{"id":33,"date":"2021-10-28T08:15:15","date_gmt":"2021-10-28T08:15:15","guid":{"rendered":"https:\/\/celetrix.com\/celetrix\/?page_id=33"},"modified":"2026-10-22T09:52:35","modified_gmt":"2026-10-22T09:52:35","slug":"antibody-production","status":"publish","type":"page","link":"https:\/\/www.celetrix.com\/zh\/antibody-production\/","title":{"rendered":"Antibody production"},"content":{"rendered":"\t\t<div data-elementor-type=\"wp-page\" data-elementor-id=\"33\" class=\"elementor elementor-33\" data-elementor-post-type=\"page\">\n\t\t\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-75611b5 elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"75611b5\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-50 elementor-top-column elementor-element elementor-element-bec41fe\" data-id=\"bec41fe\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-71451f6e elementor-widget elementor-widget-bdt-breadcrumbs\" data-id=\"71451f6e\" data-element_type=\"widget\" data-widget_type=\"bdt-breadcrumbs.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<div class='bdt-breadcrumbs-wrapper'><ul class=\"bdt-breadcrumb breadcrumb-align-left  \"><li><a href=\"https:\/\/www.celetrix.com\/zh\/\"><span class=\"bdt-breadcrumbs-home-icon\"> <i aria-hidden=\"true\" class=\"fas fa-home\"><\/i> <\/span>Electroporation by Celetrix<\/a><\/li>\n<li><span> \u9875\u9762<\/span><\/li>\n<\/ul><\/div>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-26d5778a elementor-widget elementor-widget-heading\" data-id=\"26d5778a\" data-element_type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h1 class=\"elementor-heading-title elementor-size-default\">Large-Scale Antibody Production in CHO Cells<\/h1>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-18375751 elementor-widget elementor-widget-text-editor\" data-id=\"18375751\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p>In-scale transient expression: electroporate 2\u00d710<sup>9<\/sup> CHO cells in a single 10 ml tube and harvest g\/L antibody titers in 7 days.<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<section class=\"elementor-section elementor-inner-section elementor-element elementor-element-631636c2 elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"631636c2\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-no\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-25 elementor-inner-column elementor-element elementor-element-7179d59e\" data-id=\"7179d59e\" data-element_type=\"column\" data-settings=\"{&quot;background_background&quot;:&quot;classic&quot;}\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-5490df7b elementor-widget elementor-widget-heading\" data-id=\"5490df7b\" data-element_type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<div class=\"elementor-heading-title elementor-size-default\">2\u00d710<sup>9<\/sup><\/div>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-766c2946 elementor-widget elementor-widget-text-editor\" data-id=\"766c2946\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p style=\"text-align:center;font-size:13px;line-height:1.4;margin:6px 0 0;\">CHO cells per 10 ml tube<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t<div class=\"elementor-column elementor-col-25 elementor-inner-column elementor-element elementor-element-32bc4576\" data-id=\"32bc4576\" data-element_type=\"column\" data-settings=\"{&quot;background_background&quot;:&quot;classic&quot;}\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-14792ba elementor-widget elementor-widget-heading\" data-id=\"14792ba\" data-element_type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<div class=\"elementor-heading-title elementor-size-default\">400 ml<\/div>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-7d1b7635 elementor-widget elementor-widget-text-editor\" data-id=\"7d1b7635\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p style=\"text-align:center;font-size:13px;line-height:1.4;margin:6px 0 0;\">Fed-batch culture<br>(up to 1 L with transposons)<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t<div class=\"elementor-column elementor-col-25 elementor-inner-column elementor-element