{"id":1108,"date":"2022-12-16T08:33:42","date_gmt":"2022-12-16T08:33:42","guid":{"rendered":"https:\/\/www.celetrix.com\/?page_id=1108"},"modified":"2026-10-26T05:00:37","modified_gmt":"2026-10-26T05:00:37","slug":"jurkat","status":"publish","type":"page","link":"https:\/\/www.celetrix.com\/zh\/recommend-parameters\/jurkat\/","title":{"rendered":"Jurkat"},"content":{"rendered":"\t\t<div data-elementor-type=\"wp-page\" data-elementor-id=\"1108\" class=\"elementor elementor-1108\" data-elementor-post-type=\"page\">\n\t\t\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-c8f909e elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"c8f909e\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-50 elementor-top-column elementor-element elementor-element-8319a4c\" data-id=\"8319a4c\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-c863017 elementor-widget elementor-widget-bdt-breadcrumbs\" data-id=\"c863017\" data-element_type=\"widget\" data-widget_type=\"bdt-breadcrumbs.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<div class='bdt-breadcrumbs-wrapper'><ul class=\"bdt-breadcrumb breadcrumb-align-left  \"><li><a href=\"https:\/\/www.celetrix.com\/zh\/\"><span class=\"bdt-breadcrumbs-home-icon\"> <i aria-hidden=\"true\" class=\"fas fa-home\"><\/i> <\/span>Electroporation by Celetrix<\/a><\/li>\n<li><span> \u9875\u9762<\/span><\/li>\n<\/ul><\/div>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-dfb8aa5 elementor-widget elementor-widget-heading\" data-id=\"dfb8aa5\" data-element_type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h1 class=\"elementor-heading-title elementor-size-default\">Jurkat Electroporation Protocol<\/h1>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-ddabb1f elementor-widget elementor-widget-text-editor\" data-id=\"ddabb1f\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p>Jurkat cells are hard to transfect with lipid reagents. With Celetrix electroporation, Jurkat cells reach &gt;90% transfection efficiency and keep growing fast afterwards. Below are our protocol and recommended parameters.<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-59233f4 elementor-widget elementor-widget-heading\" data-id=\"59233f4\" data-element_type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h2 class=\"elementor-heading-title elementor-size-default\">Jurkat Transfection Results<\/h2>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<section class=\"elementor-section elementor-inner-section elementor-element elementor-element-70c712d elementor-section-content-middle elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"70c712d\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-50 elementor-inner-column elementor-element elementor-element-b3c50d2\" data-id=\"b3c50d2\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-6f59b20 elementor-widget elementor-widget-image\" data-id=\"6f59b20\" data-element_type=\"widget\" data-widget_type=\"image.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t\t\t\t<figure class=\"wp-caption\">\n\t\t\t\t\t\t\t\t\t\t<img fetchpriority=\"high\" decoding=\"async\" width=\"605\" height=\"220\" src=\"https:\/\/www.celetrix.com\/wp-content\/uploads\/celetrix-jurkat-gfp-transfection-microscopy.jpg\" class=\"attachment-full size-full wp-image-2030\" alt=\"Side-by-side brightfield and fluorescence microscopy images demonstrating high GFP expression in Jurkat cells using Celetrix electroporation.\" srcset=\"https:\/\/www.celetrix.com\/wp-content\/uploads\/celetrix-jurkat-gfp-transfection-microscopy.jpg 605w, https:\/\/www.celetrix.com\/wp-content\/uploads\/celetrix-jurkat-gfp-transfection-microscopy-300x109.jpg 300w, https:\/\/www.celetrix.com\/wp-content\/uploads\/celetrix-jurkat-gfp-transfection-microscopy-18x7.jpg 18w\" sizes=\"(max-width: 605px) 100vw, 605px\" \/>\t\t\t\t\t\t\t\t\t\t\t<figcaption class=\"widget-image-caption wp-caption-text\">18 h: GFP expression in Jurkat cells<\/figcaption>\n\t\t\t\t\t\t\t\t\t\t<\/figure>\n\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t<div class=\"elementor-column elementor-col-50 elementor-inner-column elementor-element elementor-element-468c8a5\" data-id=\"468c8a5\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-cbfa5e4 elementor-widget elementor-widget-image\" data-id=\"cbfa5e4\" data-element_type=\"widget\" data-widget_type=\"image.