{"id":33,"date":"2021-10-28T08:15:15","date_gmt":"2021-10-28T08:15:15","guid":{"rendered":"https:\/\/celetrix.com\/celetrix\/?page_id=33"},"modified":"2026-05-25T08:56:24","modified_gmt":"2026-05-25T08:56:24","slug":"antibody-production","status":"publish","type":"page","link":"https:\/\/www.celetrix.com\/zh\/antibody-production\/","title":{"rendered":"Antibody production"},"content":{"rendered":"<div data-elementor-type=\"wp-page\" data-elementor-id=\"33\" class=\"elementor elementor-33\" data-elementor-post-type=\"page\">\n\t\t\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-71077a9 elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"71077a9\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-50 elementor-top-column elementor-element elementor-element-2a1293e5\" data-id=\"2a1293e5\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-6476c386 elementor-widget elementor-widget-bdt-breadcrumbs\" data-id=\"6476c386\" data-element_type=\"widget\" data-widget_type=\"bdt-breadcrumbs.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<div class='bdt-breadcrumbs-wrapper'><ul class=\"bdt-breadcrumb breadcrumb-align-left\"><li><a href=\"https:\/\/www.celetrix.com\/zh\/\"><span class=\"bdt-breadcrumbs-home-icon\"> <i aria-hidden=\"true\" class=\"fas fa-home\"><\/i> <\/span>Electroporation by Celetrix<\/a><\/li>\n<li><span> \u9875\u9762<\/span><\/li>\n<\/ul><\/div>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-e496e21 elementor-widget elementor-widget-heading\" data-id=\"e496e21\" data-element_type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h2 class=\"elementor-heading-title elementor-size-default\">Large scale antibody production<\/h2>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-4985e39d elementor-widget elementor-widget-text-editor\" data-id=\"4985e39d\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p>Model SLT (Single Large Tube) 10ml electroporator can electroporate 2x10E9CHO cells instantly for 400ml Fed-batch culture to reach g\/L antibody expression level in 7 days. Manually processing 5 tubes for 2L Fed-batch culture would take only about 5 minutes.<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<section class=\"elementor-section elementor-inner-section elementor-element elementor-element-4fa083a elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"4fa083a\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-33 elementor-inner-column elementor-element elementor-element-a285533\" data-id=\"a285533\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap\">\n\t\t\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t<div class=\"elementor-column elementor-col-33 elementor-inner-column elementor-element elementor-element-f1037b0\" data-id=\"f1037b0\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-8d569b0 elementor-widget elementor-widget-image\" data-id=\"8d569b0\" data-element_type=\"widget\" data-widget_type=\"image.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<img fetchpriority=\"high\" decoding=\"async\" width=\"400\" height=\"300\" src=\"https:\/\/www.celetrix.com\/wp-content\/uploads\/celetrix-model-slt-large-scale-antibody-production-electroporator.png\" class=\"attachment-large size-large wp-image-1888\" alt=\"Celetrix Model SLT industrial electroporator featuring a unique 10ml single large tube. It allows instant transfection of 2x10E9 CHO cells for large scale antibody production.\" srcset=\"https:\/\/www.celetrix.com\/wp-content\/uploads\/celetrix-model-slt-large-scale-antibody-production-electroporator.png 400w, https:\/\/www.celetrix.com\/wp-content\/uploads\/celetrix-model-slt-large-scale-antibody-production-electroporator-300x225.png 300w, https:\/\/www.celetrix.com\/wp-content\/uploads\/celetrix-model-slt-large-scale-antibody-production-electroporator-16x12.png 16w\" sizes=\"(max-width: 400px) 100vw, 400px\" \/>\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t<div class=\"elementor-column elementor-col-33 elementor-inner-column elementor-element elementor-element-f4015f0\" data-id=\"f4015f0\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap\">\n\t\t\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<div class=\"elementor-element elementor-element-2ffe6c7 elementor-widget elementor-widget-text-editor\" data-id=\"2ffe6c7\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<div>Model SLT also supports 200ul and 1ml electroporation tubes for smaller scale testing. It can also be equipped with cell fusion accessories for hybridoma generation.