elementor-element-668c623f\" data-id=\"668c623f\" data-element_type=\"column\" data-settings=\"{&quot;background_background&quot;:&quot;classic&quot;}\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-5c5bad6e elementor-widget elementor-widget-heading\" data-id=\"5c5bad6e\" data-element_type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<div class=\"elementor-heading-title elementor-size-default\">99%<\/div>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-4fc38296 elementor-widget elementor-widget-text-editor\" data-id=\"4fc38296\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p style=\"text-align:center;font-size:13px;line-height:1.4;margin:6px 0 0;\">Efficiency with<br>6 kb plasmid<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t<div class=\"elementor-column elementor-col-25 elementor-inner-column elementor-element elementor-element-6a82118b\" data-id=\"6a82118b\" data-element_type=\"column\" data-settings=\"{&quot;background_background&quot;:&quot;classic&quot;}\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-5c2ccd3c elementor-widget elementor-widget-heading\" data-id=\"5c2ccd3c\" data-element_type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<div class=\"elementor-heading-title elementor-size-default\">7 days<\/div>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-20f14ba3 elementor-widget elementor-widget-text-editor\" data-id=\"20f14ba3\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p style=\"text-align:center;font-size:13px;line-height:1.4;margin:6px 0 0;\">To g\/L antibody titers<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<div class=\"elementor-element elementor-element-55aaf735 elementor-widget elementor-widget-heading\" data-id=\"55aaf735\" data-element_type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h2 class=\"elementor-heading-title elementor-size-default\">One Instrument, Every Scale<\/h2>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-60db0d5 elementor-widget elementor-widget-text-editor\" data-id=\"60db0d5\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p>Model SLT handles 200 \u00b5l to 10 ml tubes, from stable cell line generation to gram-scale production. It also supports cell fusion accessories for hybridoma generation.<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<section class=\"elementor-section elementor-inner-section elementor-element elementor-element-199855b8 elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"199855b8\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-33 elementor-inner-column elementor-element elementor-element-4a16b7d8\" data-id=\"4a16b7d8\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap\">\n\t\t\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t<div class=\"elementor-column elementor-col-33 elementor-inner-column elementor-element elementor-element-55daab\" data-id=\"55daab\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-2e93b3b8 elementor-widget elementor-widget-image\" data-id=\"2e93b3b8\" data-element_type=\"widget\" data-widget_type=\"image.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<img fetchpriority=\"high\" decoding=\"async\" width=\"400\" height=\"300\" src=\"https:\/\/www.celetrix.com\/wp-content\/uploads\/celetrix-model-slt-large-scale-antibody-production-electroporator.png\" class=\"attachment-large size-large wp-image-1888\" alt=\"Celetrix Model SLT industrial electroporator featuring a unique 10ml single large tube. It allows instant transfection of 2x10E9 CHO cells for large scale antibody production.