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t\t\t\t<figure class=\"wp-caption\">\n\t\t\t\t\t\t\t\t\t\t<img decoding=\"async\" width=\"1254\" height=\"691\" src=\"https:\/\/www.celetrix.com\/wp-content\/uploads\/celetrix-electroporation-jurkat-gfp-18h-flow.png\" class=\"attachment-full size-full wp-image-2530\" alt=\"Celetrix electroporation of Jurkat cells: flow cytometry shows 90.27% GFP+ cells 18 h after transfection\" srcset=\"https:\/\/www.celetrix.com\/wp-content\/uploads\/celetrix-electroporation-jurkat-gfp-18h-flow.png 1254w, https:\/\/www.celetrix.com\/wp-content\/uploads\/celetrix-electroporation-jurkat-gfp-18h-flow-300x165.png 300w, https:\/\/www.celetrix.com\/wp-content\/uploads\/celetrix-electroporation-jurkat-gfp-18h-flow-1024x564.png 1024w, https:\/\/www.celetrix.com\/wp-content\/uploads\/celetrix-electroporation-jurkat-gfp-18h-flow-768x423.png 768w, https:\/\/www.celetrix.com\/wp-content\/uploads\/celetrix-electroporation-jurkat-gfp-18h-flow-18x10.png 18w\" sizes=\"(max-width: 1254px) 100vw, 1254px\" \/>\t\t\t\t\t\t\t\t\t\t\t<figcaption class=\"widget-image-caption wp-caption-text\">Flow cytometry: 90.27% GFP+ Jurkat cells<\/figcaption>\n\t\t\t\t\t\t\t\t\t\t<\/figure>\n\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<div class=\"elementor-element elementor-element-b6e6964 elementor-widget elementor-widget-heading\" data-id=\"b6e6964\" data-element_type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h2 class=\"elementor-heading-title elementor-size-default\">Recommended Parameters for Jurkat<\/h2>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-372535f elementor-widget elementor-widget-text-editor\" data-id=\"372535f\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<table class=\"cx-scale-table\" style=\"width:100%;border-collapse:collapse;table-layout:fixed;font-family:Montserrat,sans-serif;font-size:16px;\"><tbody><tr><td style=\"text-align:left;vertical-align:middle;padding:14px 16px;border-bottom:1px solid #dee2e6;overflow-wrap:anywhere;width:26%;font-weight:600;\">20 \u00b5l tube<\/td><td style=\"text-align:left;vertical-align:middle;padding:14px 16px;border-bottom:1px solid #dee2e6;overflow-wrap:anywhere;\">1\u20132\u00d710<sup>6<\/sup> cells, 2 \u00b5g plasmid, Cell Line mode 500\u2013540 V<\/td><\/tr><tr><td style=\"text-align:left;vertical-align:middle;padding:14px 16px;border-bottom:1px solid #dee2e6;overflow-wrap:anywhere;width:26%;font-weight:600;\">100 \u00b5l tube<\/td><td style=\"text-align:left;vertical-align:middle;padding:14px 16px;border-bottom:1px solid #dee2e6;overflow-wrap:anywhere;\">5\u201310\u00d710<sup>6<\/sup> cells, 10 \u00b5g plasmid, Cell Line mode 500\u2013540 V<\/td><\/tr><tr><td style=\"text-align:left;vertical-align:middle;padding:14px 16px;border-bottom:1px solid #dee2e6;overflow-wrap:anywhere;width:26%;font-weight:600;\">200 \u00b5l \/ 1 ml tube<\/td><td style=\"text-align:left;vertical-align:middle;padding:14px 16px;border-bottom:1px solid #dee2e6;overflow-wrap:anywhere;\">Cell Line mode 1200\u20131350 V<\/td><\/tr><tr><td style=\"text-align:left;vertical-align:middle;padding:14px 16px;border-bottom:1px solid #dee2e6;overflow-wrap:anywhere;width:26%;font-weight:600;\">Buffer<\/td><td style=\"text-align:left;vertical-align:middle;padding:14px 16px;border-bottom:1px solid #dee2e6;overflow-wrap:anywhere;\">Celetrix electroporation buffer<\/td><\/tr><tr><td style=\"text-align:left;vertical-align:middle;padding:14px 16px;border-bottom:1px solid #dee2e6;overflow-wrap:anywhere;width:26%;font-weight:600;\">Recovery medium<\/td><td style=\"text-align:left;vertical-align:middle;padding:14px 16px;border-bottom:1px solid #dee2e6;overflow-wrap:anywhere;\">RPMI 1640 + 10% FBS, antibiotic-free<\/td><\/tr><\/tbody><\/table>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-c900953 elementor-widget elementor-widget-heading\" data-id=\"c900953\" data-element_type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h2 class=\"elementor-heading-title elementor-size-default\">Step-by-Step Jurkat Electroporation Protocol<\/h2>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-894bfb9 elementor-widget elementor-widget-text-editor\" data-id=\"894bfb9\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<ol><li>Culture Jurkat cells to 50\u201370% of their maximum density.