<\/div><div>Model EX+ electroporator can process 1ml tubes for 2&#215;10<sup>8<\/sup>\u00a0CHO cells for 40ml Fed-batch culture to harvest about 40 mg antibody. It is also suitable for stable pool generation and high expression clone screening.<\/div><div>Compared to flow electroporation devices on the market, single large tubes run much faster and there is no efficiency loss suffered by flow electroporation. Both small scale and large scale Celetrix electroporators can reach 99% efficiency on CHO cells with small plasmids and over 80% efficiency with 11kb larger plasmids.<\/div>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<section class=\"elementor-section elementor-inner-section elementor-element elementor-element-46a90fd elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"46a90fd\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-33 elementor-inner-column elementor-element elementor-element-8e84b8e\" data-id=\"8e84b8e\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap\">\n\t\t\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t<div class=\"elementor-column elementor-col-33 elementor-inner-column elementor-element elementor-element-b93a749\" data-id=\"b93a749\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-8d9be03 elementor-widget elementor-widget-image\" data-id=\"8d9be03\" data-element_type=\"widget\" data-widget_type=\"image.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<img decoding=\"async\" width=\"768\" height=\"457\" src=\"https:\/\/www.celetrix.com\/wp-content\/uploads\/celetrix-cho-cell-6kb-plasmid-transfection-efficiency.jpg\" class=\"attachment-large size-large wp-image-1896\" alt=\"Flow cytometry plot of CHO cells showing over 99 percent transfection efficiency with a 6kb plasmid after Celetrix electroporation.\" srcset=\"https:\/\/www.celetrix.com\/wp-content\/uploads\/celetrix-cho-cell-6kb-plasmid-transfection-efficiency.jpg 768w, https:\/\/www.celetrix.com\/wp-content\/uploads\/celetrix-cho-cell-6kb-plasmid-transfection-efficiency-300x179.jpg 300w, https:\/\/www.celetrix.com\/wp-content\/uploads\/celetrix-cho-cell-6kb-plasmid-transfection-efficiency-18x12.jpg 18w\" sizes=\"(max-width: 768px) 100vw, 768px\" \/>\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-2097caa elementor-widget elementor-widget-image\" data-id=\"2097caa\" data-element_type=\"widget\" data-widget_type=\"image.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<img decoding=\"async\" width=\"768\" height=\"450\" src=\"https:\/\/www.celetrix.com\/wp-content\/uploads\/celetrix-cho-cell-11kb-large-plasmid-transfection-efficiency.png\" class=\"attachment-large size-large wp-image-1911\" alt=\"Flow cytometry plot of CHO cells showing over 80 percent transfection efficiency with an 11kb large plasmid after Celetrix electroporation.\" srcset=\"https:\/\/www.celetrix.com\/wp-content\/uploads\/celetrix-cho-cell-11kb-large-plasmid-transfection-efficiency.png 768w, https:\/\/www.celetrix.com\/wp-content\/uploads\/celetrix-cho-cell-11kb-large-plasmid-transfection-efficiency-300x176.png 300w, https:\/\/www.celetrix.com\/wp-content\/uploads\/celetrix-cho-cell-11kb-large-plasmid-transfection-efficiency-18x12.png 18w\" sizes=\"(max-width: 768px) 100vw, 768px\" \/>\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t<div class=\"elementor-column elementor-col-33 elementor-inner-column elementor-element elementor-element-ee3cf23\" data-id=\"ee3cf23\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap\">\n\t\t\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<div class=\"elementor-element elementor-element-6de8edc elementor-widget elementor-widget-text-editor\" data-id=\"6de8edc\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p>For even larger production scales much more than gram quantity, we recommend the stable pool method. Starting from 10ml electroporation, a stable expression pool can be generated in 10-14 days and it can be frozen and thawed for higher and more reproducible production.