\" srcset=\"https:\/\/www.celetrix.com\/wp-content\/uploads\/celetrix-model-slt-large-scale-antibody-production-electroporator.png 400w, https:\/\/www.celetrix.com\/wp-content\/uploads\/celetrix-model-slt-large-scale-antibody-production-electroporator-300x225.png 300w, https:\/\/www.celetrix.com\/wp-content\/uploads\/celetrix-model-slt-large-scale-antibody-production-electroporator-16x12.png 16w\" sizes=\"(max-width: 400px) 100vw, 400px\" \/>\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t<div class=\"elementor-column elementor-col-33 elementor-inner-column elementor-element elementor-element-2e7f8596\" data-id=\"2e7f8596\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap\">\n\t\t\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<div class=\"elementor-element elementor-element-6a6e4120 elementor-widget elementor-widget-text-editor\" data-id=\"6a6e4120\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<table class=\"cx-scale-table\" style=\"width:100%;border-collapse:collapse;table-layout:fixed;font-family:Montserrat,sans-serif;font-size:16px;\"><thead><tr><th style=\"text-align:left;vertical-align:middle;padding:14px 16px;background:#f8f9fa;border-bottom:2px solid #dee2e6;width:22%;\">Tube<\/th><th style=\"text-align:left;vertical-align:middle;padding:14px 16px;background:#f8f9fa;border-bottom:2px solid #dee2e6;width:26%;\">CHO cells<\/th><th style=\"text-align:left;vertical-align:middle;padding:14px 16px;background:#f8f9fa;border-bottom:2px solid #dee2e6;\">Typical use<\/th><\/tr><\/thead><tbody><tr><td style=\"text-align:left;vertical-align:middle;padding:14px 16px;border-bottom:1px solid #dee2e6;overflow-wrap:anywhere;\">200 \u00b5l<\/td><td style=\"text-align:left;vertical-align:middle;padding:14px 16px;border-bottom:1px solid #dee2e6;overflow-wrap:anywhere;\">2\u00d710<sup>7<\/sup><\/td><td style=\"text-align:left;vertical-align:middle;padding:14px 16px;border-bottom:1px solid #dee2e6;overflow-wrap:anywhere;\">Stable cell line generation<\/td><\/tr><tr><td style=\"text-align:left;vertical-align:middle;padding:14px 16px;border-bottom:1px solid #dee2e6;overflow-wrap:anywhere;\">1 ml<\/td><td style=\"text-align:left;vertical-align:middle;padding:14px 16px;border-bottom:1px solid #dee2e6;overflow-wrap:anywhere;\">2\u00d710<sup>8<\/sup><\/td><td style=\"text-align:left;vertical-align:middle;padding:14px 16px;border-bottom:1px solid #dee2e6;overflow-wrap:anywhere;\">20\u201340 ml fed-batch, condition screening<\/td><\/tr><tr><td style=\"text-align:left;vertical-align:middle;padding:14px 16px;border-bottom:1px solid #dee2e6;overflow-wrap:anywhere;\">10 ml<\/td><td style=\"text-align:left;vertical-align:middle;padding:14px 16px;border-bottom:1px solid #dee2e6;overflow-wrap:anywhere;\">2\u00d710<sup>9<\/sup><\/td><td style=\"text-align:left;vertical-align:middle;padding:14px 16px;border-bottom:1px solid #dee2e6;overflow-wrap:anywhere;\">400 ml fed-batch, or 1 L with transposon vectors<\/td><\/tr><\/tbody><\/table>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<section class=\"elementor-section elementor-inner-section elementor-element elementor-element-47a333c9 elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"47a333c9\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-33 elementor-inner-column elementor-element elementor-element-2b4ffe87\" data-id=\"2b4ffe87\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap\">\n\t\t\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t<div class=\"elementor-column elementor-col-33 elementor-inner-column elementor-element elementor-element-79f4d34b\" data-id=\"79f4d34b\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-305cb9cb elementor-widget elementor-widget-image\" data-id=\"305cb9cb\" data-element_type=\"widget\" data-widget_type=\"image.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<img decoding=\"async\" width=\"768\" height=\"457\" src=\"https:\/\/www.celetrix.com\/wp-content\/uploads\/celetrix-cho-cell-6kb-plasmid-transfection-efficiency.jpg\" class=\"attachment-large size-large wp-image-1896\" alt=\"Flow cytometry plot of CHO cells showing over 99 percent transfection efficiency with a 6kb plasmid after Celetrix electroporation.\" srcset=\"https:\/\/www.celetrix.com\/wp-content\/uploads\/celetrix-cho-cell-6kb-plasmid-transfection-efficiency.jpg 768w, https:\/\/www.celetrix.com\/wp-content\/uploads\/celetrix-cho-cell-6kb-plasmid-transfection-efficiency-300x179.jpg 300w, https:\/\/www.celetrix.com\/wp-content\/uploads\/celetrix-cho-cell-6kb-plasmid-transfection-efficiency-18x12.jpg 18w\" sizes=\"(max-width: 768px) 100vw, 768px\" \/>\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-6f8a518 elementor-widget elementor-widget-image\" data-id=\"6f8a518\" data-element_type=\"widget\" data-widget_type=\"image.