<\/li><li>Spin down cells at 1000\u20131200 rpm for 3 min.<\/li><li>Resuspend in Celetrix electroporation buffer (1\u20132\u00d710<sup>6<\/sup> cells per 20 \u00b5l).<\/li><li>Add plasmid DNA, fill the tube until a small dome forms, and cap it.<\/li><li>Electroporate right away in Cell Line mode at 500\u2013540 V.<\/li><li>Transfer cells to warm, antibiotic-free RPMI 1640 + 10% FBS. GFP is visible within 1\u20133 h and stronger overnight.<\/li><\/ol>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-18dad38 elementor-widget elementor-widget-heading\" data-id=\"18dad38\" data-element_type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h2 class=\"elementor-heading-title elementor-size-default\">Jurkat Knockout with Cas9 RNP<\/h2>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-32c8325 elementor-widget elementor-widget-image\" data-id=\"32c8325\" data-element_type=\"widget\" data-widget_type=\"image.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t\t\t\t<figure class=\"wp-caption\">\n\t\t\t\t\t\t\t\t\t\t<img decoding=\"async\" width=\"1278\" height=\"691\" src=\"https:\/\/www.celetrix.com\/wp-content\/uploads\/celetrix-electroporation-jurkat-cd3e-ko-flow-cytometry.png\" class=\"attachment-full size-full wp-image-2535\" alt=\"Celetrix electroporation of Cas9 RNP knocks out CD3e in Jurkat cells: flow cytometry shows 92% CD3e-negative cells\" srcset=\"https:\/\/www.celetrix.com\/wp-content\/uploads\/celetrix-electroporation-jurkat-cd3e-ko-flow-cytometry.png 1278w, https:\/\/www.celetrix.com\/wp-content\/uploads\/celetrix-electroporation-jurkat-cd3e-ko-flow-cytometry-300x162.png 300w, https:\/\/www.celetrix.com\/wp-content\/uploads\/celetrix-electroporation-jurkat-cd3e-ko-flow-cytometry-1024x554.png 1024w, https:\/\/www.celetrix.com\/wp-content\/uploads\/celetrix-electroporation-jurkat-cd3e-ko-flow-cytometry-768x415.png 768w, https:\/\/www.celetrix.com\/wp-content\/uploads\/celetrix-electroporation-jurkat-cd3e-ko-flow-cytometry-18x10.png 18w\" sizes=\"(max-width: 1278px) 100vw, 1278px\" \/>\t\t\t\t\t\t\t\t\t\t\t<figcaption class=\"widget-image-caption wp-caption-text\">Cas9 RNP knockout: CD3e+ Jurkat cells drop from 93.39% to 7.95%.<\/figcaption>\n\t\t\t\t\t\t\t\t\t\t<\/figure>\n\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-8034047 elementor-widget elementor-widget-text-editor\" data-id=\"8034047\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p style=\"margin:0 0 10px;\">For a 20 \u00b5l Celetrix tube:<\/p><table class=\"cx-scale-table\" style=\"width:100%;border-collapse:collapse;table-layout:fixed;font-family:Montserrat,sans-serif;font-size:16px;\"><tbody><tr><td style=\"text-align:left;vertical-align:middle;padding:14px 16px;border-bottom:1px solid #dee2e6;overflow-wrap:anywhere;width:26%;font-weight:600;\">Cells<\/td><td style=\"text-align:left;vertical-align:middle;padding:14px 16px;border-bottom:1px solid #dee2e6;overflow-wrap:anywhere;\">1\u20132\u00d710<sup>6<\/sup> cells, pelleted<\/td><\/tr><tr><td style=\"text-align:left;vertical-align:middle;padding:14px 16px;border-bottom:1px solid #dee2e6;overflow-wrap:anywhere;width:26%;font-weight:600;\">Cas9 protein<\/td><td style=\"text-align:left;vertical-align:middle;padding:14px 16px;border-bottom:1px solid #dee2e6;overflow-wrap:anywhere;\">5 \u00b5g<\/td><\/tr><tr><td style=\"text-align:left;vertical-align:middle;padding:14px 16px;border-bottom:1px solid #dee2e6;overflow-wrap:anywhere;width:26%;font-weight:600;\">sgRNA<\/td><td style=\"text-align:left;vertical-align:middle;padding:14px 16px;border-bottom:1px solid #dee2e6;overflow-wrap:anywhere;\">50\u2013100 pmol (for multiple sgRNAs, add Cas9 protein last)<\/td><\/tr><tr><td style=\"text-align:left;vertical-align:middle;padding:14px 16px;border-bottom:1px solid #dee2e6;overflow-wrap:anywhere;width:26%;font-weight:600;\">RNP assembly<\/td><td style=\"text-align:left;vertical-align:middle;padding:14px 16px;border-bottom:1px solid #dee2e6;overflow-wrap:anywhere;\">Mix Cas9 and sgRNA in 22 \u00b5l Celetrix electroporation buffer; incubate 10 min at room temperature<\/td><\/tr><tr><td style=\"text-align:left;vertical-align:middle;padding:14px 16px;border-bottom:1px solid #dee2e6;overflow-wrap:anywhere;width:26%;font-weight:600;\">Electroporation<\/td><td style=\"text-align:left;vertical-align:middle;padding:14px 16px;border-bottom:1px solid #dee2e6;overflow-wrap:anywhere;\">Add the RNP mix to the cell pellet, mix well and electroporate (about 25 \u00b5l total)<\/td><\/tr><\/tbody><\/table><div style=\"background:#F5F8FB;border-left:4px solid #0078B5;padding:14px 18px;margin-top:16px;\"><p style=\"margin:0 0 6px;font-weight:700;color:#0078B5;\">Tips for Jurkat<\/p><ul style=\"margin:0;padding-left:18px;\"><li>Cas9 RNP usually needs a slightly higher voltage than plasmid.