{"id":1127,"date":"2023-01-12T07:08:13","date_gmt":"2023-01-12T07:08:13","guid":{"rendered":"https:\/\/www.celetrix.com\/?page_id=1127"},"modified":"2023-01-17T09:58:26","modified_gmt":"2023-01-17T09:58:26","slug":"u937","status":"publish","type":"page","link":"https:\/\/www.celetrix.com\/zh\/recommend-parameters\/u937\/","title":{"rendered":"U937"},"content":{"rendered":"<div data-elementor-type=\"wp-page\" data-elementor-id=\"1127\" class=\"elementor elementor-1127\" data-elementor-post-type=\"page\">\n\t\t\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-53c5a98 elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"53c5a98\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-50 elementor-top-column elementor-element elementor-element-bdbb233\" data-id=\"bdbb233\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-e2689c9 elementor-widget elementor-widget-bdt-breadcrumbs\" data-id=\"e2689c9\" data-element_type=\"widget\" data-widget_type=\"bdt-breadcrumbs.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<div class='bdt-breadcrumbs-wrapper'><ul class=\"bdt-breadcrumb breadcrumb-align-left\"><li><a href=\"https:\/\/www.celetrix.com\/zh\/\">Electroporation by Celetrix<\/a><\/li>\n<li><span> \u9875\u9762<\/span><\/li>\n<\/ul><\/div>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-b7af868 elementor-widget elementor-widget-heading\" data-id=\"b7af868\" data-element_type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h2 class=\"elementor-heading-title elementor-size-default\">U937<\/h2>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-a7587bb elementor-widget elementor-widget-image\" data-id=\"a7587bb\" data-element_type=\"widget\" data-widget_type=\"image.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<img fetchpriority=\"high\" decoding=\"async\" width=\"476\" height=\"181\" src=\"https:\/\/www.celetrix.com\/wp-content\/uploads\/2023\/01\/U937.png\" class=\"attachment-large size-large wp-image-1131\"  srcset=\"https:\/\/www.celetrix.com\/wp-content\/uploads\/2023\/01\/U937.png 476w, https:\/\/www.celetrix.com\/wp-content\/uploads\/2023\/01\/U937-300x114.png 300w, https:\/\/www.celetrix.com\/wp-content\/uploads\/2023\/01\/U937-18x7.png 18w\" sizes=\"(max-width: 476px) 100vw, 476px\" title=\"U937\u63d2\u56fe\" alt=\"U937\u63d2\u56fe\" \/>\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-aa3a850 elementor-widget elementor-widget-text-editor\" data-id=\"aa3a850\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p>\u00a0 \u00a0 \u00a0 \u00a0The U937 cells are easy to transfect with the Celetrix electroporation system. Follow the suggested parameters and a high transfection efficiency would be achieved. Plasmids tend to cause cell death after electroporation in U937 cells. mRNA or protein (Cas9 RNP) does not cause cell death after electroporation. When using plasmids to generate a new engineered cell line, it is helpful to use a cell sorter to pick surviving cells from the transfected pool. The dying cells have a very distinct FSC\/SSC profile and it is easy to collect surviving cells from the transfected pool.\u00a0<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<section class=\"elementor-section elementor-inner-section elementor-element elementor-element-1edd85b elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"1edd85b\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-50 elementor-inner-column elementor-element elementor-element-17ef103\" data-id=\"17ef103\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-03d9938 elementor-widget elementor-widget-text-editor\" data-id=\"03d9938\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<h5 style=\"text-align: center;\"><strong style=\"color: inherit; font-family: inherit; font-size: 1.25rem;\">U937 in 20ul tubes<\/strong><\/h5><p>\u00a0 Cell Number\uff1a1-2*10^6 cells<\/p><p>\u00a0 Plasmid\uff1a2ug<\/p><p>\u00a0 E buffer\uff1aCeletrix buffer<\/p><p>\u00a0 Suggested parameter\uff1aCell Line Mode\uff0c500V-540V<\/p><p>\u00a0 Culture medium: 1640+10%FBS\u00a0 (antibiotics free)<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t<div class=\"elementor-column elementor-col-50 elementor-inner-column elementor-element elementor-element-e801f1c\" data-id=\"e801f1c\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-d21d504 elementor-widget elementor-widget-text-editor\" data-id=\"d21d504\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<h5 style=\"text-align: center;\"><strong style=\"color: inherit; font-family: inherit; font-size: 1.25rem;\">U937 in 100ul tubes<\/strong><\/h5><p>\u00a0 Cell Number\uff1a5-10*10^6 cells<\/p><p>\u00a0 Plasmid\uff1a10ug<\/p><p>\u00a0 E buffer\uff1aCeletrix buffer<\/p><p>\u00a0 Suggested parameter\uff1aCell Line Mode\uff0c500V-540V<\/p><p>\u00a0 Culture medium: 1640+10%FBS\u00a0 (antibiotics free)<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t<div class=\"elementor-column elementor-col-50 elementor-top-column elementor-element elementor-element-8db4f2c\" data-id=\"8db4f2c\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-d89b538 elementor-nav-menu__align-start elementor-nav-menu--dropdown-none elementor-widget elementor-widget-nav-menu\" data-id=\"d89b538\" data-element_type=\"widget\" data-settings=\"{&quot;layout&quot;:&quot;vertical&quot;,&quot;submenu_icon&quot;:{&quot;value&quot;:&quot;&lt;i class=\\&quot;fas fa-caret-down\\&quot; aria-hidden=\\&quot;true\\&quot;&gt;&lt;\\\/i&gt;&quot;,&quot;library&quot;:&quot;fa-solid&quot;}}\" data-widget_type=\"nav-menu.