\u00a0<\/p><p><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter wp-image-1907\" src=\"https:\/\/www.celetrix.com\/wp-content\/uploads\/celetrix-cho-cell-antibody-production-sds-page-gel-300x221.png\" alt=\"SDS-PAGE gel image demonstrating continuous and high yield antibody production from CHO cells from day 6 to day 14 after Celetrix electroporation.\" width=\"400\" height=\"295\" srcset=\"https:\/\/www.celetrix.com\/wp-content\/uploads\/celetrix-cho-cell-antibody-production-sds-page-gel-300x221.png 300w, https:\/\/www.celetrix.com\/wp-content\/uploads\/celetrix-cho-cell-antibody-production-sds-page-gel-16x12.png 16w, https:\/\/www.celetrix.com\/wp-content\/uploads\/celetrix-cho-cell-antibody-production-sds-page-gel.png 679w\" sizes=\"(max-width: 400px) 100vw, 400px\" \/><\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-dba563f elementor-widget elementor-widget-heading\" data-id=\"dba563f\" data-element_type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h3 class=\"elementor-heading-title elementor-size-default\">Celetrix-SLT 10ml vs Other brand Flow electroporation<\/h3>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-299e49e elementor-widget__width-initial elementor-widget elementor-widget-bdt-table\" data-id=\"299e49e\" data-element_type=\"widget\" data-widget_type=\"bdt-table.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t          <div class=\"bdt-table bdt-overflow-auto bdt-stripe\" id=\"bdt-table-299e49e\">\n              \n              \n              <style>\n    .celetrix-compare-table {\n        width: 100%;\n        border-collapse: collapse;\n        margin-bottom: 15px;\n        table-layout: fixed; \n    .celetrix-compare-table th {\n        font-size: 18px !important;\n        font-weight: bold !important;\n        text-align: left !important;\n        vertical-align: middle !important;\n        padding: 12px 10px;\n        background-color: #f8f9fa; \n    .celetrix-compare-table td {\n        text-align: left !important;\n        vertical-align: middle !important;\n        padding: 10px;\n        border-bottom: 1px solid #dee2e6;\n        word-break: break-word; \n<\/style>\n\n<table class=\"celetrix-compare-table\">\n    <thead>\n        <tr>\n            <th style=\"width: 25% !important;\">Feature<\/th>\n            \n            <th style=\"width: 25% !important;\">Celetrix 10ml tube<\/th>\n            \n            <th style=\"width: 50% !important; white-space: nowrap !important;\">(Other brand) Flow electroporation<\/th>\n        <\/tr>\n    <\/thead>\n    <tbody>\n        <tr>\n            <td>CHO cell number<\/td>\n            <td>2x10E9<\/td>\n            <td>1x10E10 (100ml)<\/td>\n        <\/tr>\n        <tr>\n            <td>Electroporation time<\/td>\n            <td>1 second<\/td>\n            <td>20 minutes<\/td>\n        <\/tr>\n        <tr>\n            <td>HC+LC plasmids<\/td>\n            <td>1 mg<\/td>\n            <td>20 mg<\/td>\n        <\/tr>\n        <tr>\n            <td>Culture volume (pcDNA vector)<\/td>\n            <td>200-400 ml<\/td>\n            <td>2 L<\/td>\n        <\/tr>\n        <tr>\n            <td>Antibody amount<\/td>\n            <td>up to 300 mg<\/td>\n            <td>up to 1 gram<\/td>\n        <\/tr>\n        <tr>\n            <td>Culture volume (Transposon vector)<\/td>\n            <td>1-2 L<\/td>\n            <td>*<\/td>\n        <\/tr>\n        <tr>\n            <td>Antibody amount<\/td>\n            <td>up to 2 gram<\/td>\n            <td>*<\/td>\n        <\/tr>\n        <tr>\n            <td>Sample cost<\/td>\n            <td>Very low<\/td>\n            <td>High<\/td>\n        <\/tr>    \n    <\/tbody>\n<\/table>\n<p style=\"font-style: italic; color: #666; margin-top: 8px;\">*Transposon vectors do not need cumbersome super large scale electroporation.