<\/li><li>About 10\u201320% cell death is normal; if there is a lot of cell death, lower the voltage by 20\u201330 V.<\/li><li>Keep cells as single cells and electroporate each tube right after loading.<\/li><li>More tips in our <a href=\"\/optimization-strategy\/\">electroporation optimization guide<\/a>.<\/li><\/ul><\/div>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-a54b562 elementor-widget elementor-widget-heading\" data-id=\"a54b562\" data-element_type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h2 class=\"elementor-heading-title elementor-size-default\">Jurkat Electroporation FAQs<\/h2>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-df63e57 elementor-widget elementor-widget-accordion\" data-id=\"df63e57\" data-element_type=\"widget\" data-widget_type=\"accordion.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t<div class=\"elementor-accordion\">\n\t\t\t\t\t\t\t<div class=\"elementor-accordion-item\">\n\t\t\t\t\t<div id=\"elementor-tab-title-2341\" class=\"elementor-tab-title\" data-tab=\"1\" role=\"button\" aria-controls=\"elementor-tab-content-2341\" aria-expanded=\"false\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t<span class=\"elementor-accordion-icon elementor-accordion-icon-left\" aria-hidden=\"true\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<span class=\"elementor-accordion-icon-closed\"><svg class=\"e-font-icon-svg e-fas-plus\" viewBox=\"0 0 448 512\" xmlns=\"http:\/\/www.w3.org\/2000\/svg\"><path d=\"M416 208H272V64c0-17.67-14.33-32-32-32h-32c-17.67 0-32 14.33-32 32v144H32c-17.67 0-32 14.33-32 32v32c0 17.67 14.33 32 32 32h144v144c0 17.67 14.33 32 32 32h32c17.67 0 32-14.33 32-32V304h144c17.67 0 32-14.33 32-32v-32c0-17.67-14.33-32-32-32z\"><\/path><\/svg><\/span>\n\t\t\t\t\t\t\t\t<span class=\"elementor-accordion-icon-opened\"><svg class=\"e-font-icon-svg e-fas-minus\" viewBox=\"0 0 448 512\" xmlns=\"http:\/\/www.w3.org\/2000\/svg\"><path d=\"M416 208H32c-17.67 0-32 14.33-32 32v32c0 17.67 14.33 32 32 32h384c17.67 0 32-14.33 32-32v-32c0-17.67-14.33-32-32-32z\"><\/path><\/svg><\/span>\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/span>\n\t\t\t\t\t\t\t\t\t\t\t\t<a class=\"elementor-accordion-title\" tabindex=\"0\">Why does electroporation give better cell viability than transfection reagents?<\/a>\n\t\t\t\t\t<\/div>\n\t\t\t\t\t<div id=\"elementor-tab-content-2341\" class=\"elementor-tab-content elementor-clearfix\" data-tab=\"1\" role=\"region\" aria-labelledby=\"elementor-tab-title-2341\"><p>Electroporation acts at the cell membrane, so its small toxicity stays at the membrane and has little effect inside the cell. Lipid and other chemical transfection reagents affect not only the membrane but also components inside the cell. That is why Celetrix electroporation keeps cell toxicity low and Jurkat cells healthier than transfection reagents. Learn how the voltage falls on the cell membrane in our <a href=\"https:\/\/www.celetrix.com\/electroporation-mechanism\/\">electroporation mechanism<\/a> page.<\/p><\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t\t\t<div class=\"elementor-accordion-item\">\n\t\t\t\t\t<div id=\"elementor-tab-title-2342\" class=\"elementor-tab-title\" data-tab=\"2\" role=\"button\" aria-controls=\"elementor-tab-content-2342\" aria-expanded=\"false\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t<span class=\"elementor-accordion-icon elementor-accordion-icon-left\" aria-hidden=\"true\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<span class=\"elementor-accordion-icon-closed\"><svg class=\"e-font-icon-svg e-fas-plus\" viewBox=\"0 0 448 512\" xmlns=\"http:\/\/www.w3.org\/2000\/svg\"><path d=\"M416 208H272V64c0-17.67-14.33-32-32-32h-32c-17.67 0-32 14.33-32 32v144H32c-17.67 0-32 14.33-32 32v32c0 17.67 14.33 32 32 32h144v144c0 17.67 14.33 32 32 32h32c17.67 0 32-14.33 32-32V304h144c17.67 0 32-14.33 32-32v-32c0-17.67-14.33-32-32-32z\"><\/path><\/svg><\/span>\n\t\t\t\t\t\t\t\t<span class=\"elementor-accordion-icon-opened\"><svg class=\"e-font-icon-svg e-fas-minus\" viewBox=\"0 0 448 512\" xmlns=\"http:\/\/www.w3.org\/2000\/svg\"><path d=\"M416 208H32c-17.67 0-32 14.33-32 32v32c0 17.67 14.33 32 32 32h384c17.67 0 32-14.33 32-32v-32c0-17.67-14.33-32-32-32z\"><\/path><\/svg><\/span>\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/span>\n\t\t\t\t\t\t\t\t\t\t\t\t<a class=\"elementor-accordion-title\" tabindex=\"0\">What voltage should I use to electroporate Jurkat cells?