{"id":45,"date":"2021-10-28T08:16:33","date_gmt":"2021-10-28T08:16:33","guid":{"rendered":"https:\/\/celetrix.com\/celetrix\/?page_id=45"},"modified":"2023-08-02T07:03:30","modified_gmt":"2023-08-02T07:03:30","slug":"rnp-mrna-sirna-vs-plasmid","status":"publish","type":"page","link":"https:\/\/www.celetrix.com\/zh\/rnp-mrna-sirna-vs-plasmid\/","title":{"rendered":"RNP mRNA siRNA vs Plasmid"},"content":{"rendered":"<div data-elementor-type=\"wp-page\" data-elementor-id=\"45\" class=\"elementor elementor-45\" data-elementor-post-type=\"page\">\n\t\t\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-653e534a elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"653e534a\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-50 elementor-top-column elementor-element elementor-element-41678995\" data-id=\"41678995\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-dff91b4 elementor-widget elementor-widget-bdt-breadcrumbs\" data-id=\"dff91b4\" data-element_type=\"widget\" data-widget_type=\"bdt-breadcrumbs.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<div class='bdt-breadcrumbs-wrapper'><ul class=\"bdt-breadcrumb breadcrumb-align-left\"><li><a href=\"https:\/\/www.celetrix.com\/zh\/\"><span class=\"bdt-breadcrumbs-home-icon\"> <i aria-hidden=\"true\" class=\"fas fa-home\"><\/i> <\/span>Electroporation by Celetrix<\/a><\/li>\n<li><span> \u9875\u9762<\/span><\/li>\n<\/ul><\/div>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-31c71960 elementor-widget elementor-widget-heading\" data-id=\"31c71960\" data-element_type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h2 class=\"elementor-heading-title elementor-size-default\">mRNA super-high-efficiency electroporation<\/h2>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-5ed5f4c0 elementor-widget elementor-widget-text-editor\" data-id=\"5ed5f4c0\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<div class=\"col-sm-6 col-md-4\"><p><img fetchpriority=\"high\" decoding=\"async\" class=\"wp-image-266 alignleft\" src=\"http:\/\/www.celetrix.com\/wp-content\/uploads\/2021\/11\/20180317151555_507.jpg\"  width=\"313\" height=\"530\" srcset=\"https:\/\/www.celetrix.com\/wp-content\/uploads\/2021\/11\/20180317151555_507.jpg 443w, https:\/\/www.celetrix.com\/wp-content\/uploads\/2021\/11\/20180317151555_507-177x300.jpg 177w\" sizes=\"(max-width: 313px) 100vw, 313px\" title=\"RNP mRNA siRNA vs Plasmid\u63d2\u56fe\" alt=\"RNP mRNA siRNA vs Plasmid\u63d2\u56fe\" \/>Many immunotherapy and research applications require transfection of mRNA. The Celetrix electroporation technology can electroporate mRNA at very high efficiency.<\/p><p>The transfection rate of mRNA is usually higher than that of the plasmids. For example, the GFP mRNA can be electroporated to Jurkat cells at a super-high efficiency of 97.5%.<\/p><p>The levels of mRNA expressed proteins among cells are more even than the proteins expressed from plasmids. However, the protein expression level of mRNA is usually lower than strong plasmids. The Celetrix electroporation can help to achieve a relatively high level of protein expression level and a long expression time.<\/p><\/div><div class=\"col-sm-12 col-md-8-5\"><p>siRNA eletroporation is easier than mRNA. It is generally easy to achieve close to 100% siRNA electroporation, but the protein knockdown level is a different biological issue and it may vary according to siRNA selection.<\/p><p>Also the transfection of mRNA will help some special cells such as macrophage related cell lines that tend to die after plasmid electroporation but not mRNA electroporation. For example BFP mRNA can be electroporated to THP-1 cells at a super-high efficiency of 98.31% and there is almost no cell death.<\/p><p><img decoding=\"async\" class=\"alignnone size-full wp-image-267\" src=\"http:\/\/www.celetrix.com\/wp-content\/uploads\/2021\/11\/20180428162057_199.jpg\"  width=\"396\" height=\"232\" srcset=\"https:\/\/www.celetrix.com\/wp-content\/uploads\/2021\/11\/20180428162057_199.jpg 396w, https:\/\/www.celetrix.com\/wp-content\/uploads\/2021\/11\/20180428162057_199-300x176.jpg 300w\" sizes=\"(max-width: 396px) 100vw, 396px\" title=\"RNP mRNA siRNA vs Plasmid\u63d2\u56fe1\" alt=\"RNP mRNA siRNA vs Plasmid\u63d2\u56fe1\" \/><\/p><\/div>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t<div class=\"elementor-column elementor-col-50 elementor-top-column elementor-element elementor-element-6b664763\" data-id=\"6b664763\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-312b21c2 elementor-nav-menu__align-start elementor-nav-menu--dropdown-none elementor-widget elementor-widget-nav-menu\" data-id=\"312b21c2\" data-element_type=\"widget\" data-settings=\"{&quot;layout&quot;:&quot;vertical&quot;,&quot;submenu_icon&quot;:{&quot;value&quot;:&quot;&lt;i class=\\&quot;fas fa-caret-down\\&quot; aria-hidden=\\&quot;true\\&quot;&gt;&lt;\\\/i&gt;&quot;,&quot;library&quot;:&quot;fa-solid&quot;}}\" data-widget_type=\"nav-menu.