n                1200-1300\n            <\/td>\n        <\/tr>\n        <tr>\n            <td>\n                 <a href=\"\/zh\/jurkat\/\"> Jurkat\n            <\/td>\n            <td>\n                Cell&nbsp;Line\n            <\/td>\n            <td>\n                500-540\n            <\/td>\n            <td>\n                1200-1350\n            <\/td>\n        <\/tr>\n        <tr>\n            <td>\n                Karpas299\n            <\/td>\n            <td>\n                Cell&nbsp;Line\n            <\/td>\n            <td>\n                520-560\n            <\/td>\n            <td>\n                1250-1350\n            <\/td>\n        <\/tr>\n        <tr>\n            <td>\n                 <a href=\"\/zh\/k562\/\">K562\n            <\/td>\n            <td>\n                Cell&nbsp;Line\n            <\/td>\n            <td>\n                480-520\n            <\/td>\n            <td>\n                1200-1300\n            <\/td>\n        <\/tr>\n<tr>\n            <td>\n               <a href=\"\/zh\/mc38\/\"> MC38\n            <\/td>\n            <td>\n                Cell&nbsp;Line\n            <\/td>\n            <td>\n                500-520\n            <\/td>\n            <td>\n                1200-1300\n            <\/td>\n        <\/tr>\n<tr>\n            <td>\n               <a href=\"\/zh\/mcf-7\/\"> MCF-7\n            <\/td>\n            <td>\n                Cell&nbsp;Line\n            <\/td>\n            <td>\n                500-520\n            <\/td>\n            <td>\n                1200-1300\n            <\/td>\n        <\/tr>\n        <tr>\n            <td>\n                <a href=\"\/zh\/mda-mb-231\/\"> MDA-MB-231\n            <\/td>\n            <td>\n                Cell&nbsp;Line\n            <\/td>\n            <td>\n                500-520\n            <\/td>\n            <td>\n                1220-1300\n            <\/td>\n        <\/tr>\n        <tr>\n            <td>\n               <a href=\"\/zh\/molm-13\/\"> Molm-13&nbsp;*\n            <\/td>\n            <td>\n                Cell&nbsp;Line\n            <\/td>\n            <td>\n                500-540\n            <\/td>\n            <td>\n                1250-1350\n            <\/td>\n        <\/tr>\n        <tr>\n            <td>\n                <a href=\"\/zh\/nalm-6\/\">Nalm-6\n            <\/td>\n            <td>\n                Cell&nbsp;Line\n            <\/td>\n            <td>\n                520-540\n            <\/td>\n            <td>\n                1250-1350\n            <\/td>\n        <\/tr>\n        <tr>\n            <td>\n               <a href=\"\/zh\/nk92\/\"> NK-92\n            <\/td>\n            <td>\n                Cell&nbsp;Line\n            <\/td>\n            <td>\n                440-460\n            <\/td>\n            <td>\n                1150-1200\n            <\/td>\n        <\/tr>\n        <tr>\n            <td>\n                <a href=\"\/zh\/raji\/\"> Raji\n            <\/td>\n            <td>\n                Cell&nbsp;Line\n            <\/td>\n            <td>\n                520-540\n            <\/td>\n            <td>\n                1250-1350\n            <\/td>\n        <\/tr>\n        <tr>\n            <td>\n                <a href=\"\/zh\/raw264-7\/\">RAW264.7\n            <\/td>\n            <td>\n                Cell&nbsp;Line\n            <\/td>\n            <td>\n                520-540\n            <\/td>\n            <td>\n                1250-1350\n            <\/td>\n        <\/tr>\n        <tr>\n            <td>\n                SF9&nbsp;insect&nbsp;cell\n            <\/td>\n            <td>\n                Cell&nbsp;Line\n            <\/td>\n            <td>\n                480-520\n            <\/td>\n            <td>\n                1200-1300\n            <\/td>\n        <\/tr>\n        <tr>\n            <td>\n                <a href=\"\/zh\/sk-hep-1\/\">SK-HEP-1\n            <\/td>\n            <td>\n                Cell&nbsp;Line\n            <\/td>\n            <td>\n                480-520\n            <\/td>\n            <td>\n                1220-1300\n            <\/td>\n        <\/tr>\n        <tr>\n            <td>\n                SP2\/0\n            <\/td>\n            <td>\n                Cell&nbsp;Line\n            <\/td>\n            <td>\n                480-500\n            <\/td>\n            <td>\n                1150-1200\n            <\/td>\n        <\/tr>\n        <tr>\n            <td>\n                SUP-M2&nbsp;*\n            <\/td>\n            <td>\n                Cell&nbsp;Line\n            <\/td>\n            <td>\n                520-560\n            <\/td>\n            <td>\n                1250-1350\n            <\/td>\n        <\/tr>\n        <tr>\n            <td>\n                <a href=\"\/zh\/swiss-3t3\/\">Swiss-3T3\n            <\/td>\n            <td>\n                Cell&nbsp;Line\n            <\/td>\n            <td>\n                520-540\n            <\/td>\n            <td>\n                1250-1350\n            <\/td>\n        <\/tr>\n        <tr>\n            <td>\n                p3X63Ag8.653\n            <\/td>\n            <td>\n                Cell&nbsp;Line\n            <\/td>\n            <td>\n                500-540\n            <\/td>\n            <td>\n                1220-1300\n            <\/td>\n        <\/tr>\n        <tr>\n            <td>\n               <a href=\"\/zh\/thp-1\/\"> THP-1&nbsp;*\n            <\/td>\n            <td>\n                Cell&nbsp;Line\n            <\/td>\n            <td>\n                500-540\n            <\/td>\n            <td>\n                1250-1300\n            <\/td>\n        <\/tr>\n        <tr>\n            <td>\n                <a href=\"\/zh\/u251\/\"> U251\n            <\/td>\n            <td>\n                Cell&nbsp;Line\n            <\/td>\n            <td>\n                480-520\n            <\/td>\n            <td>\n                1250-1400\n            <\/td>\n        <\/tr>\n        <tr>\n            <td>\n                <a href=\"\/zh\/u937\/\">U937&nbsp;*\n            <\/td>\n            <td>\n                Cell&nbsp;Line\n            <\/td>\n            <td>\n                500-540\n            <\/td>\n            <td>\n                1250-1300\n            <\/td>\n        <\/tr>\n    <\/tbody>\n<\/table>\n          <\/div>\n            \t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-f7a2695 elementor-widget elementor-widget-text-editor\" data-id=\"f7a2695\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p><strong>Mode:<\/strong> Cell Line mode is for cell lines, primary cells and stimulated T, B and NK cells. PBMC mode is for PBMC and unstimulated T, B and NK cells, at room temperature.<\/p>\n<p>*Many monocyte or macrophage related cell lines such as THP-1, U937, HL-60 and Molm13 will die out within two to three days after plasmid electroporation. These cells have no problem with mRNA, siRNA and Cas9 RNP electroporation. The efficiency would be nearly 100% and cell survival is not affected. Celetrix has developed the THP-1 Helper Kit for plasmid electroporation of monocytic cell lines. A significant portion of THP-1 and U937 cells can survive and grow with the helper kit, enabling easy generation of stable cell lines. Our THP-1 Helper Kit does not help cell lines such as Molm13 and SUP-M2 with plasmid electroporation and we are actively researching the solutions.<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-a6a9e26 elementor-widget elementor-widget-heading\" data-id=\"a6a9e26\" data-element_type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h2 class=\"elementor-heading-title elementor-size-default\">Voltage Conversion Between Tube Sizes<\/h2>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-316b17b0 elementor-widget elementor-widget-text-editor\" data-id=\"316b17b0\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p>Different tube sizes need different voltages because of the electrode distance. The pulse time stays the same. Example based on 500 V in a 20 \u00b5l tube:<\/p>\n<p>20 \u00b5l: 500 V<br>100 \u00b5l: 510 V (20 \u00b5l voltage + 10 V)<br>120 \u00b5l: 700 V (20 \u00b5l voltage \u00d7 1.4)<br>200 \u00b5l: 1200 V (20 \u00b5l voltage \u00d7 2.4)<br>600 \u00b5l: 800 V (20 \u00b5l voltage \u00d7 1.6)<br>1 ml: 1250 V (20 \u00b5l voltage \u00d7 2.5)<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t<div class=\"elementor-column elementor-col-50 elementor-top-column elementor-element elementor-element-7224628c\" data-id=\"7224628c\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-4bb03275 elementor-nav-menu__align-start elementor-nav-menu--dropdown-none elementor-widget elementor-widget-nav-menu\" data-id=\"4bb03275\" data-element_type=\"widget\" data-settings=\"{&quot;layout&quot;:&quot;vertical&quot;,&quot;submenu_icon&quot;:{&quot;value&quot;:&quot;&lt;svg aria-hidden=\\&quot;true\\&quot; class=\\&quot;e-font-icon-svg e-fas-caret-down\\&quot; viewBox=\\&quot;0 0 320 512\\&quot; xmlns=\\&quot;http:\\\/\\\/www.w3.org\\\/2000\\\/svg\\&quot;&gt;&lt;path d=\\&quot;M31.3 192h257.3c17.8 0 26.7 21.5 14.1 34.1L174.1 354.8c-7.8 7.8-20.5 7.8-28.3 0L17.2 226.1C4.6 213.5 13.5 192 31.3 192z\\&quot;&gt;&lt;\\\/path&gt;&lt;\\\/svg&gt;&quot;,&quot;library&quot;:&quot;fa-solid&quot;}}\" data-widget_type=\"nav-menu.