{"id":43,"date":"2021-10-28T08:16:12","date_gmt":"2021-10-28T08:16:12","guid":{"rendered":"https:\/\/celetrix.com\/celetrix\/?page_id=43"},"modified":"2025-01-20T02:56:29","modified_gmt":"2025-01-20T02:56:29","slug":"vs-capillary","status":"publish","type":"page","link":"https:\/\/www.celetrix.com\/zh\/vs-capillary\/","title":{"rendered":"vs Capillary"},"content":{"rendered":"<div data-elementor-type=\"wp-page\" data-elementor-id=\"43\" class=\"elementor elementor-43\" data-elementor-post-type=\"page\">\n\t\t\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-34ecbd28 elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"34ecbd28\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-50 elementor-top-column elementor-element elementor-element-192ba510\" data-id=\"192ba510\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-7f2fad68 elementor-widget elementor-widget-bdt-breadcrumbs\" data-id=\"7f2fad68\" data-element_type=\"widget\" data-widget_type=\"bdt-breadcrumbs.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<div class='bdt-breadcrumbs-wrapper'><ul class=\"bdt-breadcrumb breadcrumb-align-left\"><li><a href=\"https:\/\/www.celetrix.com\/zh\/\"><span class=\"bdt-breadcrumbs-home-icon\"> <i aria-hidden=\"true\" class=\"fas fa-home\"><\/i> <\/span>Electroporation by Celetrix<\/a><\/li>\n<li><span> \u9875\u9762<\/span><\/li>\n<\/ul><\/div>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-31ea6648 elementor-widget elementor-widget-heading\" data-id=\"31ea6648\" data-element_type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h2 class=\"elementor-heading-title elementor-size-default\">The Celetrix<sup style=\"margin: 0px;padding: 0px\">TM<\/sup> Sealed Tube<\/h2>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-79d48e49 elementor-widget elementor-widget-text-editor\" data-id=\"79d48e49\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p><strong>1. Limitations of capillary electroporation<\/strong><\/p><div class=\"container\"><div class=\"col-sm-12 col-md-8-5\"><img decoding=\"async\" src=\"http:\/\/www.celetrix.com\/wp-content\/uploads\/2021\/11\/20180326165323_118.jpg\" title=\"vs Capillary\u63d2\u56fe\" alt=\"vs Capillary\u63d2\u56fe\" \/>The wall of the capillary is an insulator. When a cell is very close to the capillary wall, a narrow passage between the cell and the capillary wall is formed for the electric current to pass through. This narrow passage is allocated a higher voltage because liquid resistance is inversely proportional to the cross-sectional area. The effective electroporation surface on the cell is also increased. The capillary effect (CE) can be similarly achieved in a non-capillary solution by placing cells at a high concentration allowing them to be each other\u2019s next insulator.<img fetchpriority=\"high\" decoding=\"async\" class=\"wp-image-259 alignright\" src=\"https:\/\/www.celetrix.com\/wp-content\/uploads\/2024\/09\/VS-CAPILLAY-E.jpg\"  width=\"360\" height=\"316\" title=\"vs Capillary\u63d2\u56fe1\" alt=\"vs Capillary\u63d2\u56fe1\" \/><\/div><div class=\"col-sm-12 col-md-5-5\"><img decoding=\"async\" src=\"http:\/\/www.celetrix.com\/wp-content\/uploads\/2021\/11\/20180326165323_118.jpg\" title=\"vs Capillary\u63d2\u56fe\" alt=\"vs Capillary\u63d2\u56fe\" \/>The capillary effect is very sensitive to the distance between the cell and the capillary wall. When a cell is very close to the capillary wall (\u00a0<img decoding=\"async\" src=\"http:\/\/www.celetrix.com\/wp-content\/uploads\/2021\/11\/20180508144953_115.png\"  width=\"14\" height=\"14\" title=\"vs Capillary\u63d2\u56fe2\" alt=\"vs Capillary\u63d2\u56fe2\" \/>\u00a0), capillary effect (CE) is very strong and this cell would need a lower pulse voltage. As the cell-to-wall distance increases (\u00a0<img decoding=\"async\" src=\"http:\/\/www.celetrix.com\/wp-content\/uploads\/2021\/11\/20180508144953_961.png\"  width=\"14\" height=\"14\" title=\"vs Capillary\u63d2\u56fe3\" alt=\"vs Capillary\u63d2\u56fe3\" \/>), capillary effect quickly weakens and this cell would need a higher pulse voltage. When the cell is placed about two cell diameters\u2019 distance from the capillary wall (\u00a0<img loading=\"lazy\" decoding=\"async\" src=\"http:\/\/www.celetrix.com\/wp-content\/uploads\/2021\/11\/20180508144953_883.png\"  width=\"14\" height=\"14\" title=\"vs Capillary\u63d2\u56fe4\" alt=\"vs Capillary\u63d2\u56fe4\" \/>), there is almost zero capillary effect and this cell would require an even higher voltage.