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<img decoding=\"async\" width=\"768\" height=\"450\" src=\"https:\/\/www.celetrix.com\/wp-content\/uploads\/celetrix-cho-cell-11kb-large-plasmid-transfection-efficiency.png\" class=\"attachment-large size-large wp-image-1911\" alt=\"Flow cytometry plot of CHO cells showing over 80 percent transfection efficiency with an 11kb large plasmid after Celetrix electroporation.\" srcset=\"https:\/\/www.celetrix.com\/wp-content\/uploads\/celetrix-cho-cell-11kb-large-plasmid-transfection-efficiency.png 768w, https:\/\/www.celetrix.com\/wp-content\/uploads\/celetrix-cho-cell-11kb-large-plasmid-transfection-efficiency-300x176.png 300w, https:\/\/www.celetrix.com\/wp-content\/uploads\/celetrix-cho-cell-11kb-large-plasmid-transfection-efficiency-18x12.png 18w\" sizes=\"(max-width: 768px) 100vw, 768px\" \/>\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t<div class=\"elementor-column elementor-col-33 elementor-inner-column elementor-element elementor-element-62669660\" data-id=\"62669660\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap\">\n\t\t\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<div class=\"elementor-element elementor-element-54c1d0ce elementor-widget elementor-widget-text-editor\" data-id=\"54c1d0ce\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p><strong>Need more than gram quantities?<\/strong> Generate a stable expression pool from a single 10 ml electroporation in 10\u201314 days. Freeze it and repeat production anytime with consistent yields.<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-18eacb2e elementor-widget elementor-widget-heading\" data-id=\"18eacb2e\" data-element_type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h2 class=\"elementor-heading-title elementor-size-default\">Fed-Batch Workflow<\/h2>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-12545072 elementor-widget elementor-widget-text-editor\" data-id=\"12545072\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<div style=\"display:flex;flex-wrap:wrap;gap:8px;\"><div style=\"flex:1 1 120px;border-top:3px solid #0078B5;background:#F5F8FB;padding:10px 12px;\"><strong style=\"color:#0078B5;\">Day 0<\/strong><br>Electroporate and seed at ~10\u00d710<sup>6<\/sup> cells\/ml, 37 \u00b0C<\/div><div style=\"flex:1 1 120px;border-top:3px solid #0078B5;background:#F5F8FB;padding:10px 12px;\"><strong style=\"color:#0078B5;\">Day 1<\/strong><br>Feed #1, shift to 32 \u00b0C<\/div><div style=\"flex:1 1 120px;border-top:3px solid #0078B5;background:#F5F8FB;padding:10px 12px;\"><strong style=\"color:#0078B5;\">Day 3<\/strong><br>Feed #2<\/div><div style=\"flex:1 1 120px;border-top:3px solid #0078B5;background:#F5F8FB;padding:10px 12px;\"><strong style=\"color:#0078B5;\">Day 5<\/strong><br>Feed #3<\/div><div style=\"flex:1 1 120px;border-top:3px solid #0078B5;background:#F5F8FB;padding:10px 12px;\"><strong style=\"color:#0078B5;\">Day 7<\/strong><br>Harvest, or keep feeding to Day 10\u201312<\/div><\/div><p style=\"margin:10px 0 0;font-style:italic;\">Harvest when viability drops from ~95% to ~75%.<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-533c9147 elementor-widget elementor-widget-image\" data-id=\"533c9147\" data-element_type=\"widget\" data-widget_type=\"image.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t\t\t\t<figure class=\"wp-caption\">\n\t\t\t\t\t\t\t\t\t\t<img loading=\"lazy\" decoding=\"async\" width=\"679\" height=\"501\" src=\"https:\/\/www.celetrix.com\/wp-content\/uploads\/celetrix-cho-cell-antibody-production-sds-page-gel.png\" class=\"attachment-full size-full wp-image-1907\" alt=\"SDS-PAGE gel image demonstrating continuous and high yield antibody production from CHO cells from day 6 to day 14 after Celetrix electroporation.