<\/a>\n\t\t\t\t\t<\/div>\n\t\t\t\t\t<div id=\"elementor-tab-content-2342\" class=\"elementor-tab-content elementor-clearfix\" data-tab=\"2\" role=\"region\" aria-labelledby=\"elementor-tab-title-2342\"><p>On Celetrix electroporators, use Cell Line mode at 500\u2013540 V for 20 \u00b5l and 100 \u00b5l tubes, and 1200\u20131350 V for 200 \u00b5l and 1 ml tubes. See all Celetrix <a href=\"\/recommend-parameters\/\">recommended parameters<\/a>.<\/p><\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t\t\t<div class=\"elementor-accordion-item\">\n\t\t\t\t\t<div id=\"elementor-tab-title-2343\" class=\"elementor-tab-title\" data-tab=\"3\" role=\"button\" aria-controls=\"elementor-tab-content-2343\" aria-expanded=\"false\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t<span class=\"elementor-accordion-icon elementor-accordion-icon-left\" aria-hidden=\"true\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<span class=\"elementor-accordion-icon-closed\"><svg class=\"e-font-icon-svg e-fas-plus\" viewBox=\"0 0 448 512\" xmlns=\"http:\/\/www.w3.org\/2000\/svg\"><path d=\"M416 208H272V64c0-17.67-14.33-32-32-32h-32c-17.67 0-32 14.33-32 32v144H32c-17.67 0-32 14.33-32 32v32c0 17.67 14.33 32 32 32h144v144c0 17.67 14.33 32 32 32h32c17.67 0 32-14.33 32-32V304h144c17.67 0 32-14.33 32-32v-32c0-17.67-14.33-32-32-32z\"><\/path><\/svg><\/span>\n\t\t\t\t\t\t\t\t<span class=\"elementor-accordion-icon-opened\"><svg class=\"e-font-icon-svg e-fas-minus\" viewBox=\"0 0 448 512\" xmlns=\"http:\/\/www.w3.org\/2000\/svg\"><path d=\"M416 208H32c-17.67 0-32 14.33-32 32v32c0 17.67 14.33 32 32 32h384c17.67 0 32-14.33 32-32v-32c0-17.67-14.33-32-32-32z\"><\/path><\/svg><\/span>\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/span>\n\t\t\t\t\t\t\t\t\t\t\t\t<a class=\"elementor-accordion-title\" tabindex=\"0\">Why does Celetrix give a voltage range instead of a single voltage?<\/a>\n\t\t\t\t\t<\/div>\n\t\t\t\t\t<div id=\"elementor-tab-content-2343\" class=\"elementor-tab-content elementor-clearfix\" data-tab=\"3\" role=\"region\" aria-labelledby=\"elementor-tab-title-2343\"><p>The best voltage depends on your sample. Higher cell density needs a slightly higher voltage. With larger plasmids, cells tolerate high voltage less well, so lower the voltage slightly. That is why Celetrix gives a voltage range instead of one fixed voltage: choose a value within the range to match your cell density and plasmid size.<\/p><\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t\t\t<div class=\"elementor-accordion-item\">\n\t\t\t\t\t<div id=\"elementor-tab-title-2344\" class=\"elementor-tab-title\" data-tab=\"4\" role=\"button\" aria-controls=\"elementor-tab-content-2344\" aria-expanded=\"false\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t<span class=\"elementor-accordion-icon elementor-accordion-icon-left\" aria-hidden=\"true\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<span class=\"elementor-accordion-icon-closed\"><svg class=\"e-font-icon-svg e-fas-plus\" viewBox=\"0 0 448 512\" xmlns=\"http:\/\/www.w3.org\/2000\/svg\"><path d=\"M416 208H272V64c0-17.67-14.33-32-32-32h-32c-17.67 0-32 14.33-32 32v144H32c-17.67 0-32 14.33-32 32v32c0 17.67 14.33 32 32 32h144v144c0 17.67 14.33 32 32 32h32c17.67 0 32-14.33 32-32V304h144c17.67 0 32-14.33 32-32v-32c0-17.67-14.33-32-32-32z\"><\/path><\/svg><\/span>\n\t\t\t\t\t\t\t\t<span class=\"elementor-accordion-icon-opened\"><svg class=\"e-font-icon-svg e-fas-minus\" viewBox=\"0 0 448 512\" xmlns=\"http:\/\/www.w3.org\/2000\/svg\"><path d=\"M416 208H32c-17.67 0-32 14.33-32 32v32c0 17.67 14.33 32 32 32h384c17.67 0 32-14.33 32-32v-32c0-17.67-14.33-32-32-32z\"><\/path><\/svg><\/span>\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/span>\n\t\t\t\t\t\t\t\t\t\t\t\t<a class=\"elementor-accordion-title\" tabindex=\"0\">How many Jurkat cells do I need for electroporation?