<br \/><img decoding=\"async\" src=\"http:\/\/www.celetrix.com\/wp-content\/uploads\/2021\/11\/20180326165323_118.jpg\" title=\"vs Capillary\u63d2\u56fe\" alt=\"vs Capillary\u63d2\u56fe\" \/>Since all cells in a capillary experience the same voltage pulse from a pulse generator, it is not possible for the pulse to be equally suitable for all three areas of cells. Therefore the capillary mechanism is inherently limited.<\/div><\/div><div class=\"col-sm-12 col-md-12\"><strong>The limitations of capillary electrporation:<\/strong><br \/>1) Help is partial and unequal, inherently unsuitable for high efficiency;<br \/>2) Air bubbles not compressed, current disturbance and arcing;<br \/>3) Small volume, inconvenient and high cost with bulk cell samples;<br \/>4) Low power pulse generator, non-scalable.<\/div>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-45c1667a elementor-widget elementor-widget-text-editor\" data-id=\"45c1667a\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p><strong>2. The Celetrix\u2122 Sealed Tube<\/strong><\/p><div class=\"col-sm-12 col-md-12\">1&gt;. Uniform electric field for high efficiency electroporation.<br \/>2&gt;.Very low production of toxic agents and gas bubbles.<br \/>3&gt;. Selection of sample volumes from small 20ul to large 10ml. <br \/>4&gt;. Fast and cost-effective for large samples.<\/div>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-7cbd6d8 elementor-widget elementor-widget-text-editor\" data-id=\"7cbd6d8\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p><strong>3. Advantages of Celetrix\u2122 Tube<\/strong><\/p><p><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-full wp-image-1587\" src=\"https:\/\/www.celetrix.com\/wp-content\/uploads\/2024\/09\/\u7535\u8f6c\u4ea7\u54c1\u5bf9\u6bd4-EN.png\"  width=\"618\" height=\"551\" srcset=\"https:\/\/www.celetrix.com\/wp-content\/uploads\/2024\/09\/\u7535\u8f6c\u4ea7\u54c1\u5bf9\u6bd4-EN.png 618w, https:\/\/www.celetrix.com\/wp-content\/uploads\/2024\/09\/\u7535\u8f6c\u4ea7\u54c1\u5bf9\u6bd4-EN-300x267.png 300w, https:\/\/www.celetrix.com\/wp-content\/uploads\/2024\/09\/\u7535\u8f6c\u4ea7\u54c1\u5bf9\u6bd4-EN-13x12.png 13w\" sizes=\"(max-width: 618px) 100vw, 618px\" title=\"vs Capillary\u63d2\u56fe5\" alt=\"vs Capillary\u63d2\u56fe5\" \/><\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t<div class=\"elementor-column elementor-col-50 elementor-top-column elementor-element elementor-element-139fa02e\" data-id=\"139fa02e\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-499e487f elementor-nav-menu__align-start elementor-nav-menu--dropdown-none elementor-widget elementor-widget-nav-menu\" data-id=\"499e487f\" data-element_type=\"widget\" data-settings=\"{&quot;layout&quot;:&quot;vertical&quot;,&quot;submenu_icon&quot;:{&quot;value&quot;:&quot;&lt;i class=\\&quot;fas fa-caret-down\\&quot; aria-hidden=\\&quot;true\\&quot;&gt;&lt;\\\/i&gt;&quot;,&quot;library&quot;:&quot;fa-solid&quot;}}\" data-widget_type=\"nav-menu.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t<nav aria-label=\"Menu\" class=\"elementor-nav-menu--main elementor-nav-menu__container elementor-nav-menu--layout-vertical e--pointer-background e--animation-fade\">\n\t\t\t\t<ul id=\"menu-1-499e487f\" class=\"elementor-nav-menu sm-vertical\"><li class=\"menu-item menu-item-type-post_type menu-item-object-page menu-item-99\"><a href=\"https:\/\/www.celetrix.com\/zh\/electroporation-mechanism\/\" class=\"elementor-item\">Electroporation Mechanism<\/a><\/li>\n<li class=\"menu-item menu-item-type-post_type menu-item-object-page menu-item-100\"><a href=\"https:\/\/www.celetrix.com\/zh\/nucleus-transfection\/\" class=\"elementor-item\">Nucleus Transfection?