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t<nav aria-label=\"Menu\" class=\"elementor-nav-menu--main elementor-nav-menu__container elementor-nav-menu--layout-vertical e--pointer-background e--animation-fade\">\n\t\t\t\t<ul id=\"menu-1-4bb03275\" class=\"elementor-nav-menu sm-vertical\"><li class=\"menu-item menu-item-type-post_type menu-item-object-page menu-item-105\"><a href=\"https:\/\/www.celetrix.com\/zh\/manuals-videos\/\" class=\"elementor-item\">Manuals &#038; Videos<\/a><\/li>\n<li class=\"menu-item menu-item-type-post_type menu-item-object-page menu-item-107\"><a href=\"https:\/\/www.celetrix.com\/zh\/recommend-parameters\/\" class=\"elementor-item\">Recommended Parameters<\/a><\/li>\n<li class=\"menu-item menu-item-type-post_type menu-item-object-page menu-item-106\"><a href=\"https:\/\/www.celetrix.com\/zh\/optimization-strategy\/\" class=\"elementor-item\">Optimization Strategy<\/a><\/li>\n<li class=\"menu-item menu-item-type-post_type menu-item-object-page menu-item-104\"><a href=\"https:\/\/www.celetrix.com\/zh\/experimental-overview\/\" class=\"elementor-item\">Experimental Overview<\/a><\/li>\n<li class=\"menu-item menu-item-type-post_type menu-item-object-page menu-item-108\"><a href=\"https:\/\/www.celetrix.com\/zh\/special-cells\/\" class=\"elementor-item\">Special Cells<\/a><\/li>\n<\/ul>\t\t\t<\/nav>\n\t\t\t\t\t\t<nav class=\"elementor-nav-menu--dropdown elementor-nav-menu__container\" aria-hidden=\"true\">\n\t\t\t\t<ul id=\"menu-2-4bb03275\" class=\"elementor-nav-menu sm-vertical\"><li class=\"menu-item menu-item-type-post_type menu-item-object-page menu-item-105\"><a href=\"https:\/\/www.celetrix.com\/zh\/manuals-videos\/\" class=\"elementor-item\" tabindex=\"-1\">Manuals &#038; Videos<\/a><\/li>\n<li class=\"menu-item menu-item-type-post_type menu-item-object-page menu-item-107\"><a href=\"https:\/\/www.celetrix.com\/zh\/recommend-parameters\/\" class=\"elementor-item\" tabindex=\"-1\">Recommended Parameters<\/a><\/li>\n<li class=\"menu-item menu-item-type-post_type menu-item-object-page menu-item-106\"><a href=\"https:\/\/www.celetrix.com\/zh\/optimization-strategy\/\" class=\"elementor-item\" tabindex=\"-1\">Optimization Strategy<\/a><\/li>\n<li class=\"menu-item menu-item-type-post_type menu-item-object-page menu-item-104\"><a href=\"https:\/\/www.celetrix.com\/zh\/experimental-overview\/\" class=\"elementor-item\" tabindex=\"-1\">Experimental Overview<\/a><\/li>\n<li class=\"menu-item menu-item-type-post_type menu-item-object-page menu-item-108\"><a href=\"https:\/\/www.celetrix.com\/zh\/special-cells\/\" class=\"elementor-item\" tabindex=\"-1\">Special Cells<\/a><\/li>\n<\/ul>\t\t\t<\/nav>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<\/div>","protected":false},"excerpt":{"rendered":"<p>Electroporation by Celetrix Page Electroporation Parameters by Cell Type Recommended Celetrix electroporation voltages for primary cells, stem cells and cell lines. Find your cell type below; linked cell types have a full protocol page with data. For cells not listed, start from a cell type of similar size and fine-tune with our optimization guide. Recommended [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"elementor_header_footer","meta":{"om_disable_all_campaigns":false,"_uf_show_specific_survey":0,"_uf_disable_surveys":false,"footnotes":""},"class_list":["post-51","page","type-page","status-publish","hentry"],"aioseo_notices":[],"_links":{"self":[{"href":"https:\/\/www.celetrix.com\/zh\/wp-json\/wp\/v2\/pages\/51","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.celetrix.com\/zh\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/www.celetrix.com\/zh\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/www.celetrix.com\/zh\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.celetrix.com\/zh\/wp-json\/wp\/v2\/comments?post=51"}],"version-history":[{"count":96,"href":"https:\/\/www.celetrix.com\/zh\/wp-json\/wp\/v2\/pages\/51\/revisions"}],"predecessor-version":[{"id":2603,"href":"https:\/\/www.celetrix.com\/zh\/wp-json\/wp\/v2\/pages\/51\/revisions\/2603"}],"wp:attachment":[{"href":"https:\/\/www.celetrix.com\/zh\/wp-json\/wp\/v2\/media?parent=51"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}