<\/a>\n\t\t\t\t\t<\/div>\n\t\t\t\t\t<div id=\"elementor-tab-content-2344\" class=\"elementor-tab-content elementor-clearfix\" data-tab=\"4\" role=\"region\" aria-labelledby=\"elementor-tab-title-2344\"><p>For Celetrix electroporation, use 1\u20132\u00d710<sup>6<\/sup> Jurkat cells per 20 \u00b5l tube or 5\u201310\u00d710<sup>6<\/sup> cells per 100 \u00b5l tube.<\/p><\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t\t\t<div class=\"elementor-accordion-item\">\n\t\t\t\t\t<div id=\"elementor-tab-title-2345\" class=\"elementor-tab-title\" data-tab=\"5\" role=\"button\" aria-controls=\"elementor-tab-content-2345\" aria-expanded=\"false\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t<span class=\"elementor-accordion-icon elementor-accordion-icon-left\" aria-hidden=\"true\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<span class=\"elementor-accordion-icon-closed\"><svg class=\"e-font-icon-svg e-fas-plus\" viewBox=\"0 0 448 512\" xmlns=\"http:\/\/www.w3.org\/2000\/svg\"><path d=\"M416 208H272V64c0-17.67-14.33-32-32-32h-32c-17.67 0-32 14.33-32 32v144H32c-17.67 0-32 14.33-32 32v32c0 17.67 14.33 32 32 32h144v144c0 17.67 14.33 32 32 32h32c17.67 0 32-14.33 32-32V304h144c17.67 0 32-14.33 32-32v-32c0-17.67-14.33-32-32-32z\"><\/path><\/svg><\/span>\n\t\t\t\t\t\t\t\t<span class=\"elementor-accordion-icon-opened\"><svg class=\"e-font-icon-svg e-fas-minus\" viewBox=\"0 0 448 512\" xmlns=\"http:\/\/www.w3.org\/2000\/svg\"><path d=\"M416 208H32c-17.67 0-32 14.33-32 32v32c0 17.67 14.33 32 32 32h384c17.67 0 32-14.33 32-32v-32c0-17.67-14.33-32-32-32z\"><\/path><\/svg><\/span>\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/span>\n\t\t\t\t\t\t\t\t\t\t\t\t<a class=\"elementor-accordion-title\" tabindex=\"0\">Can I knock out genes in Jurkat cells with Cas9 RNP?<\/a>\n\t\t\t\t\t<\/div>\n\t\t\t\t\t<div id=\"elementor-tab-content-2345\" class=\"elementor-tab-content elementor-clearfix\" data-tab=\"5\" role=\"region\" aria-labelledby=\"elementor-tab-title-2345\"><p>Yes. Celetrix recommends Cas9 RNP for Jurkat gene editing: 5 \u00b5g Cas9 protein with 50\u2013100 pmol sgRNA per 20 \u00b5l tube. RNP avoids the toxicity of Cas9 plasmids.<\/p><\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t\t\t<div class=\"elementor-accordion-item\">\n\t\t\t\t\t<div id=\"elementor-tab-title-2346\" class=\"elementor-tab-title\" data-tab=\"6\" role=\"button\" aria-controls=\"elementor-tab-content-2346\" aria-expanded=\"false\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t<span class=\"elementor-accordion-icon elementor-accordion-icon-left\" aria-hidden=\"true\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<span class=\"elementor-accordion-icon-closed\"><svg class=\"e-font-icon-svg e-fas-plus\" viewBox=\"0 0 448 512\" xmlns=\"http:\/\/www.w3.org\/2000\/svg\"><path d=\"M416 208H272V64c0-17.67-14.33-32-32-32h-32c-17.67 0-32 14.33-32 32v144H32c-17.67 0-32 14.33-32 32v32c0 17.67 14.33 32 32 32h144v144c0 17.67 14.33 32 32 32h32c17.67 0 32-14.33 32-32V304h144c17.67 0 32-14.33 32-32v-32c0-17.67-14.33-32-32-32z\"><\/path><\/svg><\/span>\n\t\t\t\t\t\t\t\t<span class=\"elementor-accordion-icon-opened\"><svg class=\"e-font-icon-svg e-fas-minus\" viewBox=\"0 0 448 512\" xmlns=\"http:\/\/www.w3.org\/2000\/svg\"><path d=\"M416 208H32c-17.67 0-32 14.33-32 32v32c0 17.67 14.33 32 32 32h384c17.67 0 32-14.33 32-32v-32c0-17.67-14.33-32-32-32z\"><\/path><\/svg><\/span>\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/span>\n\t\t\t\t\t\t\t\t\t\t\t\t<a class=\"elementor-accordion-title\" tabindex=\"0\">How do I keep Jurkat cells healthy after electroporation?<\/a>\n\t\t\t\t\t<\/div>\n\t\t\t\t\t<div id=\"elementor-tab-content-2346\" class=\"elementor-tab-content elementor-clearfix\" data-tab=\"6\" role=\"region\" aria-labelledby=\"elementor-tab-title-2346\"><p>Move cells into warm, antibiotic-free RPMI 1640 + 10% FBS right after the pulse. With Celetrix electroporation, about 10\u201320% cell death is normal; if there is a lot of cell death, lower the voltage by 20\u201330 V.<\/p><\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t\t\t\t\t\t<script type=\"application\/ld+json\">{\"@context\":\"https:\\\/\\\/schema.org\",\"@type\":\"FAQPage\",\"mainEntity\":[{\"@type\":\"Question\",\"name\":\"Why does electroporation give better cell viability than transfection reagents?