<\/a><\/li>\n<li class=\"menu-item menu-item-type-post_type menu-item-object-page menu-item-103\"><a href=\"https:\/\/www.celetrix.com\/zh\/vs-cuvette\/\" class=\"elementor-item\">vs Cuvette<\/a><\/li>\n<li class=\"menu-item menu-item-type-post_type menu-item-object-page menu-item-102\"><a href=\"https:\/\/www.celetrix.com\/zh\/vs-capillary\/\" class=\"elementor-item\">vs Capillary<\/a><\/li>\n<li class=\"menu-item menu-item-type-post_type menu-item-object-page menu-item-101\"><a href=\"https:\/\/www.celetrix.com\/zh\/rnp-mrna-sirna-vs-plasmid\/\" class=\"elementor-item\">RNP mRNA siRNA vs Plasmid<\/a><\/li>\n<li class=\"menu-item menu-item-type-post_type menu-item-object-page menu-item-98\"><a href=\"https:\/\/www.celetrix.com\/zh\/celetrix-advantage\/\" class=\"elementor-item\">Celetrix Advantage<\/a><\/li>\n<\/ul>\t\t\t<\/nav>\n\t\t\t\t\t\t<nav class=\"elementor-nav-menu--dropdown elementor-nav-menu__container\" aria-hidden=\"true\">\n\t\t\t\t<ul id=\"menu-2-499e487f\" class=\"elementor-nav-menu sm-vertical\"><li class=\"menu-item menu-item-type-post_type menu-item-object-page menu-item-99\"><a href=\"https:\/\/www.celetrix.com\/zh\/electroporation-mechanism\/\" class=\"elementor-item\" tabindex=\"-1\">Electroporation Mechanism<\/a><\/li>\n<li class=\"menu-item menu-item-type-post_type menu-item-object-page menu-item-100\"><a href=\"https:\/\/www.celetrix.com\/zh\/nucleus-transfection\/\" class=\"elementor-item\" tabindex=\"-1\">Nucleus Transfection?<\/a><\/li>\n<li class=\"menu-item menu-item-type-post_type menu-item-object-page menu-item-103\"><a href=\"https:\/\/www.celetrix.com\/zh\/vs-cuvette\/\" class=\"elementor-item\" tabindex=\"-1\">vs Cuvette<\/a><\/li>\n<li class=\"menu-item menu-item-type-post_type menu-item-object-page menu-item-102\"><a href=\"https:\/\/www.celetrix.com\/zh\/vs-capillary\/\" class=\"elementor-item\" tabindex=\"-1\">vs Capillary<\/a><\/li>\n<li class=\"menu-item menu-item-type-post_type menu-item-object-page menu-item-101\"><a href=\"https:\/\/www.celetrix.com\/zh\/rnp-mrna-sirna-vs-plasmid\/\" class=\"elementor-item\" tabindex=\"-1\">RNP mRNA siRNA vs Plasmid<\/a><\/li>\n<li class=\"menu-item menu-item-type-post_type menu-item-object-page menu-item-98\"><a href=\"https:\/\/www.celetrix.com\/zh\/celetrix-advantage\/\" class=\"elementor-item\" tabindex=\"-1\">Celetrix Advantage<\/a><\/li>\n<\/ul>\t\t\t<\/nav>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<\/div>","protected":false},"excerpt":{"rendered":"<p>Electroporation by Celetrix Page The CeletrixTM Sealed Tube 1. Limitations of capillary electroporation The wall of the capillary is an insulator. When a cell is very close to the capillary wall, a narrow passage between the cell and the capillary wall is formed for the electric current to pass through. This narrow passage is allocated [&hellip;]<\/p>","protected":false},"author":1,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"elementor_header_footer","meta":{"om_disable_all_campaigns":false,"_uf_show_specific_survey":0,"_uf_disable_surveys":false,"footnotes":""},"class_list":["post-43","page","type-page","status-publish","hentry"],"aioseo_notices":[],"_links":{"self":[{"href":"https:\/\/www.celetrix.com\/zh\/wp-json\/wp\/v2\/pages\/43","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.celetrix.com\/zh\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/www.celetrix.com\/zh\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/www.celetrix.com\/zh\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.celetrix.com\/zh\/wp-json\/wp\/v2\/comments?post=43"}],"version-history":[{"count":29,"href":"https:\/\/www.celetrix.com\/zh\/wp-json\/wp\/v2\/pages\/43\/revisions"}],"predecessor-version":[{"id":1691,"href":"https:\/\/www.celetrix.com\/zh\/wp-json\/wp\/v2\/pages\/43\/revisions\/1691"}],"wp:attachment":[{"href":"https:\/\/www.celetrix.com\/zh\/wp-json\/wp\/v2\/media?parent=43"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}