<\/p>\n          <\/div>\n            \t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t<div class=\"elementor-column elementor-col-50 elementor-top-column elementor-element elementor-element-738ec210\" data-id=\"738ec210\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-b562f06 elementor-nav-menu__align-start elementor-nav-menu--dropdown-none elementor-widget elementor-widget-nav-menu\" data-id=\"b562f06\" data-element_type=\"widget\" data-settings=\"{&quot;layout&quot;:&quot;vertical&quot;,&quot;submenu_icon&quot;:{&quot;value&quot;:&quot;&lt;i class=\\&quot;fas fa-caret-down\\&quot; aria-hidden=\\&quot;true\\&quot;&gt;&lt;\\\/i&gt;&quot;,&quot;library&quot;:&quot;fa-solid&quot;}}\" data-widget_type=\"nav-menu.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t<nav aria-label=\"Menu\" class=\"elementor-nav-menu--main elementor-nav-menu__container elementor-nav-menu--layout-vertical e--pointer-background e--animation-fade\">\n\t\t\t\t<ul id=\"menu-1-b562f06\" class=\"elementor-nav-menu sm-vertical\"><li class=\"menu-item menu-item-type-post_type menu-item-object-page menu-item-287\"><a href=\"https:\/\/www.celetrix.com\/zh\/genome-editing\/\" class=\"elementor-item\">Genome Editing<\/a><\/li>\n<li class=\"menu-item menu-item-type-post_type menu-item-object-page menu-item-285\"><a href=\"https:\/\/www.celetrix.com\/zh\/antibody-production\/\" class=\"elementor-item\">Antibody production<\/a><\/li>\n<li class=\"menu-item menu-item-type-post_type menu-item-object-page menu-item-288\"><a href=\"https:\/\/www.celetrix.com\/zh\/stem-cell-therapy\/\" class=\"elementor-item\">Stem cell therapy<\/a><\/li>\n<li class=\"menu-item menu-item-type-post_type menu-item-object-page menu-item-286\"><a href=\"https:\/\/www.celetrix.com\/zh\/car-tcar-nk\/\" class=\"elementor-item\">CAR-T&#038;CAR-NK<\/a><\/li>\n<\/ul>\t\t\t<\/nav>\n\t\t\t\t\t\t<nav class=\"elementor-nav-menu--dropdown elementor-nav-menu__container\" aria-hidden=\"true\">\n\t\t\t\t<ul id=\"menu-2-b562f06\" class=\"elementor-nav-menu sm-vertical\"><li class=\"menu-item menu-item-type-post_type menu-item-object-page menu-item-287\"><a href=\"https:\/\/www.celetrix.com\/zh\/genome-editing\/\" class=\"elementor-item\" tabindex=\"-1\">Genome Editing<\/a><\/li>\n<li class=\"menu-item menu-item-type-post_type menu-item-object-page menu-item-285\"><a href=\"https:\/\/www.celetrix.com\/zh\/antibody-production\/\" class=\"elementor-item\" tabindex=\"-1\">Antibody production<\/a><\/li>\n<li class=\"menu-item menu-item-type-post_type menu-item-object-page menu-item-288\"><a href=\"https:\/\/www.celetrix.com\/zh\/stem-cell-therapy\/\" class=\"elementor-item\" tabindex=\"-1\">Stem cell therapy<\/a><\/li>\n<li class=\"menu-item menu-item-type-post_type menu-item-object-page menu-item-286\"><a href=\"https:\/\/www.celetrix.com\/zh\/car-tcar-nk\/\" class=\"elementor-item\" tabindex=\"-1\">CAR-T&#038;CAR-NK<\/a><\/li>\n<\/ul>\t\t\t<\/nav>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<\/div>","protected":false},"excerpt":{"rendered":"<p>Electroporation by Celetrix Page Large scale antibody production Model SLT (Single Large Tube) 10ml electroporator can electroporate 2x10E9CHO cells instantly for 400ml Fed-batch culture to reach g\/L antibody expression level in 7 days. Manually processing 5 tubes for 2L Fed-batch culture would take only about 5 minutes. Model SLT also supports 200ul and 1ml electroporation [&hellip;]<\/p>","protected":false},"author":1,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"elementor_header_footer","meta":{"om_disable_all_campaigns":false,"_uf_show_specific_survey":0,"_uf_disable_surveys":false,"footnotes":""},"class_list":["post-33","page","type-page","status-publish","hentry"],"aioseo_notices":[],"_links":{"self":[{"href":"https:\/\/www.celetrix.com\/zh\/wp-json\/wp\/v2\/pages\/33","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.celetrix.com\/zh\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/www.celetrix.com\/zh\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/www.celetrix.com\/zh\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.celetrix.com\/zh\/wp-json\/wp\/v2\/comments?post=33"}],"version-history":[{"count":104,"href":"https:\/\/www.celetrix.com\/zh\/wp-json\/wp\/v2\/pages\/33\/revisions"}],"predecessor-version":[{"id":1918,"href":"https:\/\/www.celetrix.com\/zh\/wp-json\/wp\/v2\/pages\/33\/revisions\/1918"}],"wp:attachment":[{"href":"https:\/\/www.celetrix.com\/zh\/wp-json\/wp\/v2\/media?parent=33"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}