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t<nav aria-label=\"Menu\" class=\"elementor-nav-menu--main elementor-nav-menu__container elementor-nav-menu--layout-vertical e--pointer-background e--animation-fade\">\n\t\t\t\t<ul id=\"menu-1-d89b538\" class=\"elementor-nav-menu sm-vertical\"><li class=\"menu-item menu-item-type-post_type menu-item-object-page menu-item-105\"><a href=\"https:\/\/www.celetrix.com\/zh\/manuals-videos\/\" class=\"elementor-item\">Manuals &#038; Videos<\/a><\/li>\n<li class=\"menu-item menu-item-type-post_type menu-item-object-page menu-item-107\"><a href=\"https:\/\/www.celetrix.com\/zh\/recommend-parameters\/\" class=\"elementor-item\">Recommend Parameters<\/a><\/li>\n<li class=\"menu-item menu-item-type-post_type menu-item-object-page menu-item-106\"><a href=\"https:\/\/www.celetrix.com\/zh\/optimization-strategy\/\" class=\"elementor-item\">Optimization Strategy<\/a><\/li>\n<li class=\"menu-item menu-item-type-post_type menu-item-object-page menu-item-104\"><a href=\"https:\/\/www.celetrix.com\/zh\/experimental-overview\/\" class=\"elementor-item\">Experimental Overview<\/a><\/li>\n<li class=\"menu-item menu-item-type-post_type menu-item-object-page menu-item-108\"><a href=\"https:\/\/www.celetrix.com\/zh\/special-cells\/\" class=\"elementor-item\">Special Cells<\/a><\/li>\n<\/ul>\t\t\t<\/nav>\n\t\t\t\t\t\t<nav class=\"elementor-nav-menu--dropdown elementor-nav-menu__container\" aria-hidden=\"true\">\n\t\t\t\t<ul id=\"menu-2-d89b538\" class=\"elementor-nav-menu sm-vertical\"><li class=\"menu-item menu-item-type-post_type menu-item-object-page menu-item-105\"><a href=\"https:\/\/www.celetrix.com\/zh\/manuals-videos\/\" class=\"elementor-item\" tabindex=\"-1\">Manuals &#038; Videos<\/a><\/li>\n<li class=\"menu-item menu-item-type-post_type menu-item-object-page menu-item-107\"><a href=\"https:\/\/www.celetrix.com\/zh\/recommend-parameters\/\" class=\"elementor-item\" tabindex=\"-1\">Recommend Parameters<\/a><\/li>\n<li class=\"menu-item menu-item-type-post_type menu-item-object-page menu-item-106\"><a href=\"https:\/\/www.celetrix.com\/zh\/optimization-strategy\/\" class=\"elementor-item\" tabindex=\"-1\">Optimization Strategy<\/a><\/li>\n<li class=\"menu-item menu-item-type-post_type menu-item-object-page menu-item-104\"><a href=\"https:\/\/www.celetrix.com\/zh\/experimental-overview\/\" class=\"elementor-item\" tabindex=\"-1\">Experimental Overview<\/a><\/li>\n<li class=\"menu-item menu-item-type-post_type menu-item-object-page menu-item-108\"><a href=\"https:\/\/www.celetrix.com\/zh\/special-cells\/\" class=\"elementor-item\" tabindex=\"-1\">Special Cells<\/a><\/li>\n<\/ul>\t\t\t<\/nav>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<\/div>","protected":false},"excerpt":{"rendered":"<p>Electroporation by Celetrix Page U937 \u00a0 \u00a0 \u00a0 \u00a0The U937 cells are easy to transfect with the Celetrix electroporation system. Follow the suggested parameters and a high transfection efficiency would be achieved. Plasmids tend to cause cell death after electroporation in U937 cells. mRNA or protein (Cas9 RNP) does not cause cell death after electroporation. [&hellip;]<\/p>","protected":false},"author":1,"featured_media":0,"parent":51,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"elementor_header_footer","meta":{"om_disable_all_campaigns":false,"_uf_show_specific_survey":0,"_uf_disable_surveys":false,"footnotes":""},"class_list":["post-1127","page","type-page","status-publish","hentry"],"aioseo_notices":[],"_links":{"self":[{"href":"https:\/\/www.celetrix.com\/zh\/wp-json\/wp\/v2\/pages\/1127","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.celetrix.com\/zh\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/www.celetrix.com\/zh\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/www.celetrix.com\/zh\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.celetrix.com\/zh\/wp-json\/wp\/v2\/comments?post=1127"}],"version-history":[{"count":14,"href":"https:\/\/www.celetrix.com\/zh\/wp-json\/wp\/v2\/pages\/1127\/revisions"}],"predecessor-version":[{"id":1396,"href":"https:\/\/www.celetrix.com\/zh\/wp-json\/wp\/v2\/pages\/1127\/revisions\/1396"}],"up":[{"embeddable":true,"href":"https:\/\/www.celetrix.com\/zh\/wp-json\/wp\/v2\/pages\/51"}],"wp:attachment":[{"href":"https:\/\/www.celetrix.com\/zh\/wp-json\/wp\/v2\/media?parent=1127"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}