\",\"acceptedAnswer\":{\"@type\":\"Answer\",\"text\":\"<p>Electroporation acts at the cell membrane, so its small toxicity stays at the membrane and has little effect inside the cell. Lipid and other chemical transfection reagents affect not only the membrane but also components inside the cell. That is why Celetrix electroporation keeps cell toxicity low and Jurkat cells healthier than transfection reagents. Learn how the voltage falls on the cell membrane in our <a href=\\\"https:\\\/\\\/www.celetrix.com\\\/electroporation-mechanism\\\/\\\">electroporation mechanism<\\\/a> page.<\\\/p>\"}},{\"@type\":\"Question\",\"name\":\"What voltage should I use to electroporate Jurkat cells?\",\"acceptedAnswer\":{\"@type\":\"Answer\",\"text\":\"<p>On Celetrix electroporators, use Cell Line mode at 500\\u2013540 V for 20 \\u00b5l and 100 \\u00b5l tubes, and 1200\\u20131350 V for 200 \\u00b5l and 1 ml tubes. See all Celetrix <a href=\\\"\\\/recommend-parameters\\\/\\\">recommended parameters<\\\/a>.<\\\/p>\"}},{\"@type\":\"Question\",\"name\":\"Why does Celetrix give a voltage range instead of a single voltage?\",\"acceptedAnswer\":{\"@type\":\"Answer\",\"text\":\"<p>The best voltage depends on your sample. Higher cell density needs a slightly higher voltage. With larger plasmids, cells tolerate high voltage less well, so lower the voltage slightly. That is why Celetrix gives a voltage range instead of one fixed voltage: choose a value within the range to match your cell density and plasmid size.<\\\/p>\"}},{\"@type\":\"Question\",\"name\":\"How many Jurkat cells do I need for electroporation?\",\"acceptedAnswer\":{\"@type\":\"Answer\",\"text\":\"<p>For Celetrix electroporation, use 1\\u20132\\u00d710<sup>6<\\\/sup> Jurkat cells per 20 \\u00b5l tube or 5\\u201310\\u00d710<sup>6<\\\/sup> cells per 100 \\u00b5l tube.<\\\/p>\"}},{\"@type\":\"Question\",\"name\":\"Can I knock out genes in Jurkat cells with Cas9 RNP?\",\"acceptedAnswer\":{\"@type\":\"Answer\",\"text\":\"<p>Yes. Celetrix recommends Cas9 RNP for Jurkat gene editing: 5 \\u00b5g Cas9 protein with 50\\u2013100 pmol sgRNA per 20 \\u00b5l tube. RNP avoids the toxicity of Cas9 plasmids.<\\\/p>\"}},{\"@type\":\"Question\",\"name\":\"How do I keep Jurkat cells healthy after electroporation?\",\"acceptedAnswer\":{\"@type\":\"Answer\",\"text\":\"<p>Move cells into warm, antibiotic-free RPMI 1640 + 10% FBS right after the pulse. With Celetrix electroporation, about 10\\u201320% cell death is normal; if there is a lot of cell death, lower the voltage by 20\\u201330 V.<\\\/p>\"}}]}<\/script>\n\t\t\t\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-61451ca elementor-align-center elementor-widget elementor-widget-button\" data-id=\"61451ca\" data-element_type=\"widget\" data-widget_type=\"button.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<div class=\"elementor-button-wrapper\">\n\t\t\t\t\t<a class=\"elementor-button elementor-button-link elementor-size-sm\" href=\"https:\/\/www.celetrix.com\/ordering\/\">\n\t\t\t\t\t\t<span class=\"elementor-button-content-wrapper\">\n\t\t\t\t\t\t\t\t\t<span class=\"elementor-button-text\">Request a Quote<\/span>\n\t\t\t\t\t<\/span>\n\t\t\t\t\t<\/a>\n\t\t\t\t<\/div>\n\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t<div class=\"elementor-column elementor-col-50 elementor-top-column elementor-element elementor-element-400692e\" data-id=\"400692e\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-8a0fca2 elementor-nav-menu__align-start elementor-nav-menu--dropdown-none elementor-widget elementor-widget-nav-menu\" data-id=\"8a0fca2\" data-element_type=\"widget\" data-settings=\"{&quot;layout&quot;:&quot;vertical&quot;,&quot;submenu_icon&quot;:{&quot;value&quot;:&quot;&lt;svg aria-hidden=\\&quot;true\\&quot; class=\\&quot;e-font-icon-svg e-fas-caret-down\\&quot; viewBox=\\&quot;0 0 320 512\\&quot; xmlns=\\&quot;http:\\\/\\\/www.w3.org\\\/2000\\\/svg\\&quot;&gt;&lt;path d=\\&quot;M31.3 192h257.3c17.8 0 26.7 21.5 14.1 34.1L174.1 354.8c-7.8 7.8-20.5 7.8-28.3 0L17.2 226.1C4.6 213.5 13.5 192 31.3 192z\\&quot;&gt;&lt;\\\/path&gt;&lt;\\\/svg&gt;&quot;,&quot;library&quot;:&quot;fa-solid&quot;}}\" data-widget_type=\"nav-menu.