\" srcset=\"https:\/\/www.celetrix.com\/wp-content\/uploads\/celetrix-cho-cell-antibody-production-sds-page-gel.png 679w, https:\/\/www.celetrix.com\/wp-content\/uploads\/celetrix-cho-cell-antibody-production-sds-page-gel-300x221.png 300w, https:\/\/www.celetrix.com\/wp-content\/uploads\/celetrix-cho-cell-antibody-production-sds-page-gel-16x12.png 16w\" sizes=\"(max-width: 679px) 100vw, 679px\" \/>\t\t\t\t\t\t\t\t\t\t\t<figcaption class=\"widget-image-caption wp-caption-text\">SDS-PAGE: continuous antibody expression in CHO cells from Day 6 to Day 14 after electroporation<\/figcaption>\n\t\t\t\t\t\t\t\t\t\t<\/figure>\n\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-1f53932e elementor-widget elementor-widget-text-editor\" data-id=\"1f53932e\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<div style=\"background:#F5F8FB;border-left:4px solid #0078B5;padding:14px 18px;margin-top:16px;\"><p style=\"margin:0 0 6px;font-weight:700;color:#0078B5;\">Tips for higher titers<\/p><ul style=\"margin:0;padding-left:18px;\"><li>Seed at 50\u201375% of your medium\u2019s maximal cell density<\/li><li>Culture at 32 \u00b0C after Day 1 for better cell survival<\/li><li>Add 1 mM sodium butyrate for up to 2\u00d7 protein expression<\/li><li>Use transposon (PiggyBac, Sleeping Beauty) or EBNA1-based vectors for even higher yields<\/li><li>Electroporate at room temperature with ~100 \u00b5g\/ml plasmid DNA<\/li><\/ul><\/div>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-6ec89eb4 elementor-widget elementor-widget-heading\" data-id=\"6ec89eb4\" data-element_type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h2 class=\"elementor-heading-title elementor-size-default\">Celetrix SLT vs Flow Electroporation<\/h2>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-36656270 elementor-widget__width-initial elementor-widget elementor-widget-bdt-table\" data-id=\"36656270\" data-element_type=\"widget\" data-widget_type=\"bdt-table.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t          <div class=\"bdt-table bdt-overflow-auto bdt-stripe\" id=\"bdt-table-36656270\">\n              \n              \n              <style>\n    .celetrix-compare-table {\n        width: 100%;\n        border-collapse: collapse;\n        margin-bottom: 15px;\n        table-layout: fixed; \n    .celetrix-compare-table th {\n        font-size: 18px !important;\n        font-weight: bold !important;\n        text-align: left !important;\n        vertical-align: middle !important;\n        padding: 12px 10px;\n        background-color: #f8f9fa; \n    .celetrix-compare-table td {\n        text-align: left !important;\n        vertical-align: middle !important;\n        padding: 10px;\n        border-bottom: 1px solid #dee2e6;\n        word-break: break-word; \n<\/style>\n\n<table class=\"celetrix-compare-table\">\n    <thead>\n        <tr>\n            <th>Feature<\/th>\n            \n            <th>Celetrix 10ml tube<\/th>\n            \n            <th>(Other brand) Flow electroporation<\/th>\n        <\/tr>\n    <\/thead>\n    <tbody>\n        <tr>\n            <td>CHO cell number<\/td>\n            <td>2\u00d710<sup>9<\/sup><\/td>\n            <td>1\u00d710<sup>10<\/sup> (100&nbsp;ml)<\/td>\n        <\/tr>\n        <tr>\n            <td>Processing time<\/td>\n            <td>1 second (single pulse)<\/td>\n            <td>20 minutes<\/td>\n        <\/tr>\n        <tr>\n            <td>HC+LC plasmids<\/td>\n            <td>1 mg<\/td>\n            <td>20 mg<\/td>\n        <\/tr>\n        <tr>\n            <td>Culture volume (pcDNA vector)<\/td>\n            <td>200-400 ml<\/td>\n            <td>2 L<\/td>\n        <\/tr>\n        <tr>\n            <td>Antibody amount<\/td>\n            <td>up to 300 mg<\/td>\n            <td>up to 1 gram<\/td>\n        <\/tr>\n        <tr>\n            <td>Culture volume (Transposon vector)<\/td>\n            <td>1-2 L<\/td>\n            <td>*<\/td>\n        <\/tr>\n        <tr>\n            <td>Antibody amount<\/td>\n            <td>up to 2 gram<\/td>\n            <td>*<\/td>\n        <\/tr>\n        <tr>\n            <td>Sample cost<\/td>\n            <td>Very low<\/td>\n            <td>High<\/td>\n        <\/tr>    \n    <\/tbody>\n<\/table>\n<p style=\"font-style: italic; color: #666; margin-top: 8px;\">*Transposon vectors do not need cumbersome super large scale electroporation.