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t<nav aria-label=\"Menu\" class=\"elementor-nav-menu--main elementor-nav-menu__container elementor-nav-menu--layout-vertical e--pointer-background e--animation-fade\">\n\t\t\t\t<ul id=\"menu-1-312b21c2\" class=\"elementor-nav-menu sm-vertical\"><li class=\"menu-item menu-item-type-post_type menu-item-object-page menu-item-99\"><a href=\"https:\/\/www.celetrix.com\/zh\/electroporation-mechanism\/\" class=\"elementor-item\">Electroporation Mechanism<\/a><\/li>\n<li class=\"menu-item menu-item-type-post_type menu-item-object-page menu-item-100\"><a href=\"https:\/\/www.celetrix.com\/zh\/nucleus-transfection\/\" class=\"elementor-item\">Nucleus Transfection?<\/a><\/li>\n<li class=\"menu-item menu-item-type-post_type menu-item-object-page menu-item-103\"><a href=\"https:\/\/www.celetrix.com\/zh\/vs-cuvette\/\" class=\"elementor-item\">vs Cuvette<\/a><\/li>\n<li class=\"menu-item menu-item-type-post_type menu-item-object-page menu-item-102\"><a href=\"https:\/\/www.celetrix.com\/zh\/vs-capillary\/\" class=\"elementor-item\">vs Capillary<\/a><\/li>\n<li class=\"menu-item menu-item-type-post_type menu-item-object-page menu-item-101\"><a href=\"https:\/\/www.celetrix.com\/zh\/rnp-mrna-sirna-vs-plasmid\/\" class=\"elementor-item\">RNP mRNA siRNA vs Plasmid<\/a><\/li>\n<li class=\"menu-item menu-item-type-post_type menu-item-object-page menu-item-98\"><a href=\"https:\/\/www.celetrix.com\/zh\/celetrix-advantage\/\" class=\"elementor-item\">Celetrix Advantage<\/a><\/li>\n<\/ul>\t\t\t<\/nav>\n\t\t\t\t\t\t<nav class=\"elementor-nav-menu--dropdown elementor-nav-menu__container\" aria-hidden=\"true\">\n\t\t\t\t<ul id=\"menu-2-312b21c2\" class=\"elementor-nav-menu sm-vertical\"><li class=\"menu-item menu-item-type-post_type menu-item-object-page menu-item-99\"><a href=\"https:\/\/www.celetrix.com\/zh\/electroporation-mechanism\/\" class=\"elementor-item\" tabindex=\"-1\">Electroporation Mechanism<\/a><\/li>\n<li class=\"menu-item menu-item-type-post_type menu-item-object-page menu-item-100\"><a href=\"https:\/\/www.celetrix.com\/zh\/nucleus-transfection\/\" class=\"elementor-item\" tabindex=\"-1\">Nucleus Transfection?<\/a><\/li>\n<li class=\"menu-item menu-item-type-post_type menu-item-object-page menu-item-103\"><a href=\"https:\/\/www.celetrix.com\/zh\/vs-cuvette\/\" class=\"elementor-item\" tabindex=\"-1\">vs Cuvette<\/a><\/li>\n<li class=\"menu-item menu-item-type-post_type menu-item-object-page menu-item-102\"><a href=\"https:\/\/www.celetrix.com\/zh\/vs-capillary\/\" class=\"elementor-item\" tabindex=\"-1\">vs Capillary<\/a><\/li>\n<li class=\"menu-item menu-item-type-post_type menu-item-object-page menu-item-101\"><a href=\"https:\/\/www.celetrix.com\/zh\/rnp-mrna-sirna-vs-plasmid\/\" class=\"elementor-item\" tabindex=\"-1\">RNP mRNA siRNA vs Plasmid<\/a><\/li>\n<li class=\"menu-item menu-item-type-post_type menu-item-object-page menu-item-98\"><a href=\"https:\/\/www.celetrix.com\/zh\/celetrix-advantage\/\" class=\"elementor-item\" tabindex=\"-1\">Celetrix Advantage<\/a><\/li>\n<\/ul>\t\t\t<\/nav>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<\/div>","protected":false},"excerpt":{"rendered":"<p>Electroporation by Celetrix Page mRNA super-high-efficiency electroporation Many immunotherapy and research applications require transfection of mRNA. The Celetrix electroporation technology can electroporate mRNA at very high efficiency. The transfection rate of mRNA is usually higher than that of the plasmids. For example, the GFP mRNA can be electroporated to Jurkat cells at a super-high efficiency [&hellip;]<\/p>","protected":false},"author":1,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"elementor_header_footer","meta":{"om_disable_all_campaigns":false,"_uf_show_specific_survey":0,"_uf_disable_surveys":false,"footnotes":""},"class_list":["post-45","page","type-page","status-publish","hentry"],"aioseo_notices":[],"_links":{"self":[{"href":"https:\/\/www.celetrix.com\/zh\/wp-json\/wp\/v2\/pages\/45","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.celetrix.com\/zh\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/www.celetrix.com\/zh\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/www.celetrix.com\/zh\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.celetrix.com\/zh\/wp-json\/wp\/v2\/comments?post=45"}],"version-history":[{"count":5,"href":"https:\/\/www.celetrix.com\/zh\/wp-json\/wp\/v2\/pages\/45\/revisions"}],"predecessor-version":[{"id":1520,"href":"https:\/\/www.celetrix.com\/zh\/wp-json\/wp\/v2\/pages\/45\/revisions\/1520"}],"wp:attachment":[{"href":"https:\/\/www.celetrix.com\/zh\/wp-json\/wp\/v2\/media?parent=45"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}