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t<nav aria-label=\"Menu\" class=\"elementor-nav-menu--main elementor-nav-menu__container elementor-nav-menu--layout-vertical e--pointer-background e--animation-fade\">\n\t\t\t\t<ul id=\"menu-1-8a0fca2\" class=\"elementor-nav-menu sm-vertical\"><li class=\"menu-item menu-item-type-post_type menu-item-object-page menu-item-105\"><a href=\"https:\/\/www.celetrix.com\/zh\/manuals-videos\/\" class=\"elementor-item\">Manuals &#038; Videos<\/a><\/li>\n<li class=\"menu-item menu-item-type-post_type menu-item-object-page menu-item-107\"><a href=\"https:\/\/www.celetrix.com\/zh\/recommend-parameters\/\" class=\"elementor-item\">Recommended Parameters<\/a><\/li>\n<li class=\"menu-item menu-item-type-post_type menu-item-object-page menu-item-106\"><a href=\"https:\/\/www.celetrix.com\/zh\/optimization-strategy\/\" class=\"elementor-item\">Optimization Strategy<\/a><\/li>\n<li class=\"menu-item menu-item-type-post_type menu-item-object-page menu-item-104\"><a href=\"https:\/\/www.celetrix.com\/zh\/experimental-overview\/\" class=\"elementor-item\">Experimental Overview<\/a><\/li>\n<li class=\"menu-item menu-item-type-post_type menu-item-object-page menu-item-108\"><a href=\"https:\/\/www.celetrix.com\/zh\/special-cells\/\" class=\"elementor-item\">Special Cells<\/a><\/li>\n<\/ul>\t\t\t<\/nav>\n\t\t\t\t\t\t<nav class=\"elementor-nav-menu--dropdown elementor-nav-menu__container\" aria-hidden=\"true\">\n\t\t\t\t<ul id=\"menu-2-8a0fca2\" class=\"elementor-nav-menu sm-vertical\"><li class=\"menu-item menu-item-type-post_type menu-item-object-page menu-item-105\"><a href=\"https:\/\/www.celetrix.com\/zh\/manuals-videos\/\" class=\"elementor-item\" tabindex=\"-1\">Manuals &#038; Videos<\/a><\/li>\n<li class=\"menu-item menu-item-type-post_type menu-item-object-page menu-item-107\"><a href=\"https:\/\/www.celetrix.com\/zh\/recommend-parameters\/\" class=\"elementor-item\" tabindex=\"-1\">Recommended Parameters<\/a><\/li>\n<li class=\"menu-item menu-item-type-post_type menu-item-object-page menu-item-106\"><a href=\"https:\/\/www.celetrix.com\/zh\/optimization-strategy\/\" class=\"elementor-item\" tabindex=\"-1\">Optimization Strategy<\/a><\/li>\n<li class=\"menu-item menu-item-type-post_type menu-item-object-page menu-item-104\"><a href=\"https:\/\/www.celetrix.com\/zh\/experimental-overview\/\" class=\"elementor-item\" tabindex=\"-1\">Experimental Overview<\/a><\/li>\n<li class=\"menu-item menu-item-type-post_type menu-item-object-page menu-item-108\"><a href=\"https:\/\/www.celetrix.com\/zh\/special-cells\/\" class=\"elementor-item\" tabindex=\"-1\">Special Cells<\/a><\/li>\n<\/ul>\t\t\t<\/nav>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<\/div>\n\t\t","protected":false},"excerpt":{"rendered":"<p>Electroporation by Celetrix Page Jurkat Electroporation Protocol Jurkat cells are hard to transfect with lipid reagents. With Celetrix electroporation, Jurkat cells reach &gt;90% transfection efficiency and keep growing fast afterwards. Below are our protocol and recommended parameters. Jurkat Transfection Results 18 h: GFP expression in Jurkat cells Flow cytometry: 90.27% GFP+ Jurkat cells Recommended Parameters [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"parent":51,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"elementor_header_footer","meta":{"om_disable_all_campaigns":false,"_uf_show_specific_survey":0,"_uf_disable_surveys":false,"footnotes":""},"class_list":["post-1108","page","type-page","status-publish","hentry"],"aioseo_notices":[],"_links":{"self":[{"href":"https:\/\/www.celetrix.com\/zh\/wp-json\/wp\/v2\/pages\/1108","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.celetrix.com\/zh\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/www.celetrix.com\/zh\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/www.celetrix.com\/zh\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.celetrix.com\/zh\/wp-json\/wp\/v2\/comments?post=1108"}],"version-history":[{"count":11,"href":"https:\/\/www.celetrix.com\/zh\/wp-json\/wp\/v2\/pages\/1108\/revisions"}],"predecessor-version":[{"id":2556,"href":"https:\/\/www.celetrix.com\/zh\/wp-json\/wp\/v2\/pages\/1108\/revisions\/2556"}],"up":[{"embeddable":true,"href":"https:\/\/www.celetrix.com\/zh\/wp-json\/wp\/v2\/pages\/51"}],"wp:attachment":[{"href":"https:\/\/www.celetrix.com\/zh\/wp-json\/wp\/v2\/media?parent=1108"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}