<\/p>\n          <\/div>\n            \t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-f88af2a elementor-widget elementor-widget-heading\" data-id=\"f88af2a\" data-element_type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h2 class=\"elementor-heading-title elementor-size-default\">CHO Transient Antibody Production FAQs<\/h2>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-1bc44521 elementor-widget elementor-widget-accordion\" data-id=\"1bc44521\" data-element_type=\"widget\" data-widget_type=\"accordion.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t<div class=\"elementor-accordion\">\n\t\t\t\t\t\t\t<div class=\"elementor-accordion-item\">\n\t\t\t\t\t<div id=\"elementor-tab-title-4651\" class=\"elementor-tab-title\" data-tab=\"1\" role=\"button\" aria-controls=\"elementor-tab-content-4651\" aria-expanded=\"false\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t<span class=\"elementor-accordion-icon elementor-accordion-icon-left\" aria-hidden=\"true\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<span class=\"elementor-accordion-icon-closed\"><svg class=\"e-font-icon-svg e-fas-plus\" viewBox=\"0 0 448 512\" xmlns=\"http:\/\/www.w3.org\/2000\/svg\"><path d=\"M416 208H272V64c0-17.67-14.33-32-32-32h-32c-17.67 0-32 14.33-32 32v144H32c-17.67 0-32 14.33-32 32v32c0 17.67 14.33 32 32 32h144v144c0 17.67 14.33 32 32 32h32c17.67 0 32-14.33 32-32V304h144c17.67 0 32-14.33 32-32v-32c0-17.67-14.33-32-32-32z\"><\/path><\/svg><\/span>\n\t\t\t\t\t\t\t\t<span class=\"elementor-accordion-icon-opened\"><svg class=\"e-font-icon-svg e-fas-minus\" viewBox=\"0 0 448 512\" xmlns=\"http:\/\/www.w3.org\/2000\/svg\"><path d=\"M416 208H32c-17.67 0-32 14.33-32 32v32c0 17.67 14.33 32 32 32h384c17.67 0 32-14.33 32-32v-32c0-17.67-14.33-32-32-32z\"><\/path><\/svg><\/span>\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/span>\n\t\t\t\t\t\t\t\t\t\t\t\t<a class=\"elementor-accordion-title\" tabindex=\"0\">Which cell lines work for large-scale transient expression by electroporation?<\/a>\n\t\t\t\t\t<\/div>\n\t\t\t\t\t<div id=\"elementor-tab-content-4651\" class=\"elementor-tab-content elementor-clearfix\" data-tab=\"1\" role=\"region\" aria-labelledby=\"elementor-tab-title-4651\"><p>Celetrix Model SLT electroporates CHO-K1, CHO-S, 293F and Vero cells, as well as stimulated T and NK cells. For unstimulated PBMCs, use Celetrix Model LE+ or EX+.<\/p><\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t\t\t<div class=\"elementor-accordion-item\">\n\t\t\t\t\t<div id=\"elementor-tab-title-4652\" class=\"elementor-tab-title\" data-tab=\"2\" role=\"button\" aria-controls=\"elementor-tab-content-4652\" aria-expanded=\"false\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t<span class=\"elementor-accordion-icon elementor-accordion-icon-left\" aria-hidden=\"true\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<span class=\"elementor-accordion-icon-closed\"><svg class=\"e-font-icon-svg e-fas-plus\" viewBox=\"0 0 448 512\" xmlns=\"http:\/\/www.w3.org\/2000\/svg\"><path d=\"M416 208H272V64c0-17.67-14.33-32-32-32h-32c-17.67 0-32 14.33-32 32v144H32c-17.67 0-32 14.33-32 32v32c0 17.67 14.33 32 32 32h144v144c0 17.67 14.33 32 32 32h32c17.67 0 32-14.33 32-32V304h144c17.67 0 32-14.33 32-32v-32c0-17.67-14.33-32-32-32z\"><\/path><\/svg><\/span>\n\t\t\t\t\t\t\t\t<span class=\"elementor-accordion-icon-opened\"><svg class=\"e-font-icon-svg e-fas-minus\" viewBox=\"0 0 448 512\" xmlns=\"http:\/\/www.w3.org\/2000\/svg\"><path d=\"M416 208H32c-17.67 0-32 14.33-32 32v32c0 17.67 14.33 32 32 32h384c17.67 0 32-14.33 32-32v-32c0-17.67-14.33-32-32-32z\"><\/path><\/svg><\/span>\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/span>\n\t\t\t\t\t\t\t\t\t\t\t\t<a class=\"elementor-accordion-title\" tabindex=\"0\">How can I scale up transient antibody production in CHO cells?<\/a>\n\t\t\t\t\t<\/div>\n\t\t\t\t\t<div id=\"elementor-tab-content-4652\" class=\"elementor-tab-content elementor-clearfix\" data-tab=\"2\" role=\"region\" aria-labelledby=\"elementor-tab-title-4652\"><p>Celetrix Model SLT electroporates 2\u00d710<sup>9<\/sup> CHO cells in a single 10 ml tube for a 400 ml fed-batch culture (up to 1 L with transposons), reaching g\/L antibody titers in 7 days.<\/p><\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t\t\t<div class=\"elementor-accordion-item\">\n\t\t\t\t\t<div id=\"elementor-tab-title-4653\" class=\"elementor-tab-title\" data-tab=\"3\" role=\"button\" aria-controls=\"elementor-tab-content-4653\" aria-expanded=\"false\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t<span class=\"elementor-accordion-icon elementor-accordion-icon-left\" aria-hidden=\"true\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<span class=\"elementor-accordion-icon-closed\"><svg class=\"e-font-icon-svg e-fas-plus\" viewBox=\"0 0 448 512\" xmlns=\"http:\/\/www.w3.org\/2000\/svg\"><path d=\"M416 208H272V64c0-17.67-14.33-32-32-32h-32c-17.67 0-32 14.33-32 32v144H32c-17.67 0-32 14.33-32 32v32c0 17.67 14.33 32 32 32h144v144c0 17.67 14.33 32 32 32h32c17.67 0 32-14.33 32-32V304h144c17.67 0 32-14.33 32-32v-32c0-17.67-14.33-32-32-32z\"><\/path><\/svg><\/span>\n\t\t\t\t\t\t\t\t<span class=\"elementor-accordion-icon-opened\"><svg class=\"e-font-icon-svg e-fas-minus\" viewBox=\"0 0 448 512\" xmlns=\"http:\/\/www.w3.org\/2000\/svg\"><path d=\"M416 208H32c-17.67 0-32 14.33-32 32v32c0 17.67 14.33 32 32 32h384c17.67 0 32-14.33 32-32v-32c0-17.67-14.33-32-32-32z\"><\/path><\/svg><\/span>\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/span>\n\t\t\t\t\t\t\t\t\t\t\t\t<a class=\"elementor-accordion-title\" tabindex=\"0\">Do I need a special medium for CHO transient transfection?<\/a>\n\t\t\t\t\t<\/div>\n\t\t\t\t\t<div id=\"elementor-tab-content-4653\" class=\"elementor-tab-content elementor-clearfix\" data-tab=\"3\" role=\"region\" aria-labelledby=\"elementor-tab-title-4653\"><p>No. With Celetrix electroporation, medium choice is less critical than with chemical transfection. Choose a medium that supports high cell density and long culture.<\/p><\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t\t\t<div class=\"elementor-accordion-item\">\n\t\t\t\t\t<div id=\"elementor-tab-title-4654\" class=\"elementor-tab-title\" data-tab=\"4\" role=\"button\" aria-controls=\"elementor-tab-content-4654\" aria-expanded=\"false\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t<span class=\"elementor-accordion-icon elementor-accordion-icon-left\" aria-hidden=\"true\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<span class=\"elementor-accordion-icon-closed\"><svg class=\"e-font-icon-svg e-fas-plus\" viewBox=\"0 0 448 512\" xmlns=\"http:\/\/www.w3.org\/2000\/svg\"><path d=\"M416 208H272V64c0-17.67-14.33-32-32-32h-32c-17.67 0-32 14.33-32 32v144H32c-17.67 0-32 14.33-32 32v32c0 17.67 14.33 32 32 32h144v144c0 17.67 14.33 32 32 32h32c17.67 0 32-14.33 32-32V304h144c17.67 0 32-14.33 32-32v-32c0-17.67-14.33-32-32-32z\"><\/path><\/svg><\/span>\n\t\t\t\t\t\t\t\t<span class=\"elementor-accordion-icon-opened\"><svg class=\"e-font-icon-svg e-fas-minus\" viewBox=\"0 0 448 512\" xmlns=\"http:\/\/www.w3.org\/2000\/svg\"><path d=\"M416 208H32c-17.67 0-32 14.33-32 32v32c0 17.67 14.33 32 32 32h384c17.67 0 32-14.33 32-32v-32c0-17.67-14.33-32-32-32z\"><\/path><\/svg><\/span>\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/span>\n\t\t\t\t\t\t\t\t\t\t\t\t<a class=\"elementor-accordion-title\" tabindex=\"0\">How much plasmid DNA is needed for CHO transient transfection?<\/a>\n\t\t\t\t\t<\/div>\n\t\t\t\t\t<div id=\"elementor-tab-content-4654\" class=\"elementor-tab-content elementor-clearfix\" data-tab=\"4\" role=\"region\" aria-labelledby=\"elementor-tab-title-4654\"><p>For Celetrix electroporation, use 50\u2013200 \u00b5g\/ml plasmid DNA, typically 100 \u00b5g\/ml, about 1 mg per 10 ml tube.<\/p><\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t\t\t<div class=\"elementor-accordion-item\">\n\t\t\t\t\t<div id=\"elementor-tab-title-4655\" class=\"elementor-tab-title\" data-tab=\"5\" role=\"button\" aria-controls=\"elementor-tab-content-4655\" aria-expanded=\"false\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t<span class=\"elementor-accordion-icon elementor-accordion-icon-left\" aria-hidden=\"true\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<span class=\"elementor-accordion-icon-closed\"><svg class=\"e-font-icon-svg e-fas-plus\" viewBox=\"0 0 448 512\" xmlns=\"http:\/\/www.w3.org\/2000\/svg\"><path d=\"M416 208H272V64c0-17.67-14.33-32-32-32h-32c-17.67 0-32 14.33-32 32v144H32c-17.67 0-32 14.33-32 32v32c0 17.67 14.33 32 32 32h144v144c0 17.67 14.33 32 32 32h32c17.67 0 32-14.33 32-32V304h144c17.67 0 32-14.33 32-32v-32c0-17.67-14.33-32-32-32z\"><\/path><\/svg><\/span>\n\t\t\t\t\t\t\t\t<span class=\"elementor-accordion-icon-opened\"><svg class=\"e-font-icon-svg e-fas-minus\" viewBox=\"0 0 448 512\" xmlns=\"http:\/\/www.w3.org\/2000\/svg\"><path d=\"M416 208H32c-17.67 0-32 14.33-32 32v32c0 17.67 14.33 32 32 32h384c17.67 0 32-14.33 32-32v-32c0-17.67-14.33-32-32-32z\"><\/path><\/svg><\/span>\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/span>\n\t\t\t\t\t\t\t\t\t\t\t\t<a class=\"elementor-accordion-title\" tabindex=\"0\">When should I harvest a CHO transient culture?<\/a>\n\t\t\t\t\t<\/div>\n\t\t\t\t\t<div id=\"elementor-tab-content-4655\" class=\"elementor-tab-content elementor-clearfix\" data-tab=\"5\" role=\"region\" aria-labelledby=\"elementor-tab-title-4655\"><p>Harvest on Day 7, when viability falls to about 75%. With extra feeding, Celetrix-electroporated CHO cultures can run to Day 10\u201312.<\/p><\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t\t\t\t\t\t<script type=\"application\/ld+json\">{\"@context\":\"https:\\\/\\\/schema.org\",\"@type\":\"FAQPage\",\"mainEntity\":[{\"@type\":\"Question\",\"name\":\"Which cell lines work for large-scale transient expression by electroporation?\",\"acceptedAnswer\":{\"@type\":\"Answer\",\"text\":\"<p>Celetrix Model SLT electroporates CHO-K1, CHO-S, 293F and Vero cells, as well as stimulated T and NK cells. For unstimulated PBMCs, use Celetrix Model LE+ or EX+.<\\\/p>\"}},{\"@type\":\"Question\",\"name\":\"How can I scale up transient antibody production in CHO cells?\",\"acceptedAnswer\":{\"@type\":\"Answer\",\"text\":\"<p>Celetrix Model SLT electroporates 2\\u00d710<sup>9<\\\/sup> CHO cells in a single 10 ml tube for a 400 ml fed-batch culture (up to 1 L with transposons), reaching g\\\/L antibody titers in 7 days.<\\\/p>\"}},{\"@type\":\"Question\",\"name\":\"Do I need a special medium for CHO transient transfection?\",\"acceptedAnswer\":{\"@type\":\"Answer\",\"text\":\"<p>No. With Celetrix electroporation, medium choice is less critical than with chemical transfection. Choose a medium that supports high cell density and long culture.<\\\/p>\"}},{\"@type\":\"Question\",\"name\":\"How much plasmid DNA is needed for CHO transient transfection?\",\"acceptedAnswer\":{\"@type\":\"Answer\",\"text\":\"<p>For Celetrix electroporation, use 50\\u2013200 \\u00b5g\\\/ml plasmid DNA, typically 100 \\u00b5g\\\/ml, about 1 mg per 10 ml tube.<\\\/p>\"}},{\"@type\":\"Question\",\"name\":\"When should I harvest a CHO transient culture?\",\"acceptedAnswer\":{\"@type\":\"Answer\",\"text\":\"<p>Harvest on Day 7, when viability falls to about 75%. With extra feeding, Celetrix-electroporated CHO cultures can run to Day 10\\u201312.<\\\/p>\"}}]}<\/script>\n\t\t\t\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-d8c77d6 elementor-align-center elementor-widget elementor-widget-button\" data-id=\"d8c77d6\" data-element_type=\"widget\" data-widget_type=\"button.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<div class=\"elementor-button-wrapper\">\n\t\t\t\t\t<a class=\"elementor-button elementor-button-link elementor-size-sm\" href=\"https:\/\/www.celetrix.com\/ordering\/\">\n\t\t\t\t\t\t<span class=\"elementor-button-content-wrapper\">\n\t\t\t\t\t\t\t\t\t<span class=\"elementor-button-text\">Request a Quote<\/span>\n\t\t\t\t\t<\/span>\n\t\t\t\t\t<\/a>\n\t\t\t\t<\/div>\n\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t<div class=\"elementor-column elementor-col-50 elementor-top-column elementor-element elementor-element-23364442\" data-id=\"23364442\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-9578c36 elementor-nav-menu__align-start elementor-nav-menu--dropdown-none elementor-widget elementor-widget-nav-menu\" data-id=\"9578c36\" data-element_type=\"widget\" data-settings=\"{&quot;layout&quot;:&quot;vertical&quot;,&quot;submenu_icon&quot;:{&quot;value&quot;:&quot;&lt;svg aria-hidden=\\&quot;true\\&quot; 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